leader RNA
In Vivo SELEX of Single-Stranded Domains in the HIV-1 Leader RNA
... Experimental design. To study the role of the single-stranded regions 1, 2, and 3 in the BMH conformation of the HIV-1 5= leader RNA (Fig. 1A and B), we created full-length HIV-1 DNA genome libraries in ...
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Specific interaction between coronavirus leader RNA and nucleocapsid protein.
... To confirm the direct binding of N protein to the sequences contained within the leader RNA, we examined in vitro transcripts prepared from a pT7 plasmid containing an insert composed of[r] ...
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Nucleotide sequence and host La protein interactions of rabies virus leader RNA.
... In addition, we detected the leader RNA of rabies virus in infected cells and demonstrated an association between the leader RNA of rabies virus and the host cellular La protein.. The po[r] ...
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Vesicular stomatitis virus in Drosophila melanogaster cells: lack of leader RNA transport into the nuclei and frequent abortion of the replication step.
... 5 Vesicular Stomatitis Virus in Drosophila melanogaster Cells: Lack of Leader RNA Transport into the Nuclei and Frequent Abortion of the Replication Step SYBILLE DEZtLLtE,* DANIELLE BLON[r] ...
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Specific Interaction of Heterogeneous Nuclear Ribonucleoprotein Particle U with the Leader RNA Sequence of Vesicular Stomatitis Virus
... LS RNA bound to the 120-kDa protein from nuclear ...this RNA-protein complex immunoprecipitated the bound leader RNA when a monoclonal antibody (3G6) raised against hnRNP U protein was used ...
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Inhibition of adenovirus DNA replication by vesicular stomatitis virus leader RNA.
... Specific replication in the presence of leader RNA was restored when the concentration of adenovirus-infected or uninfected nuclear extract was increased or by the addition of purified p[r] ...
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In vitro RNA transcription by the New Jersey serotype of vesicular stomatitis virus. II. Characterization of the leader RNA.
... Previous studies using VSVInd have demonstrated that purified virions are able to synthesize a small leader RNA which is the first RNA product to be synthesized in vitro and is complemen[r] ...
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Spliced leader RNA silencing (SLS) a programmed cell death pathway in Trypanosoma brucei that is induced upon ER stress
... SL RNA transcription shut-off remains under investigation, but so far, our data suggest that the mechanism does not involve post-translational modification on ...SL RNA transcription can be ...
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Exportin 1 Mediates Nuclear Export of the Kinetoplastid Spliced Leader RNA
... Fluorescence in situ hybridization and FISH oligonucleotide synthesis. T. brucei and L. tarentolae were grown as indicated, mid-log-phase cells were pel- leted, and the supernatant was discarded. Cells were washed once ...
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Characterization of replicative intermediate RNA of mouse hepatitis virus: presence of leader RNA sequences on nascent chains.
... These data indicate that the 5' leader sequences are present at the 5' ends of the nascent mRNA strands during transcription, rather than joined to the body sequences posttranscriptional[r] ...
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Direct interactions between autoantigen La and human immunodeficiency virus leader RNA.
... * 7008 Downloaded from http://jvi.asm.org/ on November 9, 2019 by guest We have characterized the in vivo and in vitro binding of human La protein to the human immunodeficiency virus typ[r] ...
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Effects of the open reading frames in the Rous sarcoma virus leader RNA on translation.
... But not all of the mutations that altered ORFi to ORF3 and caused distinct retroviral phenotypes appreciably affected CAT and transient Pr769ag synthesis, possibly because ORFi to ORF3 a[r] ...
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Spliced Leader RNA Gene Transcription in Trypanosoma brucei Requires Transcription Factor TFIIH
... SL RNA gene promoter, ...SL RNA gene tran- scription, we first prepared transcription extract from TbX1 cells which exclusively express ...SL RNA gene tran- scription in TFIIB-depleted extract ...SL ...
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Trypanosome Spliced-Leader-Associated RNA (SLA1) Localization and Implications for Spliced-Leader RNA Biogenesis
... SL RNA digestion in permeable cells made from cells silenced for CBF5 before and after ...SL RNA as that previ- ously described by Ullu and Tschudi (36) (the oligonucleotides used are shown in ...SL ...
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Mass Spectroscopic Characterization of the Coronavirus Infectious Bronchitis Virus Nucleoprotein and Elucidation of the Role of Phosphorylation in RNA Binding by Using Surface Plasmon Resonance
... Surface plasmon resonance (Biacore) has not previously been applied to the study of the interaction of coronavirus N protein with coronavirus RNA. Therefore, we used this tech- nique to investigate the interaction ...
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jvirol00090-0376.pdf
... These data suggest that an N binding site is present within the leader RNA sequences at a site at least 65 nucleotides from the 5' end of genomic RNA and all six subgenomic mRNAs.. The l[r] ...
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Impaired RNA incorporation and dimerization in live attenuated leader variants of SIVmac239
... the leader region of HIV-1 suggesting that SL1 alone is sufficient to initiate formation of this duplex, and enhances the rate of dimerization in vitro ...on leader-RNA tertiary ...alternating ...
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Intracellular vesicular stomatitis virus leader RNAs are found in nucleocapsid structures.
... Previous studies on plusstrand leader RNA demonstrated that when cytoplasmic extracts of VSV-infected cells are centrifuged on CsCl gradients on which genome nucleocapsids band at their [r] ...
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Effects of alterations in the leader sequence of Rous sarcoma virus RNA on initiation of translation.
... We found that small perturbations of the leader length do not affect the binding and scanning of ribosomal subunits by more than about 10%, that the length and/or structure of the RSV RN[r] ...
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‘Less is more?’: a video based field study about patterned behavioral interaction and team effectiveness
... The intra-team relations and the collectivity, led by the leader, are subject to change over time. For example in response to the leader’s actions and behaviors but also in response to external factors or ...
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