Long term culture
Long term culture of pluripotent stem cell derived human neurons on diamonds" A substrate for neurodegeneration research and therapy
... Comparing the size and number of neurons grown on undoped and boron-doped diamond we found that boron-doped diamond has no adverse in fl uence on cell survival and neurite formation. However, this does not preclude the ...
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A simple and reliable protocol for long-term culture of murine bone marrow stromal (mesenchymal) stem cells that retained their in vitro and in vivo stemness in long-term culture
... We culture the primary mBMSCs using the traditional method of plastic adherence after avoiding the fast-adhered HPCs ...for long term (Fig. 1a). We have applied this protocol to culture and ...
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Evaluation of a multiwell perfused bioreactor system for the long-term culture of mouse hepatocytes
... hepatocyte culture is one of our ultimate goals, the system has limitations that need to be addressed if the system is to replace traditional two-dimensional ...independent culture system with its own flow ...
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Long-term culture of human liver tissue with advanced hepatic functions
... 3D culture systems have shown some progress in extending the functions of pri- mary hepatocytes, the functional lifetime of current systems is still limited for critical applications ...(e.g., ...
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Original Article Production of proinflammatory mediators by human neutrophils during long-term culture
... during long-term culture in neutrophil functions and ...of culture and number of cells in early and late apoptosis were ...in long-term colture, although time- length of the ...
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Long-term culture of cholangiocytes from liver fibro-granulomatous lesions
... of culture and stopped at the end of the first ...of culture produced predomi- nantly macrophages with progressive proliferation of connective tissue ...
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Propagation of human germ stem cells in long-term culture
... In this study, we applied specificity of PLZF for human germ stem cells to identify these cells. Presence of SSCs clusters during the entire culture was proved by studding the expression of spermatogonial genes ...
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Long term culture of MDCK strains alters chromosome content
... The data presented here illustrate the fact that cell populations can considerably drift. The main change observed for MDCK I and MDCK II cells maintained for a long time in culture, was the emergence of an ...
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Regeneration of TiO2 Nanotube Arrays after Long-Term Cell and Tissue Culture for Multiple Use – an Environmental Scanning Electron Microscopy (ESEM) Survey of Adult Pig Retina and beyond
... Successful long-term culture of guinea pig adult retinal tissue is one of the key achievements that became feas- ible by introduction of nanotube substrates as reported by us previously ...
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Long Term In Vitro Culture of the Syphilis Spirochete Treponema pallidum subsp pallidum
... achieving long-term culture of ...for culture of Borrelia (relapsing fever) and Borreliella (Lyme disease) isolates (39, ...for long-term T. pallidum culture and, if so, ...
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Long-term Cultured Human Neural Stem Cells Undergo Spontaneous Transformation to Tumor-Initiating Cells
... [5] and Florian [6]. NSCs have been considered a promising tool for cell transplantation therapy and tissue engineering [7]. In order to obtain a sufficient amount of NSCs for clinical use, extensive in vitro expansion ...
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spheroids using agas permeable plate and embedded
... Ishikawa cell line during long-term culture: Proposal for an in vitro clonal evolution model of tumor cells.. Organoids in cancer research.[r] ...
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Unregulated proliferation of primitive neoplastic progenitor cells in long term polycythemia vera marrow cultures
... inoculum long-term marrow ...the long-term marrow culture system supports the continuous proliferation of primitive neoplastic progenitor cells from PV patients for many ...normal ...
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Clinical Nurse Leader as Outcomes Manager in the Emergency Department: Redesigning the Triage Process to Reduce the Left Without Being Seen (LWBS) Rate
... English originated from the Indo-European language family, while Chinese emerged from the Sino-Tibetan language family. Their lexical, syntactic, contextual, and grammatical dissimilarities are rooted in the distinctions ...
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Realtionships between Fiancial and Learning and Growth Perspectives in BSC
... According to researches, satisfaction of employees is connected with various factors (for example suitable surrounding in workplace, creating good atmosphere and climate in workplace, communication between subordinates ...
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Sociology Slide 1.pptx
... long-term social processes of organizations, institutions, and broad social patterns,.. including the state, social class, the family, the economy, culture and society..[r] ...
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Culture and corporate governance, a theoretical perspective
... There is a lot of logic in Sweeney's interpretations of the work of Chan and Cheung (2008) as such logic has been confirmed by other empirical studies. Hence, the adaptation of Sweeney's definition of corporate ...
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Long term in vitro 3D hydrogel co culture model of inflammatory bowel disease
... To determine potential mucin production, cell cultures following 3, 4, and 7 weeks in culture were stained using Alcian blue and periodic acid Schiffs (AB/PAS). Alcian blue positively stains the hydrogel, due to ...
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phlp_entire.doc
... guideline' OR 'prescription' AND ('long-term care' OR 'homes for the aged' OR 'nursing home' OR 'nursing homes' OR 'long term care home' OR 'long term care' OR 'longterm care' OR 'elde[r] ...
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TOWARDS SUCCESSFUL ADAPTATION OF PLASMODIUM KNOWLESI TO LONG TERM IN VITRO CULTURE IN HUMAN ERYTHROCYTES
... direct culture in 100% human RBCs resulted in very low growth rates and a complete loss of parasites within a week 25 ...in-vitro culture for ...continuous culture and obtain an efficient parasite ...
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