Luciferase Gene
Analysis of Promoter Function in Aspergillus fumigatus
... promoter-firefly luciferase fusion constructs to pyrG was generated in the pDHT/sk vector (38) amenable to Agrobacterium tumefaciens-mediated transformation ...Renilla luciferase from pH12.7 or firefly ...
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The Epstein-Barr Virus Protein BMRF1 Activates Gastrin Transcription
... the luciferase gene, 240GASLuc, as well as a series of site-directed mutants in the 240GASLuc construct, were constructed as previously described ...[CAT] gene) were con- structed as previously ...
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Combinatorial Effects of the Glucocorticoid Receptor and Krüppel-Like Transcription Factor 15 on Bovine Herpesvirus 1 Transcription and Productive Infection
... Additional studies were performed to test whether interactions between KLF15 and the GR increased the stability of either protein. Endogenous levels of the GR were not altered dramatically when Neuro-2A cells were ...
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Inherent Instability of Poliovirus Genomes Containing Two Internal Ribosome Entry Site (IRES) Elements Supports a Role for the IRES in Encapsidation
... the gene encoding ...the luciferase (nu- cleotide 1798) and VP1 (nucleotide 3034) genes to maintain the translational reading ...the luciferase-VP1 gene ...the luciferase nucleotides ...
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Nuclear Matrix Attachment Regions of Human Papillomavirus Type 16 Repress or Activate the E6 Promoter, Depending on the Physical State of the Viral DNA
... the luciferase expression vector pGL3 basic ...the luciferase gene. This second MAR was either the E5 gene including the early late intergenic region (E5-MAR, genomic positions 3536 to 4337), ...
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Novel Screening Method for Anti-Colon Cancer Drugs Using Two Sensor Cell Lines with Human β4-Galactosyltransferase 4 Gene Promoters
... To develop a novel screening method for anti-colon cancer drugs, two sensor cell lines 17.. having the luciferase gene under the control of two β4GalT4 gene promoters that [r] ...
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Receptor-targeted recombinant adenovirus conglomerates: a novel molecular conjugate vector with improved expression characteristics.
... lines gene expression significantly improved over that seen with the MCV ...short-term gene expression was significantly enhanced with use of the recMCV vectors, gene expression kinetics at the ...
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Conditionally replicating luciferase reporter phages: improved sensitivity for rapid detection and assessment of drug susceptibility of Mycobacterium tuberculosis
... A luciferase reporter phage, phAE40, has been constructed from TM4 and was previously shown to be useful for the rapid detection and drug susceptibility testing of Mycobacterium ...the luciferase activity ...
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Synthesis and in vitro bioevaluation of 1,5 Bis(5 substituted benzimidazole) alkanes as antileishmanial agents
... firefly luciferase gene, and the transfectants were maintained in medium 199 supplemented with 10% foetal calf serum, 1% penicillin (50 µ g/ml), and streptomycin (50 µ g/ml) solution (sigma) under pressure ...
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Anti-inflammatory and antioxidant effects of 2, 7-dihydroxy-4, 6-dimethoxy phenanthrene isolated from Dioscorea batatas Decne
... reporter luciferase gene connected to ARE sequence present in the promoter region of inducible antioxidant enzyme genes was increased in DDP-treated human hepatoma HepG2-ARE cells ...
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Inhibition of early steps in the lentiviral replication cycle by cathelicidin host defense peptides
... lism. Luciferase activities were determined 2 days after ...in luciferase activity of cells transduced with the lentiviral vector ...the luciferase activity of stably transduced target ...the ...
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The Expression of Circadian Clock Gene Promoters in Arabidopsis Callus.
... clock gene promoters fused to luciferase gene by Agrobacterium transformation and callus induction from seedlings and setting the system for detecting bioluminescence to determine the expression of ...
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CREB-Binding Protein and Histone Deacetylase Regulate the Transcriptional Activity of Kaposi's Sarcoma-Associated Herpesvirus Open Reading Frame 50
... We next tested the effects of several cellular proteins that associate with the KIX domain (amino acids 457 to 722) of CBP. c-Jun protein stimulated ORF50 activity in a dose-de- pendent manner (Fig. 3A), and the addition ...
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Hepatitis E Virus Genotype 1 Infection of Swine Kidney Cells In Vitro Is Inhibited at Multiple Levels
... of luciferase expression from virus replicons containing the Gaussia luciferase gene in place of the viral ORF2 or ORF3 genes demonstrated that translation of the ORF2 capsid gene of genotype ...
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Establishment of transient and stable transfection systems for Babesia ovata
... firefly luciferase gene was amplified by PCR from the plasmid pEN- T12luc using specific primers (Additional file 1: Table S1) and inserted into the EcoRV site of pBluescript plas- mid vector using an ...
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Monitoring immediate-early gene expression through firefly luciferase imaging of HRS/J hairless mice
... Background: Gene promoters fused to the firefly luciferase gene (luc) are useful for examining gene regulation in live transgenic mice and they provide unique views of functioning ...of ...
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Proof of concept studies for siRNA delivery by non-ionic surfactant vesicles : in vitro and in vivo evaluation of protein knockdown
... uptake, luciferase gene silencing mediated by siLUC loaded in the CN formulation was ...evaluated. Luciferase is a bioluminescence producing enzyme widely used for monitoring siRNA delivering ...
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In vivo monitoring of fetoplacental Vegfr2 gene activity in a murine pregnancy model using a Vegfr2-luc reporter gene and bioluminescent imaging
... Angiogenesis is a developmental process that becomes relatively quiescent in adulthood except during certain conditions such as tumor development [30], wound heal- ing [31] and corpus luteum development [19]. However, ...
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Functional studies on the rotavirus non structural proteins NSP5 and NSP6
... Figure 5.7 Competition filter binding assay of unlabelled rotavirus gene 197 segment 7 ssRNA or Luciferase ssRNA competing with 32P-Iabelled rotavirus gene segment 7 ssRNA for binding by[r] ...
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A redesigned CRISPR/Cas9 system for marker free genome editing in Plasmodium falciparum
... resistance gene (AmpR) and a ColEl replication origin ...regular gene editing in Pf remains ...basic gene editing function of this Cas9-mediated suicide-rescue ...
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