Loop-mediated isothermal amplification assay (LAMP)
Visual detection of H3 subtype avian influenza viruses by reverse transcription loop mediated isothermal amplification assay
... The viral DNA/RNA extracted from the different sub- type AIVs and NDV, IBV, ILTV, MG were detected using the RT-LAMP assay to evaluate the specificity. The results of the negative sample showed no typical ladder ...
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Rapid identification of Aconitum plants based on loop mediated isothermal amplification assay
... LAMP assay included an initiation reaction of 10 min followed by amplification for 20 min at the isothermal reaction temperature of 65 ...the assay can be used for 1 pg of purified ...
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Detection of helminths by loop-mediated isothermal amplification assay: a review of updated technology and future outlook
... LAMP assay gave 100% concordance with the results obtained by nested RT-PCR when testing parasite DNA extracted from hydatid cysts from domestic ani- mals and humans, which highlights a brilliant future in ...
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Comparison of loop mediated isothermal amplification assay and smear microscopy with culture for the diagnostic accuracy of tuberculosis
... TB-LAMP appears to be suitable and good candidate for use in developing countries for its sensitivity and rapidity [7]. Several LAMP assays targeting different M.tuberclosis genes have been evaluated for the diagno- sis ...
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Development of a Loop Mediated Isothermal Amplification Assay for Rapid Detection of BK Virus
... LAMP and PCR assays. PCRs were performed using the Takara PCR protocol (TakaraMirus Bio Inc., WI). The reaction mixtures (20 l) contained 10 l of Takara master mix, 0.67 l each of 10 mM forward and reverse primers, ...
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Development and Evaluation of Reverse Transcription Loop Mediated Isothermal Amplification Assay for Rapid and Real Time Detection of Japanese Encephalitis Virus
... RT-LAMP assay has dem- onstrated a higher sensitivity by correctly detecting nine addi- tional positive samples with low levels of virus that were missed by ...
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Original Article Development and validation of a rapid and simple fluorescence real-time loop-mediated isothermal amplification assay for detection of Burkholderia pseudomallei
... The LAMP method requires much less time than traditional culture and is more cost-effec- tive [29, 30]. In this study, the LAMP assay was shown to be completed in a short time and that the reaction products could ...
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Loop mediated isothermal amplification assay for rapid detection of staphylococcus aureus
... LAMP assay was that it only requires a heating block, which is easily available in most ...LAMP assay could be determined by unaided eye without requiring special ...
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Application of Real Time Loop Mediated Isothermal Amplification Assay on Dried Blood Spots in the Detection of HCV RNA among High Risk Patients
... HCV specific primers were highly specific for the detection of HCV RNA with no cross reaction. HCV RT-LAMP primers did not amplify or cross reacted with any of the Hepatitis B (DNA), Hepatitis A and Chikungunya viral RNA ...
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Rapid detection of newly isolated Tembusu related Flavivirus by reverse transcription loop mediated isothermal amplification assay
... The RT-LAMP reaction was done in a reaction volume of 25 μ L containing 40 pmol each of the primers FIP and BIP, 5 pmol each of the outer primers F3 and B3, 20 pmol each of the loop primers FLP and BLP, 1.0 mM ...
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Loop Mediated Isothermal Amplification Assay for Rapid Detection of Common Strains of Escherichia coli
... LAMP assay was performed on an aliquot of bacterial solution without extraction of ...LAMP assay, and the re- sults were compared to cultured urine samples obtained from microbiology laboratories of the ...
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Development of a rapid loop mediated isothermal amplification assay for diagnosis and assessment of cure of Leishmania infection
... molecular assay to detect parasite DNA in clin- ical samples, therefore, we compared the threshold detec- tion limit of kDNA copies by LAMP ...LAMP assay can undoubtedly be volunteered as an efficient tool ...
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Development of a Loop Mediated Isothermal Amplification Assay for Rapid Detection of Capripoxviruses
... LAMP assay by the color change method using the metal ion-binding indicator dye HNB, which is known to bind the Mg 2⫹ ion and changes its color depending upon the pH and Mg 2⫹ concentration present in the reaction ...
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Reverse transcription, loop mediated isothermal amplification assay for the sensitive and rapid detection of H10 subtype avian influenza viruses
... The loop-mediated isothermal amplification (LAMP) technique was invented in 2000 [17] and provides a power- ful gene amplification tool with simple conditions requiring only a water ...
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A loop mediated isothermal amplification assay for the visual detection of duck circovirus
... LAMP assay, which subsequently affects the fluorescence emission of the resulting ...LAMP assay can successfully detected the DuCV field strains and showed no cross- reaction with other viral ...LAMP ...
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A loop mediated isothermal amplification assay for the detection and quantification of JC polyomavirus in cerebrospinal fluid: a diagnostic and clinical management tool and technique for progressive multifocal leukoencephalopathy
... specific amplification of the JCV genome with the LAMP assay, the plasmid DNAs of polyomaviruses (including JCV, BKV, SV40, and MPyV) were tested as templates at a concentration of ...
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Development of a loop-mediated isothermal amplification assay for rapid detection of Yersinia enterocolitica via targeting a conserved locus
... LAMP assay Cloning of the considered locus in pTZ57R vector: TA cloning procedure was done using CloneJET™ PCR cloning kit (Thermo Scientific) according to the manufacturer's ...
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Loop Mediated Isothermal Amplification for Rapid and Reliable Diagnosis of Tuberculous Meningitis
... a loop-mediated isothermal amplification assay (LAMP) was evaluated to rapidly detect and diagnose TBM infection and was compared to the performance of nested ...nested-PCR ...
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Field evaluation of malaria malachite green loop mediated isothermal amplification in health posts in Roraima state, Brazil
... MG-LAMP assay evaluated in this study can be performed using a small portable heat block and mini- centrifuge and does not require any special read-out equipment since it is a colorimetric ...
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Rapid, simple, and sensitive detection of the ompB gene of spotted fever group rickettsiae by loop mediated isothermal amplification
... LAMP assay, we tested 27 members of the order Rickettsiales along with several other bacterial ...false-positive amplification was observed with the heterogeneous strains using the SFGR LAMP assay ...
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