LTB4: leukotriene B4
Change in inflammation in out-patient COPD patients from stable phase to a subsequent exacerbation
... range; LTB4, leukotriene-B4; MCP-1, mono- cyte chemoattractant protein-1; MPO, myeloperoxidase; mRNA, messenger ribonucleic acid; TGF- β , transforming growth factor- β ; TNF- α , tumor necrosis ...
10
The Comparison of the Levels of Leukotriene B4, TGF β1, Collagen in Keloid Fibroblasts and Normal Skin Fibroblast
... [11]-[14]. Leukotriene is synthesized mainly by leukocytes and different types of cells by means of trancelullar me- tabolism including neutrophils, platelets and vascular ...cells. Leukotriene has ...
7
Relative contribution of leukotriene B4 to the neutrophil chemotactic activity produced by the resident human alveolar macrophage
... of leukotriene B4 (LTB4), a chemotactically active lipoxygenase product of arachidonic acid, we investigated the contribution of LTB4 to the total neutrophil chemotactic activity produced by ...
12
Leukotriene B4 activates intracellular calcium and augments human osteoclastogenesis
... the leukotriene biosynthesis cascade. Leukotrienes consist of leukotriene B4 (LTB4) and the cysteinyl leukotrienes: namely, leukotriene C4 (LTC4), leukotriene D4 (LTD4) and ...
12
Leukotriene B4 stimulates polymorphonuclear leukocyte adhesion to cultured vascular endothelial cells
... Our results indicated that leukotriene B4 LTB4 could effectively stimulate PMN adhesion to endothelial cell surfaces, in contrast to the sulfidopeptide leukotrienes C4, D4, and E4, and t[r] ...
5
Bacterial lipopolysaccharides prime human neutrophils for enhanced production of leukotriene B4
... neutrophils occurred in a concentration dependent manner. LPS did not result in LTB4 production in response to the chemoattractant peptide FMLP. LPS priming of neutrophils had no effect on cytosolic calcium ...
9
Acetyl glyceryl ether phosphorylcholine stimulates leukotriene B4 synthesis in human polymorphonuclear leukocytes
... indomethacin. LTB4-induced aggregation is not readily reduced by the above inhibitors of arachidonic acid ...sufficient LTB4 to account for the AGEPC response. In addition, the rate of LTB4 ...
9
Murine glomerular leukotriene B4 synthesis Manipulation by (n 6) fatty acid deprivation and cellular origin
... (LT) B4 is an important pro-inflammatory ...synthesize LTB4 when provided with exogenous substrate in a dose-dependent fashion in the presence of ionophore ...for LTB4 formation, was required for ...
7
Human alveolar macrophages have 15 lipoxygenase and generate 15(S) hydroxy 5,8,11 cis 13 trans eicosatetraenoic acid and lipoxins
... eicosatetraenoic acid (15-HETE) by physical methods. 15-LO mRNA was demonstrated in HAM by reverse transcription-polymerase chain reaction. Incubation of HAM for 3 d with interleukin 4(IL-4) before exposure to ...
9
Leukotriene B4 omega hydroxylase in human polymorphonuclear leukocytes Partial purification and identification as a cytochrome P 450
... naphthoflavone, azide, cyanide, catalase, and superoxide dismutase. Finally, isolated PMN membranes exhibited a carbon monoxide difference spectrum with a peak at 452 nm. Thus, we have partially purified the LTB4 ...
12
Decreased leukotriene B4 synthesis in smokers' alveolar macrophages in vitro
... incubations of AM with arachidonic acid and ionophore, the amounts of products formed were: LTB4, 317 +/- 56 pmol/10(6) cells and 5-HETE, 1,079 +/- 254, mean +/- SEM. No metabolites were generated under control ...
8
Neutrophil accumulation in the lung in alpha 1 antitrypsin deficiency Spontaneous release of leukotriene B4 by alveolar macrophages
... radioimmunoassay, and absorption with anti-LTB4 antibody revealed that the majority of the chemotactic activity was leukotriene B4 (LTB4), a mediator absent from normal macrophage supernates. ...
8
Effects of leukotriene B4 in the human lung Recruitment of neutrophils into the alveolar spaces without a change in protein permeability
... Leukotriene B4 (LTB4) is a major product of human alveolar macrophages and has potent chemotactic activity for neutrophils (PMN) in ...of LTB4 in the normal human lung, we instilled ...
12
Leukotriene B4 promotes neovascularization and macrophage recruitment in murine wet-type AMD models
... On the other hands, a low-affinity LTB 4 receptor, BLT2, which is ubiquitously expressed in human and mouse tissues (85) including cornea and conjunctiva (86), was not involved in las[r] ...
18
Leukotriene B4 amplifies NF κB activation in mouse macrophages by reducing SOCS1 inhibition of MyD88 expression
... B Myd88 mRNA and C MyD88 protein expression in WT and 5-LO–/– macrophages treated with Socs1 and control siRNA, as determined by real-time RT-PCR and immunoblotting, respectively; mRNA l[r] ...
13
Excessive localized leukotriene B4 levels dictate poor skin host defense in diabetic mice
... infection, leukotriene B 4 (LTB 4 ) is produced within seconds to minutes via arachidonic acid metabolism by 5-lipoxygenase (5-LO) to LTA 4 , which is further metabolized by the LTA 4 hydrolase (LTA4H) to LTB 4 ...
17
Dual inhibition of complement factor 5 and leukotriene B4 synergistically suppresses murine pemphigoid disease
... Histopatho- logical analysis and comparison of skin biopsies from mice treated with varying doses of Coversin in a preventative treatment regimen and harvested on day 12 of the antibody[r] ...
14
PU 1 is involved in the immune response to Aspergillus fumigatus through upregulating Dectin 1 expression
... Although Dectin-1 expression has been reported to be controlled by PU.1 [15] and leukotriene B4 via a GM-CSF/PU.1 axis [14] in mouse models, our results are the first to demonstrate that[r] ...
11
<p>Lipid metabolism in chronic obstructive pulmonary disease</p>
... Abbreviations: COPD, chronic obstructive pulmonary disease; CS, cigarette smoke; FAO, fatty acid oxidation; FAS, fatty acid synthesis; LXR, liver X receptor; LTB 4 , leukotriene B4; PGE [r] ...
10
Critical role of leukotriene B4 receptor signaling in mouse 3T3-L1 preadipocyte differentiation
... Methods: Mouse 3T3-L1 preadipocytes were treated with lipoxygenase (LOX) inhibitors, BLT antagonist, and small interfering RNA (siRNA) for BLT1 and BLT2 to block the LTB 4 -BLT signaling[r] ...
7