live imaging
Clarification of mural cell coverage of vascular endothelial cells by live imaging of zebrafish
... Fig. 1. Generation of Tg zebrafish lines for live imaging of MCs. (A) A schematic structure of the BAC clone (CH1073-606I16) used to generate Tg zebrafish lines for live imaging of MCs. cDNA ...
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Live imaging reveals a biphasic mode of dissemination of Borrelia burgdorferi within ticks
... It is a central tenet of Lyme disease pathogenesis that Borrelia transition between 2 dramatically different transcriptional pro- grams with the onset of tick feeding, with motility being a salient, distinguishing ...
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Live imaging of multicolor labeled cells in Drosophila
... with live imaging. An alternative, live imaging- compatible method would be to create, within each cell, a concentration gradient of fluorescence towards the plasma ...to imaging, data ...
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In toto live imaging of mouse morphogenesis and new insights into neural tube closure
... and live imaging of other vertebrate embryos, such as chick and Xenopus, have highlighted two major morphological processes: (1) bending or inflection of the neural folds at the medial hinge point and at ...
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Non invasive long term fluorescence live imaging of Tribolium castaneum embryos
... previous live imaging attempts have addressed only specific questions and no long-term live imaging data of Tribolium embryogenesis have been ...fluorescence live imaging of ...
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Live imaging of developing mouse retinal slices
... of live imaging, the NBL of retinal slice cultures does not suffer extensive cell death that would hinder interpretation of the INM time lapse ...Subsequent live imaging of INM, utilizing the ...
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Live Imaging of Host Parasite Interactions in a Zebrafish Infection Model Reveals Cryptococcal Determinants of Virulence and Central Nervous System Invasion
... Through live-imaging observations and genetic knockdown, we establish that macrophages are the primary immune cells responsible for responding to and containing acute cryptococcal infec- ...vae. Live ...
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Live imaging and tracking of genome regions in CRISPR/dCas9 knock in mice
... to live animals, we generated mouse strain with dCas9-EGFP ubiquitously expressed in multiple tissues, which bypasses the delivery issues with large dCas9 protein into ...in live animals is significantly ...
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Live imaging of ERK signalling dynamics in differentiating mouse embryonic stem cells
... Fig. 1. Monitoring ERK activity dynamics in single live ESCs. (A) Immunofluorescence of Rex1-GFP reporter ESCs immunostained for pERK (red) and DAPI (blue), cultured in serum+LIF alone or additionally treated with ...
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Cationic Amphipathic Antimicrobial Peptides Perturb the Inner Membrane of Germinated Spores Thus Inhibiting Their Outgrowth
... Spore-forming bacteria of the Bacillales and Clostridiales order produce resilient dormant spores in response to stressful environmental conditions, such as nutrient deprivation, that can withstand numerous stress ...
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Membrane Active Peptides and Their Biophysical Characterization Fatma Gizem Avci 1, Berna Sariyar Akbulut1 , Elif Ozkirimli 2
... In the last 20 years, an increasing number of studies have been reported on membrane active peptides, which exert their biological activity by interacting with the cell membrane either to disrupt it and lead to cell ...
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Investigating the Role of F-Actin in Human Immunodeficiency Virus Assembly by Live-Cell Microscopy
... Figure 3A and B and Movie S4 in the supplemental material summarize the results obtained for LAT-B. Typically, within 100 frames (200 s) after drug addition, filamentous actin structures were disrupted and converted into ...
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Brain on the stage - Spotlight on nervous system development in zebrafish: EMBO practical course, KIT, Sept. 2013
... lectures, live imaging in the zebrafish retina was shown as an approach to investigate individual neural stem cell (NSC) behaviour in the developing and mature vertebrate ...simultaneous imaging of ...
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Replication-Competent Influenza A Virus That Encodes a Split-Green Fluorescent Protein-Tagged PB2 Polymerase Subunit Allows Live-Cell Imaging of the Virus Life Cycle
... proteins. Live imaging of cells infected with a split-GFP-based virus demonstrated, at 3 to 7 h postinfection, a pericentriolar ac- cumulation of viral RNPs followed by a wide distribution throughout the ...
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A Portable Live Cell Culture and Imaging System with Optional Umbilical Bioreactor Using a Modified Infant Incubator
... Images obtained from this system are dependent both on the quality of the microscope and objectives, as well as on the imaging method. The Olympus IMT2 microscope used for our experiments was ideally suited for ...
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Pharmacokinetics on a microscale: visualizing Cy5-labeled oligonucleotide release from poly(<em>n</em>-butylcyanoacrylate) nanocapsules in cells
... lines. Live cell imaging with CLSM for colocalization studies with the three different cell lines clearly demonstrated an increase in oligonucleotides released from the nanocapsules, and accu- mulation at ...
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Live Cell Imaging of Phosphoinositide Dynamics and Membrane Architecture during Legionella Infection
... Live Cell Imaging of Phosphoinositide Dynamics and Membrane Architecture during Legionella Infection Live Cell Imaging of Phosphoinositide Dynamics and Membrane Architecture during Legionella Infectio[.] ...
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Fluorescence lifetime imaging of E combretastatin uptake and distribution in live mammalian cells
... Overall these results show that the E-combretastatins are rapidly (within minutes) accumulated by live mammalian cells and reach intracellular concentrations many times those in the medium. This is likely to be a ...
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Illuminating the Sites of Enterovirus Replication in Living Cells by Using a Split-GFP-Tagged Viral Protein
... for live-cell imaging by monitoring the development of ROs and the disintegration of the Golgi apparatus, as visualized using GM130- mCherry, in infected cells in real ...
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Hermann, Stefanie Monika (2018): Functional and biochemical characterization of the epigenetic inheritance of an artificial centromere. Dissertation, LMU München: Medizinische Fakultät
... a live cell imaging approach, the staining of EBNA1 is not ...In live cell imaging, EBNA1 signals are not amplified by sequential antibody ...in live cell ...
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