Major Late Promoter
The mammalian upstream element factor recognizes two sites in the adenovirus type 2 IVa2-major late promoter intergenic region and stimulates both promoters.
... 7 The Mammalian Upstream Element Factor Recognizes Two Sites in the Adenovirus Type 2 IVa2-Major Late Promoter Intergenic Region and Stimulates Both Promoters VINCENT MONCOLLIN, ANNE KEM[r] ...
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Functional Analysis of the CAAT Box in the Major Late Promoter of the Subgroup C Human Adenoviruses
... the major late promoter (MLP) of adenoviruses from a wide variety of host species show that an inverted CAAT box is among the most highly conserved transcription elements found in the putative ...
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Properties of the adenovirus IVa2 gene product, an effector of late-phase-dependent activation of the major late promoter.
... adenovirus major late promoter is strongly activated after the onset of viral DNA ...the major late promoter start site have previously been shown to be essential for this ...a ...
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Activation of the adenovirus major late promoter by transcription factors MAZ and Sp1.
... 5 major late promoter have been identified by DNase I protection ...the promoter, both MAZ and Sp1 interact with GC-rich sequences flanking the TATA ...the major late ...
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The initiator element of the adenovirus major late promoter has an important role in transcription initiation in vivo.
... adenovirus major late promoter (MLP) can be analyzed genetically in its correct location, despite its essential role in the viral life ...the promoter, and if its function was affected by ...
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Adeno-Associated Virus Rep Protein-Mediated Inhibition of Transcription of the Adenovirus Major Late Promoter In Vitro
... Rep78/68 bind to the AdMLP. The results described above demonstrate that nonspecific DNA in the form of poly(dI-dC) or poly(dG-dC) did not alleviate Rep68-mediated inhibition of tran- scription. To determine whether Rep ...
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The Adenovirus L4 33-Kilodalton Protein Binds to Intragenic Sequences of the Major Late Promoter Required for Late Phase-Specific Stimulation of Transcription
... FIG. 2. Purification of DEF-A. (A) Summary of the purification scheme. (B) Electrophoretic mobility shift assay of fractions recovered from the Superdex S-200 column. The positions of the infected-cell-specific complexes ...
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A unique mitigator sequence determines the species specificity of the major late promoter in adenovirus type 12 DNA.
... Complex formation of the Adl2 MLP mitigator element and of related nucleotide sequences with proteins in nuclear extracts from hamster BHK21 or human HeLa cells.. Nucleotide sequences of[r] ...
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Stimulation of the adenovirus major late promoter in vitro by transcription factor USF is enhanced by the adenovirus DNA binding protein.
... We and others reported previously that DBP is able to enhance the binding of the cellular transcription factor nuclear factor I NFI to its recognition site in the adenovirus auxiliary or[r] ...
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The adenovirus type 2 DNA-binding protein interacts with the major late promoter attenuated RNA.
... 1134 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest The adenovirus 72-kilodalton DNA-binding protein DBP binds to the attenuated RNA derived from the viral major late [r] ...
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The upstream factor-binding site is not essential for activation of transcription from the adenovirus major late promoter.
... Previous results 42 have suggested that the USF-binding site plays a role in the expression of the divergent IVa2 transcription unit whose start site is located 210 bases upstream from t[r] ...
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High-level transcription from the adenovirus major late promoter requires downstream binding sites for late-phase-specific factors.
... To measure the significance of these binding sites for transcription during the late phase of infection, we constructed and analyzed nondefective adenovirus recombinants in which MLP reg[r] ...
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The downstream regulatory sequence of the adenovirus type 2 major late promoter is functionally redundant.
... However, the +75/+125 deletion severely inhibited the stimulation of transcription observed in late-phase infectedcell extracts, reducing the ratio of infected- to uninfected5685 Downloa[r] ...
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Positive and negative control sequences within the distal domain of the adenovirus IVa2 promoter overlap with the major late promoter.
... When plasmid pAd242 that contains the IVa2 promoter and 200 bp of downstream transcribed sequences, the MLP and its RNA start site, and 33 bp of downstream sequences were transcribed in [r] ...
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Modification of an adenovirus major late promoter-binding factor during poliovirus infection.
... Since virus-induced modification of the UPE-USF complex parallels the in vivo inhibition of host cell RNA synthesis, the transcriptional activity of nuclear extracts from poliovirus-infe[r] ...
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The product of the adenovirus intermediate gene IVa2 is a transcriptional activator of the major late promoter.
... 4450-4457 0022-538X/94/$04.00+0 Copyright C 1994, American Society for Microbiology The Product of the Adenovirus Intermediate Gene IVa2 Is a Transcriptional Activator of the Major Late [r] ...
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CTGC motifs within the HIV core promoter specify Tat responsive pre initiation complexes
... core promoter binding complexes are highly diverse [6,30-35], together with recent advances in the experimental detection of endogenous pre-initiation complexes [36], we have revisited TASHET ...Adenovirus ...
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Expression systems for adenovirus late proteins
... Adenovirus DNA replication facilitates binding of the MLTFIUSF transcription factor to the viral major late promoter within infected cells.. Electrophoretic transfer of proteins from pol[r] ...
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Cloning and characterization of a novel cellular protein, TDP-43, that binds to human immunodeficiency virus type 1 TAR DNA sequence motifs.
... Human immunodeficiency virus type 1 (HIV-1) gene expression is modulated by both viral and cellular factors. A regulatory element in the HIV-1 long terminal repeat known as TAR, which extends from nucleotides 2 18 to 1 ...
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Transcription unit mapping in adenovirus: regions of termination.
... With this assay, we proceeded to examine labeled nuclear RNA early after infection for evidence of specific termination sites within the major late promoter region.. The analysis of RNA [r] ...
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