Long-term bone marrow culture
Hematopoietic microenvironment Origin, lineage, and transplantability of the stromal cells in long term bone marrow cultures from chimeric mice
... of bone marrow into genetically anemic Wx/Wv fetuses, a technique that essentially precludes engraftment of nonhematopoietic ...in long-term bone marrow culture derived ...
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A simple and reliable protocol for long-term culture of murine bone marrow stromal (mesenchymal) stem cells that retained their in vitro and in vivo stemness in long-term culture
... We culture the primary mBMSCs using the traditional method of plastic adherence after avoiding the fast-adhered HPCs ...for long term (Fig. 1a). We have applied this protocol to culture and ...
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Cytokine dependent long term culture of highly enriched precursors of hematopoietic progenitor cells from human bone marrow
... Human marrow cells positive for the CD34 antigen but not expressing HLA-DR, CD15, or CD71 antigens were ...liquid culture system supplemented with 48-hourly additions of recombinant interleukins IL-1 alpha, ...
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Osteoclast like cells form in long term human bone marrow but not in peripheral blood cultures
... the bone marrow cultures multinucleated ...in long-term human bone marrow ...the culture conditions used in these ...
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Molecular and cellular characteristics of human and non-human primate multipotent stromal cells from the amnion and bone marrow during long term culture
... the bone marrow [9], whereas other groups showed decreased expression in middle and later passages of chorion MSCs ...human bone marrow samples owing to the mentioned ...
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A preclinical study—systemic evaluation of safety on mesenchymal stem cells derived from human gingiva tissue
... in long-term culture but also benefited from additional advantages in addressing ethical concerns of access compared with MSCs isolated from other ...to bone marrow mesenchymal stem ...
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Paracrine regulation of fat cell formation in bone marrow cultures via adiponectin and prostaglandins
... normal bone marrow and have long been suspected to have an influence on hematopoiesis ...in bone mar- row, affecting hematopoiesis both directly and indi- ...in culture, and fully ...
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Hepatocyte growth factor (HGF) and stem cell factor (SCF) maintained the stemness of human bone marrow mesenchymal stem cells (hBMSCs) during long-term expansion by preserving mitochondrial function via the PI3K/AKT, ERK1/2, and STAT3 signaling pathways
... vitro culture reduce their effectiveness and create unknown risks [9, ...human bone marrow mes- enchymal stem cells (hBMSCs) are difficult to expand in vitro and prone to cell aging, possibly due to ...
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CGH and SNP array using DNA extracted from fixed cytogenetic preparations and long-term refrigerated bone marrow specimens
... Some copy number aberrations could only be identi- fied by visual examination of the B allele frequencies (an example shown in Figure 5). Mosaicism for a 7q dele- tion in the U937 specimen allowed us to assess the level ...
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Expression of human bone related proteins in the hematopoietic microenvironment
... between bone and bone marrow, we hypothesized that the human bone marrow may function as a source (or reservoir) of bone-forming progenitor ...the bone marrow which ...
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A clinical observation of Chinese chronic myelogenous leukemia patients after discontinuation of tyrosine kinase inhibitors
... performed long-term culture-initiating cell assays with CD34+ cells obtained from the bone marrow of patients with sustained undetectable molecular residual disease for 3 years or more ...
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Differentiation of bone marrow mesenchymal stem cells into chondrocytes after short term culture in alkaline medium.
... one-week culture in alkaline media. By continuing the culture of P1 MSCs in pH=8, the expression of type II collagen decreased, while expression of osteopontin remained partially ...in culture dish ...
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Intra articular injection of two different doses of autologous bone marrow mesenchymal stem cells versus hyaluronic acid in the treatment of knee osteoarthritis: long term follow up of a multicenter randomized controlled clinical trial (phase I/II)
... gous bone marrow mesenchymal stem cells versus hyaluronic acid in the treatment of knee osteoarthritis: multicenter randomized controlled clinical trial (phase ...
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Responses of mouse femoral bone marrow granulocyte macrophage colony forming cells (CM CFC) to X rays and restriction endonucleases
... The marrow cells were then renoved by inserting a 23G x inch needle into the inter condylar notch and flushed with 1 ml of either partially or fully supplemented medium through the bone cavity by using a 1 ...
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Bone Marrow Transplant Long Term Survivors’ Satisfaction with Quality of Life: Comparison with a Control Group
... A cross-sectional study was conducted with 478 participants, of which 214 belonged to the group of transplant survivors and 264 healthy individuals that were selected among the general population and constituted the con- ...
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Long-term effects of ruxolitinib versus best available therapy on bone marrow fibrosis in patients with myelofibrosis
... The highly selective oral Janus kinase (JAK) 1/JAK2 inhibitor ruxolitinib has been shown in two phase 3 studies to reduce splenomegaly and symptom burden in patients with intermediate-2 or high-risk disease compared with ...
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Long term persistence of donor nuclei in a Duchenne muscular dystrophy patient receiving bone marrow transplantation
... that bone marrow cells transplanted into lethally irra- diated mdx mice, the mouse model of DMD, can become part of skeletal muscle ...human marrow cells also have this ability is ...received ...
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Treatment of Lower Back Pain with Bone Marrow Concentrate
... most prominent when sitting or lying on his stomach. MRI of his lumbar spine demonstrated moderate L3-L4 central stenosis due to a broad based disc bulge and facet degenerative change with hypertrophy. He underwent an ...
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Inhibition of osteoclast like cell formation by bisphosphonates in long term cultures of human bone marrow
... responsiveness, and reactivity with specific MAbs). The five bisphosphonates that were tested strongly inhibited 1,25-dihydroxyvitamin D3-stimulated formation of these cells with the same relative potencies as they ...
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Bone marrow is a major site of long-term antibody production after acute viral infection.
... In this study, we examined the anatomical site of long-term antibody production by using the lymphocytic choriomeningitis virus (LCMV) model system. LCMV infection of mice pro- vides a useful model to study ...
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