Lectin pathway
Association study of mannose-binding lectin levels and genetic variants in lectin pathway proteins with susceptibility to age-related macular degeneration: a case-control study
... the lectin pathway in ...the lectin pathway was found to be crucial in triggering activation of the complement cascade after binding to natural IgM antibodies bound to neoepitopes with ...
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Potential role of the lectin pathway of complement in the pathogenesis and disease manifestations of systemic sclerosis: a case-control and cohort study
... The lectin pathway of complement is activated after binding of mannose-binding lectin (MBL) or ficolins (ficolin-1, -2, and -3) to carbohydrate patterns, acetyl groups or immunoglobulin M (IgM) bound ...
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Role of lectin pathway complement proteins and genetic variants in organ damage and disease severity of systemic sclerosis: a cross sectional study
... the lectin pathway [21, ...the lectin pathway as compared to the classical or alternative pathway has never been investigated ...
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Mutations of complement lectin pathway genes MBL2 and MASP2 associated with placental malaria
... Mannose-binding lectin (MBL, encoded by mbl2) is a serum protein involved in the initiation of innate immune responses by binding to microbial surface oligosacchar- ides, and activating the lectin ...
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Molecular defects in the mannose binding lectin pathway in dermatological disease: Case report and literature review
... MBL-MASP pathway defects in dermatological disease is sparse, and we believe our report to be the first to link MBL deficiency to recurrent infectious and inflammatory skin disease in 6 ...
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Complement in patients receiving maintenance hemodialysis: functional screening and quantitative analysis
... Recently, a commercially available kit (Wielisa®-kit, Wieslab, Lund, Sweden) was developed to measure the functional activities of the three complement pathways in parallel for screening for complement deficiencies. In ...
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Mannan binding lectin activates C3 and the alternative complement pathway without involvement of C2
... Effect of serum dilution on C3 deposition induced by CO antigen (1,000 ng/well) in C2D:21 serum (combined C2 and MBL deficiency). The serum was reconstituted with MBL (2 mg/l) and C2 (6.5 mg/l) as indicated. PNHS was ...
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Mechanisms of complement activation by dextran-coated superparamagnetic iron oxide (SPIO) nanoworms in mouse versus human serum
... classical pathway (CP), the lectin pathway (LP) or the alternative pathway ...alternative pathway (AP) via the formation of alternative C3 convertase C3bBb. Lectin pathway ...
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Novel MASP1 mutations are associated with an expanded phenotype in 3MC1 syndrome
... and lectin pathway activity of patient 3 ...alternative pathway by normal human serum (NHS) and patient 3 serum and as a negative control by NHS diluted in EDTA buffer ...
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Low ficolin-3 levels in early follow-up serum samples are associated with the severity and unfavorable outcome of acute ischemic stroke
... Additionally, a strong negative correlation was found between ficolin-3 concentration and the outcome of the disease measured with modified Rankin scale. This negative correlation indicates that low ficolin-3 levels are ...
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Original Article Prognostic value of glomerular C4d staining in patients with IgA nephritis
... mannose-binding lectin (MBL) or C4d in IgAN shows the activation of comple- ment occurring via the lec- tin pathway and negative staining shows the activation of complement occurring via the alternative ...
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Development of a glycoproteomic strategy to detect more aggressive prostate cancer using lectin-immunoassays for serum fucosylated PSA
... To determine whether serum fucosylated PSA could be used to detect AG PCa, we developed fucosylated PSA assays using lectin selection and quantitation of fuco- sylated PSA with the clinical Access Hybritech PSA ...
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Comparison of the 2D and 3D Nanostructured Lectin-Based Biosensors for In Situ Detection of Sialic Acid on Glycoproteins
... hydroxysuccinimide (NHS), N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC), poly(vinylalcohol) (PVA, Mowiol ® 4-88), sodium periodate, ethylene glycol, acetonitrile, fetuin (FET, 8.7% of sialic acid), ...
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Assessment of Altered Fucosylation of Serum Α-1-Acid Glycoprotein in Hepatocellular Carcinoma Patients by Gold-Nanoparticle Aggregation Immunoassay
... After establishing the protocol for the assay system with the commercial protein Hp, the system was modified to detect AGP glycosylation pattern in clinical samples of patients suffering from HCC and compared with the ...
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IMMUNOMODULATORY ACTIVITY OF LECTIN EXTRACTED FROM BARK OF THE BLACK MULBERRY (MORUS NIGRA)
... Cross-linked guar-gum, a galactomannan consisting of chains of (1→4) linked β-D-manose with α-D-galactose linked (1→6) as single unit side chains, has been used as an efficient, inexpensive and rapid general affinity ...
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Differentiation of Bacillus anthracis and other Bacillus species by lectins
... When cells or spores were boiled in PBS before interaction with lectin, it was found that more lectin was usually required to elicit agglutination Table 5.. These results suggest that th[r] ...
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Isolation, purification and characterization of an N acetyl D lactosamine binding mitogenic and anti proliferative lectin from tubers of a cobra lily Arisaema utile Schott
... inhibited lectin- induced ...the lectin to the recognition sites on ...assay lectin showed binding with Lac- NAc but not to T-Disaccharide and similar may be the case of asialofetuin which has both ...
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Lectin Glycan Interaction Network Based Identification of Host Receptors of Microbial Pathogenic Adhesins
... other lectin-glycan interactions, where— besides hu- man or viral interactions— other mammalian, plant, or proto- zoan glycan interactions could be explored, and also used, for example, for the discovery of new ...
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Functional Expression and Characterization of the Recombinant N-acetyl-glucosamine/N-acetyl-galactosamine-Specific Marine Algal Lectin BPL3
... In this study, the algal lectin BPL-3 ( Bryopsis plumosa lectin 3) was successfully produced 26.. using a bacterial expression system, BL21(DE3), with an artificial repeated [r] ...
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Characterization of Caenorhabditis elegans lectin-binding mutants.
... (Because surface lectin binding is restricted to small regions of the adult wild-type animal, mutants missing this lectin binding are difficult to identify in a dissecting micro- sc[r] ...
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