Luciferase Assay
Dual Luciferase Assay System for Rapid Assessment of Gene Expression in Saccharomyces cerevisiae
... luciferase assay. The data obtained thus far suggest that the yeast DLR assay would be an accurate and reliable reporter ...firefly luciferase reporter plasmids pDM529 and pDM531, containing ...
11
Detection of bacteriuria by luciferase assay of adenosine triphosphate
... Application of the method to 469 clinical urine specimens showed that the ATP level after treatment with Triton/apyrase was correlated to bacterial counts and that the sensitivity of the[r] ...
8
The Mdm2 Ubiquitin Ligase Enhances Transcriptional Activity of Human Papillomavirus E2
... coimmunoprecipitation assay with 293 cells that were transfected with either Mdm2 alone or Mdm2 plus GFP-tagged ...A luciferase assay was conducted in the presence of Mdm2C462A (ligase-dead mutant of ...
6
A novel loss-of-function mutation of GATA3 (p.R299Q) in a Japanese family with Hypoparathyroidism, Deafness, and Renal Dysplasia (HDR) syndrome
... the luciferase assay, 2 luciferase reporter plasmids were used: pGATA/Luc, containing the consensus GATA binding element (LR-2103, Signosis, Santa Clara, CA), and phPTH/ Luc containing the ...
6
<p>ZNF433 positively regulates the beta-catenin/TCF pathway in prostate cancer and enhances the tumorigenicity of cancer cells</p>
... Notes: (A) Luciferase assay was performed to examine the effects of ZNF433 on the reporter activity of TOPflash. Cells were treated with or without the Wnt3a protein. (B) The effects of ZnF433 knockdown on ...
9
Ectopic viral integration site-1 (evi1)-mediated repression of carbonic anhydrase iii (caiii) and its implications: role in acute myeloid leukaemia
... dual luciferase assay carried out to ascertain if EVI1 represses CAIII at the level of ...Firefly luciferase, was approximately 7 times more in Rat1neo1 cells (lane 1) than in ...3 assay was ...
5
Original Article Reduced miR-423 is related to Hirayama disease via modulating the expression of MSTN
... dual luciferase assay and immunoblotting, we identified that MSTN is a direct target of miR-423 and the target region is conserved between human and ...
8
Sweet Regulation of Melanocortin Receptor (MC1R, Mc3r, and MC4R) Transcription Activity
... Relative luciferase activity; DLA: Dual luciferase assay; MC1R: Human melanocortin 1 receptor; Mc3r: Mouse melanocortin 3 receptor; MC4R: Human melanocortin 4 receptor; Promoter model: A promoter ...
14
Luminescent multiplex viability assay for Trypanosoma brucei gambiense
... Renilla luciferase assay system (RLAS, Promega) was used to measure the RLuc activity from lysed ...RLAS assay reagent (via dispenser) in a white opaque 96-well plate (Perkin ...
9
<p>Association study of genetic variants of the <em>ANGPTL3</em> gene and susceptibility to ischemic stroke</p>
... the luciferase assay showed that promoter activity was signi fi cantly increased by 67% of plasmids containing the minor C allele compared with the major A allele in HEK293 ...
6
PHYTOCHEMICAL, CYTOTOXICITY AND DOCKING STUDIES OF SAMANEA SAMAN
... a greater percentage of methanol is added to purify the ethyl acetate fraction (10%), and a single compound was obtained. The bioactive active compound was found to be Pithecolobine having the spectral data of the IR ...
6
miR-450b-5p induced by oncogenic KRAS is required for colorectal cancer progression
... For luciferase assay, small regions containing the target sequences of mir-450b-5p in target genes 3’-UTR were generated by PCR amplification and cloned into pGL3-basic luciferase reporter plasmid ...
13
Investigation of neuronal cell type-specific gene expression of Ca2+/calmodulin-dependent protein kinase II
... Japan). Luciferase assay system, luciferase reporter pGL2- and pGL3-control vectors, pGL2- and pGL3-basic vectors, and Wizard TM Plus Minipreps DNA purification system were from Promega ...
11
Split luciferase complementation assay to detect regulated protein-protein interactions in rice protoplasts in a large-scale format
... (Co-immunoprecipitaiton) assay (Fujikawa and Kato 2007; Kato et ...SLC assay to detect a light-dependent interaction in Arabidopsis protoplasts (Kato et ...SLC assay contain the Gateway® recombinant ...
9
Wedelolactone inhibits LPS-induced pro-inflammation via NF-kappaB Pathway in RAW 264.7 cells
... and luciferase reporter assay NF-κB reporter constructs were purchased from Clon- tech Laboratories, ...reporter assay, cells were seeded into 24-well plates at a density of 5 × 10 5 cells per well ...
11
Improved osteogenic vector for non-viral gene therapy
... However, it has been demonstrated that the yield of recombinant proteins does not necessarily correlate with their mRNA levels (Barnes et al., 2004; Knappskog et al., 2007). Regarding the application of genes encoding ...
14
Serological Diagnosis of Human Herpes Simplex Virus Type 1 and 2 Infections by Luciferase Immunoprecipitation System Assay
... Antibody responses to three other HSV-1 and HSV-2 pro- teins. Previously, we demonstrated that the LIPS assay can be easily employed to evaluate whole proteomes. For example, we profiled humoral responses to the ...
6
Inhibition of melanogenesis by Gaillardia aristata flower extract
... Total RNA samples were extracted using a RNeasy Midi kit (Qiagen) and aliquot (2 μg) of total RNA was reversed transcribed using a PrimeScriptII 1st strand cDNA Synthesis kit (Takara Bio, Shiga, Japan) according to the ...
11
High expression of TFEB is associated with aggressive clinical features in colorectal cancer
... neously in the right flank of the nude mice. Tumors were measured every 4 days, and tumor volume was calculated by length × width × width/2. Meanwhile, Ki-67 and cas- pase3 were also detected by IHC assay to ...
10
Enhanced Baculovirus-Mediated Transduction of Human Cancer Cells by Tumor-Homing Peptides
... The LyP-1-VSVG, F3-VSVG, and CGKRK-VSVG fusion proteins were directed to the surface of the recombinant bac- ulovirus-infected Spodoptera frugiperda (Sf9) insect cells by the signal sequence of the major AcMNPV envelope ...
9