ligase chain reaction
Detection of Chlamydia trachomatis and Neisseria gonorrhoeae by ligase chain reaction based assays with clinical specimens from various sites: implications for diagnostic testing and screening
... Ligase chain reaction (LCR)-based tests for the diagnosis of Chlamydia trachomatis and Neisseria gonorrhoeae infections in men and women attending a sexually transmitted disease clinic were ...
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Urine Specimens from Pregnant and Nonpregnant Women Inhibitory to Amplification of Chlamydia trachomatis Nucleic Acid by PCR, Ligase Chain Reaction, and Transcription Mediated Amplification: Identification of Urinary Substances Associated with Inhibition and Removal of Inhibitory Activity
... PCR, ligase chain reaction (LCR), and transcription-mediated am- plification (TMA) have greatly improved our ability to diag- nose Chlamydia trachomatis infections, and in recent years, they have ...
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Duration of Persistence of Gonococcal DNA Detected by Ligase Chain Reaction in Men and Women following Recommended Therapy for Uncomplicated Gonorrhea
... Neisseria gonorrhoeae infection remains relatively common in the United States, representing a public health challenge. Ligase chain reaction (LCR) is both highly sensitive and specific for the ...
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Pooling Urine Samples for Ligase Chain Reaction Screening for Genital Chlamydia trachomatis Infection in Asymptomatic Women
... The accuracy of pooling urine samples for the detection of genital Chlamydia trachomatis infection by ligase chain reaction (LCR) was examined. A model was also developed to determine the number of ...
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Direct detection of Mycobacterium tuberculosis complex in clinical samples from patients in Norway by ligase chain reaction
... the ligase chain reaction (LCR) (3, 4, ...a reaction mixture consisting of four oligonucleotide probes, thermo- stable ligase and polymerase, and individual nucleotides in ...filled, ...
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Comparison of Performances of Two Commercially Available Tests, a PCR Assay and a Ligase Chain Reaction Test, in Detection of Urogenital Chlamydia trachomatis Infection
... or ligase chain reaction (LCR) technology is demanding with respect to laboratory facilities and equipment, but stan- dardized easy-to-use kits are ...
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Detection of Chlamydia trachomatis infection in urine samples from men and women by ligase chain reaction
... a ligase chain reaction (LCR)-based assay with plasmid primers was examined with a group of patients attending a sexually transmitted disease clinic in Amsterdam, The ...
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Pooling of Urine Samples for Screening for Neisseria gonorrhoeae by Ligase Chain Reaction: Accuracy and Application
... The accuracy of detection of genital Neisseria gonorrhoeae infection in pooled urine samples by ligase chain reaction (LCR) was examined in three populations. Firstly, urine specimens from 300 female ...
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Use of Ligase Chain Reaction with Urine versus Cervical Culture for Detection of Chlamydia trachomatis in an Asymptomatic Military Population of Pregnant and Nonpregnant Females Attending Papanicolaou Smear Clinics
... by ligase chain reaction (LCR) (2, 8, 14, 20, 31, 34), which with its easily obtained specimen is a cost-effective method for screening programs for asymptomatic women ...
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Rapid Diagnosis of Extrapulmonary Tuberculosis by Ligase Chain Reaction Amplification
... scription-mediated amplification (2, 18, 19, 42). These tech- niques supply all of the necessary reagents for sample prepa- ration and for amplification and detection. Recently, ligase chain reaction ...
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Sensitive Detection of HBsAg Mutants by a Gap Ligase Chain Reaction Assay
... Hepatitis B virus (HBV) surface gene variants have been associated with diagnostic escape and immune escape following vaccination. The most common mutation observed in these variants is a glycine-to-arginine substitution ...
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Performance of Transcription Mediated Amplification and Ligase Chain Reaction Assays for Detection of Chlamydial Infection in Urogenital Samples Obtained by Invasive and Noninvasive Methods
... the ligase chain reaction (LCR), the performance characteristics of the Gen-Probe Chlamydia trachomatis transcription-mediated amplification (TMA) assay were evaluated with endocervical, urine, and ...
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Abstract
... The essential material for Chlamydia tracho− matis detection is cervical smears in women and urethral smears in both men and women [1, 3, 5, 6]. Of the various methods of detecting Chlamydia trachomatis presence, two ...
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Detection of Chlamydia trachomatis andNeisseria gonorrhoeae by Enzyme Immunoassay, Culture, and Three Nucleic Acid Amplification Tests
... The purpose of this study was to evaluate and compare three commercially available nucleic acid amplifi- cation tests (NAATs) for the detection of Neisseria gonorrhoeae and Chlamydia trachomatis. Roche PCR and Becton ...
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Comparing First Void Urine Specimens, Self Collected Vaginal Swabs, and Endocervical Specimens To Detect Chlamydia trachomatis and Neisseria gonorrhoeae by a Nucleic Acid Amplification Test
... by ligase chain reaction as well as to determine the prevalence of Trichomonas vaginalis by culture in a large and diverse national sample of non-health-care-seeking young women entering the ...
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Chain reaction final report
... the Chain Reaction project was to inform the local and international education community about the IBSE learning approach supported by the project, as well as the impact of the implementation of the project ...
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Universal TA Cloning
... of ligase-dependent methods employed for the covalent joining of a DNA insert to a plasmid ...the ligase-dependent methods require multiple purification and/or enzymatic modification ...
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Sulfur mustard induces expression of metallothionein-1A in human airway epithelial cells
... Next, we examined the expression of MT-1A in the sulfur mustard-injured patients. Because the controls had expressed identical levels of the gene, all of them were used for com- parison with the results in the sulfur ...
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Nested polymerase chain reaction technique for the detection of Gpc3 and Afp mRNA in liver cancer micrometastases.
... first chain took place using a reaction mixture comprising: 1 µL dithiothreitol, 4 µL 5X buffer, 200 U (1 µL) Moloney murine leukemia virus reverse transcriptase (M-MLV RT), 18-20 ...
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Rapid screening for Chlamydia trachomatis infection by detecting α mannosidase activity in urogenital tract specimens
... [32], α-D-mannosidase activity was invisible by naked eyes with 6-chloro-3-indolyl-α-D-mannoside as sub- strate. This may be because the extracellular α-D-mannosi- dase from these organisms is much less or the enzyme ...
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