lentivirus vectors
Development of Multigene and Regulated Lentivirus Vectors
... for lentivirus vectors that can be regulated by cell-external ...HIV-1 vectors in primary HSFs and in human and hamster cell ...such vectors. This was observed with NL-EGFP vectors ...
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Development of Minimal Lentivirus Vectors Derived from Simian Immunodeficiency Virus (SIVmac251) and Their Use for Gene Transfer into Human Dendritic Cells
... minimal vectors SIV-RMES3 and SIV-RMES4, which combine small size and the ability to efficiently transfer a gene to cells in ...other lentivirus vectors, the minimal and efficient SIV-RMES ...
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Inhibition of Human Immunodeficiency Virus Type 1 (HIV-1) Replication by HIV-1-Based Lentivirus Vectors Expressing Transdominant Rev
... by vectors cSR9–3C and cSR7 demon- strated consistently less HIV-1 replication in cells transduced with ...similar lentivirus vectors led to very low titers (⬍10 2 CFU/ml; data not ...
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Vesicular Stomatitis Virus G-Pseudotyped Lentivirus Vectors Mediate Efficient Apical Transduction of Polarized Quiescent Primary Alveolar Epithelial Cells
... transfer vectors, while achieving a high transduction efficiency in primary cells compared to standard murine retrovirus vec- tors, has raised concerns regarding inadvertent production of replication-competent ...
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Lentivirus Vectors Pseudotyped with Filoviral Envelope Glycoproteins Transduce Airway Epithelia from the Apical Surface Independently of Folate Receptor Alpha
... Replacing the cytoplasmic tail of the MRB envelope glyco- protein with the MuLV amphotropic or FIV envelope cytoplas- mic tail. Replacing the C terminus of the MRB envelope glycoprotein with that of another glycoprotein ...
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Sequences in the Cytoplasmic Tail of the Gibbon Ape Leukemia Virus Envelope Protein That Prevent Its Incorporation into Lentivirus Vectors
... or lentivirus particles (Table ...retrovirus vectors, our analysis showed that only the nature of the T region influenced the extent of R peptide cleavage; any protein containing an MuLV T region was ...
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Pseudotyped Lentivirus Vectors Derived from Simian Immunodeficiency Virus SIVagm with Envelope Glycoproteins from Paramyxovirus
... pseudotyped lentivirus vectors because the vectors are easily concentrated by centrifugation and transduce a wide range of cell types in ...VSV-G-pseudotyped lentivirus vectors are ...
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Construction of p66Shc gene interfering lentivirus vectors and its affections on alveolar epithelial cells apoptosis induced by hyperoxia
... Results: We successfully established the p66Shc gene interfering lentivirus vectors, A549-p66ShcshRNA. The A549-p66ShcshRNA was transfected into alveolar epithelial cells, and the inhibitive effects on the ...
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A Packaging Cell Line for Lentivirus Vectors
... inducible lentivirus vector packaging cell line that was constructed in 293 cells and that constitutively expresses a tetracycline-regulated transactivator (tTA) (8, ...the lentivirus vectors with ...
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Lentivirus Vectors Using Human and Simian Immunodeficiency Virus Elements
... A lentivirus vector may prove to be a useful vehicle for delivery of a “lethal” gene (such as herpesvirus thymidine kinase) to such quiescent tumor ...for lentivirus gene ...itself. Lentivirus ...
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Oncoretrovirus and Lentivirus Vectors Pseudotyped with Lymphocytic Choriomeningitis Virus Glycoprotein: Generation, Concentration, and Broad Host Range
... Oncoretrovirus-based vectors have been the most widely used system for clinical gene therapy ...these vectors only transduce dividing cells, and within a short treatment window, most tumor cells are not ...
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Original Article β-thalassemia for translating human β-globin gene and RNA interference of α-globin gene expression by lentivirus vectors
... β-globin. Lentivirus mediated RNA interference technol- ogy make the precursor mRNA in aberrant splicing of the antisense sequence of intron 1 (such as IVS-I-110, IVS-I-6, and IVS-I-5) or intron 2 (such as ...
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Identification of a Human Immunodeficiency Virus Type 2 (HIV-2) Encapsidation Determinant and Transduction of Nondividing Human Cells by HIV-2-Based Lentivirus Vectors
... previous lentivirus vector systems have used human immunodeficiency virus type 1 (HIV-1), HIV-2 is less pathogenic in humans and is amenable to pathogenicity testing in a primate ...pression. Lentivirus ...
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Comparison of Gene Transfer Efficiencies and Gene Expression Levels Achieved with Equine Infectious Anemia Virus- and Human Immunodeficiency Virus Type 1-Derived Lentivirus Vectors
... EIAV vectors is unclear; however, it is hypothesized that gene silencing with retroviral vectors occurs through a two-step mechanism, where methylation of transcriptional elements oc- curs first, followed ...
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Study of RNA interference inhibiting rat ovarian androgen biosynthesis by depressing 17alpha-hydroxylase/17, 20-lyase activity in vivo
... According to our study, ovarian androgen biosynthesis can be partly inhibited by silencing the expression of CYP17. This may not only provide a new trend for the therapy of hyperandrogenism diseases like PCOS, but also ...
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Primate and Feline Lentivirus Vector RNA Packaging and Propagation by Heterologous Lentivirus Virions
... of lentivirus vectors for human gene therapy have been highlighted by the fact that primate lentiviruses can cross-package each other’s genomic ...HIV-1-based vectors can lead to competition for ...
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Original Article Effects of miR-144 on the biological behavior of A549 lung cancer cells
... Recent evidence has shown that miR-144 plays distinct and complex roles in different tumors [13, 14]. One study reported that miR-144 was low-expressed in colorectal cancer and elevat- ed expression of miR-144 could ...
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FetTitle: Revolution in Gene Medicine Therapy and Genome Therapy Authors and Affiliations : David Jiang 1,2 , Christine L. Xu 1,2 Stephen H. Tsang 1,2,3
... EIAV lentivirus vectors in mouse models in preclinical trials, leading to the successful transduction of genetic information in 5-20% of PR cells and reduction of lipofuscin pigment A2E accumulation [57] ...
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IL-2Rα up-regulation is mediated by latent membrane protein 1 and promotes lymphomagenesis and chemotherapy resistance in natural killer/T-cell lymphoma
... IL‑2Rα Lentivirus vectors expressing latent membrane pro- tein-1 (LMP1) were constructed as described previously ...recombinant lentivirus was collected 72 h after trans- ...
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DNA-Dependent Protein Kinase Is Not Required for Efficient Lentivirus Integration
... of lentivirus inte- ...using lentivirus vectors, although the efficiency of transduction in these cells was lower than that in established cell lines, such as 293T, HeLa, or ...attenuated ...
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