LLC-PK1 cells
All trans retinoic acid stimulates synthesis of cyclic ADP ribose in renal LLC PK1 cells
... Cyclic adenosine diphospho-ribose (cADPR) triggers Ca2+ release from intracellular stores and is therefore proposed to function as a second messenger in cellular signaling; however, an extracellular stimulus, i.e., first ...
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Studies of renal injury II Activation of the glucose transporter 1 (GLUT1) gene and glycolysis in LLC PK1 cells under Ca2+ stress
... Injury to the renal proximal tubule is common and may be followed by either recovery or cell death. The survival of injured cells is supported by a transient change in cellular metabolism that maintains life even ...
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ATP receptor regulation of adenylate cyclase and protein kinase C activity in cultured renal LLC PK1 cells
... intact LLC-PK1 renal epithelial cells, a nonhydrolyzable ATP analogue, ATP gamma S, inhibits AVP-stimulated cAMP ...In LLC-PK1 membranes, several ATP analogues inhibit basal, GTP-, ...
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The effect of acyclovir on the tubular secretion of creatinine in vitro
... Figure 5 The effect of acyclovir on creatinine transport across porcine renal proximal tubular cell (LLC-PK1) monolayers. The transport (basolateral-to-apical direction) of creatinine was assessed in ...
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Differential profile of p38 mapk pathway in changeoxidative stress, nitric oxide production and celldeath in llc pk1 and mdck cells: case amphotericin b and cyclosporine
... when LLC-PK1 and MDCK were preincubated which PD169316 (Tables 1 and ...in LLC-PK1 cells incubated with cyclosporine and MDCK cellswere more sensitive to amphotericin (tables 1 and ...
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Clusterin promotes the aggregation and adhesion of renal porcine epithelial cells
... In this study, a single cell suspension of renal epithelial LLC-PK1 cells was treated with purified human clusterin, resulting in time- and dose-dependent cell aggregation.. Electron mic[r] ...
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Entry and release of transmissible gastroenteritis coronavirus are restricted to apical surfaces of polarized epithelial cells.
... To determine if the entry of TGEV in LLC-PK1 cells is restricted to a specific membrane domain, we analyzed virus-specific protein synthesis in filter-grown cells infected from the apica[r] ...
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Activation of Caspases in Pig Kidney Cells Infected with Wild-Type and CrmA/SPI-2 Mutants of Cowpox and Rabbitpox Viruses
... infected LLC- PK1 ...that LLC-PK1 cells infected with wt RPV contain apoptotic proteolytic activity which does not become activated in the context of CPV infection in the presence of ...
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Standardization of in vitro cell-based model for renal ischemia and reperfusion injury
... In order to create oxygen deficiency that leads to impaired oxidative phosphorylation, ultimately resulted in ATP depletion was created in LLC PK1 cells by using two enzymes system consisting of ...
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Comparison of Two Renal Cell Lines (NRK 52E and LLC PK1) as Late Stage Apoptosis Models
... NRK-52E cells were investigated separately using the DNA laddering assay, apoptosis could be detected with TBT-O and TBT-Cl in detached cells ...for LLC- PK1 cells, the degree and ...
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Energy thresholds that determine membrane integrity and injury in a renal epithelial cell line (LLC PK1) Relationships to phospholipid degradation and unesterified fatty acid accumulation
... This study related ATP levels with membrane damage, lipid abnormalities, and cell death in energy-depleted LLC-PK1 cells. Oxidative phosphorylation was inhibited by antimycin A, and glycolysis was ...
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Merlin Phosphorylation by p21-activated Kinase 2 and Effects of Phosphorylation on Merlin Localization
... from LLC-PK1 cells ectopically expressing wild-type merlin were used in Western blot analysis with either SC331 or HM2175 ...NIH3T3 cells, immunoprecipitated, and analyzed by Western blot, ...
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Basolateral localization and export activity of the human multidrug resistance associated protein in polarized pig kidney cells
... It is tempting to speculate about a possible relationship be- tween the probenecid- and sulfinpyrazone-inhibitable excre- tion of organic anions in the kidney and MRP. Based on the presence of several markers, ...
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Different mechanisms of nephrotoxicity caused by amphotericin b and cyclosporin an involving pka and p38 mapk
... MDCK cells, the percentage of DNA fragmentation decreased, showing that when MDCK cells were treated with AmB or CsA, probably p38 MAPK and PKA were involved in cell death caused by these compounds (Figures ...
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Porcine Deltacoronavirus Engages the Transmissible Gastroenteritis Virus Functional Receptor Porcine Aminopeptidase N for Infectious Cellular Entry
... BHK-pAPN cells by 36 h postinoculation (hpi), but no viral antigens were found in challenged Vero or BHK-21 cells ...Vero-pAPN cells with an efficiency of 25 to 30% and BHK-pAPN cells with an ...
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Differential regulation of doxorubicin-induced mitochondrial dysfunction and apoptosis by Bcl-2 in mammary (MTLn3) cells
... Neo cells indi- cated that doxorubicin induced a dissipation of ⌬ of ⬃ 60% in AV ⫺ /PI ⫺ ...Bcl-2 cells (Fig. 8A). The ⌬ of apoptotic AV ⫹ /PI ⫺ cells remained unchanged under all condi- tions in ...
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<p>In vitro Transport Ability of <em>ABCC2</em> (G1249A) Polymorphic Variant Towards Anticancer Drugs</p>
... cancer cells is involved in the overexpression of MRP2 ...research, LLC-PK1 derived fro, a pig kidney, has been shown to be useful to observe transport proteins ...selected LLC-PK1 cell ...
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Guanosine supplementation reduces apoptosis and protects renal function in the setting of ischemic injury
... into cells and then proceeds via the salvage pathway to generate guanine nucleotides ...in LLC-PK1 cells argue against significant beneficial hemodynamic or anti-inflammatory properties of ...
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Treatment of Ischemia Reperfusion Injury with RNA Interference
... of cells remained ...The PK1 cells were found to be quite susceptible to the antimycin A and the cell injury appeared to worsen with the recovery phase in normal, glucose containing, ...the ...
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Claudin 16 and claudin 19 interact and form a cation selective tight junction complex
... inherited disorder caused by mutations in the genes encoding the TJ proteins claudin-16 (CLDN16) and CLDN19; however, the mechanisms underlying the roles of these claudins in mediating paracellular ion reabsorption in ...
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