loop-mediated isothermal amplification (LAMP)
Development and evaluation of a Loop Mediated Isothermal Amplification (LAMP) technique for the detection of hookworm (Necator americanus) infection in fecal samples
... equipment. Loop mediated isothermal amplification technique (LAMP) is a technique developed by Notomi et ...under isothermal conditions. This nucleic acid amplification ...
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Wolbachia detection in insects through LAMP: loop mediated isothermal amplification
... Loop mediated isothermal amplification has been used to detect different organisms, such as Leishmania [22], Toxoplasma gondii [23] and also bacteria belonging to the same class as Wolbachia ...
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Development and Evaluation of Reverse Transcription Loop Mediated Isothermal Amplification Assay for Rapid and Real Time Detection of Japanese Encephalitis Virus
... As already shown in Results, the RT-LAMP assay has dem- onstrated a higher sensitivity by correctly detecting nine addi- tional positive samples with low levels of virus that were missed by RT-PCR. Further ...
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Operational Feasibility of Using Loop Mediated Isothermal Amplification for Diagnosis of Pulmonary Tuberculosis in Microscopy Centers of Developing Countries
... interpreting LAMP results independently from each ...40-min amplification period. The temperature of the amplification-and-detection device was maintained at 67°C during this ...the LAMP ...
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Diagnostic performance of a novel loop mediated isothermal amplification (LAMP) assay targeting the apicoplast genome for malaria diagnosis in a field setting in sub Saharan Africa
... processing, LAMP results would be available much faster than by microscopy and more reliably than with RDT, supporting the use of this diagnostic test for mass screening and treatment (MSAT) or focused screen- ing ...
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Field evaluation of malaria malachite green loop mediated isothermal amplification in health posts in Roraima state, Brazil
... The findings presented in this study demonstrate the accuracy of the MG-LAMP as a malaria diagnostic test in remote health posts in a malaria-endemic country. Importantly, these data demonstrate that the ...
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Evaluation of loop mediated isothermal amplification as a surveillance tool for malaria in reactive case detection moving towards elimination
... This study was part of a wider epidemiological study in northern Namibia. Details of this study are presented elsewhere [11]. Briefly, all consenting individuals liv- ing within households of cases passively detected at ...
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Field deployment of loop mediated isothermal amplification for centralized mass screening of asymptomatic malaria in Zanzibar: a pre elimination setting
... Importantly, some discrepancies were shown amongst samples screened following freezing of diluted DNA. RDT false positivity due to recently cleared infections has been well documented when detecting P. falciparum HRP2 ...
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Comparison of loop mediated isothermal amplification assay and smear microscopy with culture for the diagnostic accuracy of tuberculosis
... compared LAMP assay and smear LED fluorescent microscopy with culture as a reference standard ...both LAMP and LED fluorescent microscopy. However, the sensitivity of TB-LAMP (75%) was found to be ...
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Development of loop mediated isothermal amplification (LAMP) for simple detection of Leishmania infection
... of LAMP, which is more tolerant of PCR inhibitors, removal of the extraction process allowed a lower detection limit of 10 3 parasites/ml across all sam- ...
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Rapid Detection of Candida auris Based on Loop Mediated Isothermal Amplification (LAMP)
... reactions, LAMP reactions using a panfungal LAMP primer set (20) were run separately; amplifications were detected with templates from each of the tested species (data not ...
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Ultrasensitive loop mediated isothermal amplification (US LAMP) to detect malaria for elimination
... Primers targeting P. falciparum were modified spe- cifically to amplify 18S rRNA located in chromosome 5 and 7. The latter loci are known to be highly expressed in the blood stages of the P. falciparum [23]. By using ...
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Development of a Loop Mediated Isothermal Amplification Assay for Rapid Detection of Subgroup J Avian Leukosis Virus
... of LAMP products by alternate ...high amplification efficiency of the LAMP reaction yields large amounts of pyrophosphate ion, which leads to a white precipitate of magnesium pyrophos- phate in the ...
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A novel loop mediated isothermal amplification based test for detecting Neospora caninum DNA
... the LAMP technique for DNA amplification [17] has been shown to be versa- tile and efficient, being applied for detecting DNA from different microorganisms ...regarding LAMP being used for detect- ...
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The Development of a Loop Mediated Isothermal Amplification (LAMP) Procedure for Plague Diagnostic
... (http://primerexplorer.jp/) (Eiken Chemical Co., Ltd.). The identification of the hybridizing sites was determined through MapDraw (Lasergene ® , Version 4.01, DNASTAR Inc., Madison, WI). The critical parameters [10], ...
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Visual Detection of High Risk Human Papillomavirus Genotypes 16, 18, 45, 52, and 58 by Loop Mediated Isothermal Amplification with Hydroxynaphthol Blue Dye
... tests. Loop-mediated iso- thermal amplification (LAMP) is a nucleic acid amplification method developed by Notomi et ...gene amplification tool due to its simplicity, speed, ...
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Rapid identification of Aconitum plants based on loop mediated isothermal amplification assay
... Aconitum japonicum Thunb. subsp. subcuneatum (Nakai) Kadota, Aconitum sachalinense F. Schmidt subsp. yezoense (Nakai) Kadota, Aconitum chinense Siebold ex Paxton, Anemone flaccida, and Parasenecio delphiniifo- lius were ...
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Detection of Four Plasmodium Species by Genus and Species Specific Loop Mediated Isothermal Amplification for Clinical Diagnosis
... Loop-mediated isothermal amplification (LAMP), a novel nucleic acid amplification method, was developed for the clinical detection of four species of human malaria parasites: ...
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Subpatent malaria in a low transmission African setting: a cross sectional study using rapid diagnostic testing (RDT) and loop mediated isothermal amplification (LAMP) from Zambezi region, Namibia
... between LAMP positive and negative sub- jects in terms of care-seeking at a private versus public facility, time between onset of symptoms and care-seek- ing, or reasons for seeking or not seeking ...between ...
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Loop Mediated Isothermal Amplification Assay for Rapid Diagnosis of Malaria Infections in an Area of Endemicity in Thailand
... our LAMP assay, we first compared it to the RDTs and microscopy as a standard ...both LAMP and RDTs yielded results very similar to those of micros- ...that LAMP was prone to hav- ing greater ...
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