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living cells

APOBEC3G Impairs the Multimerization of the HIV-1 Vif Protein in Living Cells

APOBEC3G Impairs the Multimerization of the HIV-1 Vif Protein in Living Cells

... in living cells through its PPLP ...transfected cells (80 to 90%) but were also present in the nucleus (10 to 20%), in agreement with previous observations ...

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Model based design of RNA hybridization networks implemented in living cells

Model based design of RNA hybridization networks implemented in living cells

... Finally, RNA hybridization networks might be very use- ful to perform bio-logical computations in living cells, due to their ability for storing and retrieving information (e.g. Supplementary Figure S15 ...

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Recent development of fluorogenic probes for carbon  monoxide imaging in living cells

Recent development of fluorogenic probes for carbon monoxide imaging in living cells

... in living systems has discovered that these gases perform essential biological functions and are associated with various diseases in ...in living systems in a non-invasive ...

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Versatility of RNA-Binding Proteins in Living Cells

Versatility of RNA-Binding Proteins in Living Cells

... Riki Kurokawa have established a novel assay to detect interaction of RBPs with RNA, using biotinylated RNA oligos to capture RBPs with Western blot of specific antibodies against RBPs. The assay detects RNA binding more ...

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Multiplicity in Long Noncoding RNA in Living Cells

Multiplicity in Long Noncoding RNA in Living Cells

... the living human ...the living cells are different from those in in vitro ...the living human ...HeLa cells by a method utilizing toxic protein, streptolysin O (SLO), which enables to ...

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A Fluorescence Ratiometric Probe for Detection of  Cyanide in Water Sample and Living Cells

A Fluorescence Ratiometric Probe for Detection of Cyanide in Water Sample and Living Cells

... the cells exhibited strong fluorescence in the green channel (Figure 10 a)), and nearly no fluorescence in the red channel (Figure 10 ...the cells exhibited strong fluorescence in the red channel (Figure 10 ...

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Novel Quantitative Approach for Predicting mRNA/Protein Counts in Living Cells

Novel Quantitative Approach for Predicting mRNA/Protein Counts in Living Cells

... DOI: 10.4236/am.2017.88085 1129 Applied Mathematics neity [7]. But the confirmation came later with [9], [10] and [11] who showed that mRNA and protein variability may lead to important a source of noise in biology. ...

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Subcellular Localization of Thiol Capped CdTe Quantum Dots in Living Cells

Subcellular Localization of Thiol Capped CdTe Quantum Dots in Living Cells

... Abstract Internalization and dynamic subcellular distri- bution of thiol-capped CdTe quantum dots (QDs) in living cells were studied by means of laser scanning confocal microscopy. These unfunctionalized ...

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Compartmental Genomics in Living Cells Revealed by Single-Cell Nanobiopsy

Compartmental Genomics in Living Cells Revealed by Single-Cell Nanobiopsy

... of living single cells in heterogeneous cell populations is essential for next generation analysis of cellular circuitry and ...from living cells with minimal disruption of the cellular ...

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Mapping Interactions between mRNA Export Factors in Living Cells

Mapping Interactions between mRNA Export Factors in Living Cells

... To establish whether the pattern of interaction observed between Nxf1 and Chtop was a more general property of other mRNA export factors which interact with Nxf1, we also monitored the Alyref-ECFP:Nxf1-EYFP interaction ...

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Spatial and structural metrics for living cells inspired by statistical mechanics

Spatial and structural metrics for living cells inspired by statistical mechanics

... single living cells, and other organelles inside the cell can be treated ...single cells in geometric ...single living cells in terms of pair correlation func- tions for lysosomes, ...

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Thermodynamic Jump from Prebiotic Microsystems to Primary Living Cells

Thermodynamic Jump from Prebiotic Microsystems to Primary Living Cells

... Abstract: It is proposed that the primary living cells (“probionts”) cannot emerge of organic substance simply by continuous chemical complication of prebiotic macromolecules and microsystems. The ...

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Using microscopy to manipulate and visualize signal transduction in living cells

Using microscopy to manipulate and visualize signal transduction in living cells

... Elucidation of complex signaling pathways requires precise spatio-temporal information on the activation of key signaling components in live cells and tissues. While most current methods for studying protein ...

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Dynamic properties of independent chromatin domains measured by correlation spectroscopy in living cells

Dynamic properties of independent chromatin domains measured by correlation spectroscopy in living cells

... Despite the impressive advancements in the field, details on the organization and dynamic properties of chromatin in single living cells are elusive. However, the plasticity of chromatin organization is a ...

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mRNA analysis of single living cells

mRNA analysis of single living cells

... single living cells and the results of five single cells are shown in ...single living cells, the response of rat VNOF90 cells to se- rum has been used as a model for studying ...

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Localization of CD4 and CCR5 in Living Cells

Localization of CD4 and CCR5 in Living Cells

... target cells as observed by deconvolution ...HeLa cells with a native CD4 construct, cells were fixed and stained with an anti-CD4 anti- body (Sigma, ...live cells expressing the CD4-YFP ...in ...

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Visualizing Herpesvirus Procapsids in Living Cells

Visualizing Herpesvirus Procapsids in Living Cells

... Fusing GFP to VP24 achieves two useful advances. First, unlike fusions to VP26 and pUL25, which decorate the capsid surface with GFP, the fluorescent protein is contained within the capsid shell. By leaving the outer ...

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Communication mechanism between living cells

Communication mechanism between living cells

... siderably in shape, size, chemical composition, and function. Despite these differences, neurons consist of the three main parts viz. a) soma or cell body, b) an axon, and c) dendrites. Figure 1 shows the structure of a ...

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Real-time visualization of heterotrimeric G protein Gq activation in living cells

Real-time visualization of heterotrimeric G protein Gq activation in living cells

... The spatiotemporal characteristics of heterotrimeric G protein activation are difficult to monitor, because direct measurement of GTP binding and hydrolysis is not feasi- ble in living cells [48]. Instead, ...

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Engineering of RNA sensors and actuators in living cells

Engineering of RNA sensors and actuators in living cells

... The incorporation of riboregulators within PIE group I ribozymes enabled the genera- tion of circular sRNA regulators. The ribozymes splice correctly despite the addition of a sRNA cargo between the exon sequences, and ...

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