Lymphoblastoid cell line
Genetic and genomic stability across lymphoblastoid cell line expansions
... In total, we identified 22 unique CNV regions across all 129 samples (Additional file 3: Table S2); however, 3 of these were immunoglobulin (IG) regions that have been previously implicated in cell culture ...
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Induction of selective cytotoxicity and apoptosis in human T4 lymphoblastoid cell line (CEMss) by boesenbergin a isolated from boesenbergia rotunda rhizomes involves mitochondrial pathway, activation of caspase 3 and G2/M phase cell cycle arrest
... remaining media. Ten microliters of fluorescent dyes containing AO (10 μg/ml) and PI (10 μg/ml) were added into the cellular pellet at equal volumes of each. The freshly stained cell suspension was dropped into a ...
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The prototypical Epstein-Barr virus-transformed lymphoblastoid cell line IB4 is an unusual variant containing integrated but no episomal viral DNA.
... We have also shown previously that cells with multiple episomes show multiple tracks of viral RNA synthesis, whereas a cell line that has only integrated viral DNA Namalwa shows only one[r] ...
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Characterization of a Marek's disease virus BamHI-L-specific cDNA clone obtained from a Marek's disease lymphoblastoid cell line.
... 1191 Downloaded from http://jvi.asm.org/ on November 9, 2019 by guest Two Marek's disease MD virus BamHI-L-specific cDNA clones were isolated from a cDNA library constructed from polyA+ [r] ...
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Epstein-Barr virus DNA recombines via latent origin of replication with the human genome in the lymphoblastoid cell line RGN1.
... It seems noteworthy that the sequence of 20-bp repeats from the human distal part of the pJE clone displays a high level of identity with some markers with variable numbers of terminal r[r] ...
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Differences among human immunodeficiency virus strains in their capacities to induce cytolysis or persistent infection of a lymphoblastoid cell line immortalized by Epstein-Barr virus.
... Some dilutions of the first strain examined, human T-cell lymphotropic virus type III B, which is derived from a pool of patient isolates propagated in H9 cells, caused transient cytopat[r] ...
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Replication of Epstein-Barr virus: ultrastructural and immunofluorescent studies of P3HR1-superinfected Raji cells.
... The human lymphoblastoid cell line Raji, derived from a Burkitt's lymphoma, contains the EBV genome in a repressed state; no early antigens EA or viral capsid antigens VCA are produced, [r] ...
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Interchromosomal Gene Conversion at an Endogenous Human Cell Locus
... To examine the relationship between gene conversion and reciprocal exchange at an endogenous chromosomal locus, we developed a reversion assay in a thymidine kinase deficient mutant, TX545, derived from the human ...
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T lymphocyte clones responsive to Shigella flexneri
... Proliferation of clone MC12 in response to EDL and irradiated PBM or a B-lymphoblastoid cell line.. a Clone MC12 2.5 x 104 cells per well with irradiated donor X PBM 5 x 104 cells per we[r] ...
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Marek's Disease Virus Type 2 (MDV-2)-Encoded MicroRNAs Show No Sequence Conservation with Those Encoded by MDV-1
... MSB-1 lymphoblastoid cell line is coinfected with MDV-1 and MDV-2. Cocultivation of MSB-1 cells on CEF produced MDV-specific plaques 6 to 7 days after incubation. The specific identity of viral ...
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Latent transcripts of Marek's disease virus are clustered in the short and long repeat regions.
... PolyA+ RNAs extracted from MDV-induced kidney lymphoma cells and from MKT-1 cells, a nonproducing lymphoblastoid cell line derived from a tumor induced by MDV, were electrophoretically s[r] ...
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Characterization of cloned chicken anemia virus DNA that contains all elements for the infectious replication cycle.
... VAN Circular double-stranded replication intermediates were identified in low-molecular-weight DNA of cells of the avian leukemia virus-induced lymphoblastoid cell line 1104-X-5 infected[r] ...
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T lymphocyte cloning from rejected human kidney allografts Growth frequency and functional/phenotypic analysis
... Abbreviations used in this paper: A-BLCL, autologous BLCL; BRMP, Biological Response Modifiers Program; BLCL, B lymphoblastoid cell line; CFC, colony-forming cells; CM, culture medium; D[r] ...
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Herpesvirus saimiri-induced lymphoblastoid rabbit cell line: growth characteristics, virus persistence, and oncogenic properties.
... TORRISI3 Received 14 February 1985/Accepted 20 May 1985 A nonproducer lymphoblastoid cell line 7710 containing the herpesvirus saimiri HVS genome was established from the HVS-positive sp[r] ...
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Origin of the minor glycoproteins of murine leukemia viruses.
... DISCUSSION We have investigated the origin of minor glycoproteins from R-MuLV and membranes of a thymic murine leukemia-derived lymphoblastoid cell line which are chemically and antigeni[r] ...
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Negative modulation of Epstein-Barr virus episomes by a human B-cell growth factor.
... As demonstrated by the slot blot technique, DNA derived from Raji cells as well as from the lymphoblastoid cell line maintained in the presence of BCGF-12kD exhibited a significant reduc[r] ...
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Integrative bioinformatics analysis characterizing the role of EDC3 in mRNA decay and its association to intellectual disability
... from lymphoblastoid cell line samples of patients’ cells carrying the EDC3 variant as well as from an in vitro EDC3 loss-of-function neuronal model based on siRNA knockdown we identified several ...
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Isolation and characterization of cDNA clones corresponding to transcripts from the BamHI H and F regions of the Epstein-Barr virus genome.
... To characterize transcripts that are encoded in this region, polyA+ RNA from the EBV-transformed lymphoblastoid cell line JY was isolated, and this RNA was used to generate a cDNA librar[r] ...
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Use of a BJAB Derived Cell Line for Isolation of Human Herpesvirus 8
... to the 8th to 10th day of culture. Interestingly, double-staining studies dem- onstrated that 30 to 80% of the BBF cells expressing PF8 coex- pressed vIL-6 (Fig. 3H) and that cells expressing only vIL-6 were very rarely ...
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Take it personal! Genetic differences in G protein-coupled receptors as studied with label-free technology
... adherent cell lines [24, 31, ...monocyte cell line, using an optics-based assay [27, ...monocyte cell lines (THP-1 and U937), neutrophils and primary normal peripheral blood monocytes (PBMCs) ...
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