MALDI-mass spectrometry imaging
3D MALDI Mass Spectrometry Imaging of a Single Cell: Spatial Mapping of Lipids in the Embryonic Development of Zebrafish
... (MALDI)-mass spectrometry imaging (MSI) to map and visualize the three-dimensional spatial distribution of phospholipid classes, phosphatidylcholine (PC), phosphatidylethanolamines (PE), and ...
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Examination of tumour tissues by direct MALDI mass spectrometry imaging and profiling
... by MALDI-MSI analysis of non-treated and AQ4N-treated xenograft tissue ...by MALDI-MSI analysis of intact proteins directly from tissue sections is in most cases low and results in the detection of mainly ...
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Localization of tamoxifen in human breast cancer tumors by MALDI mass spectrometry imaging
... in MALDI-MSI experiments. Utilizing the superior mass resolution and accuracy of the Orbitrap mass analyzer, full scan mode was chosen for data acquisition in imaging mode rather than tandem ...
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Molecular imaging of drug transit through the blood-brain barrier with MALDI mass spectrometry imaging
... The permeability of BKM120 through the BBB in mouse brain can be directly visualized using MALDI MSI. For the images shown in Figure 2, mice were dosed with BKM120 by oral gavage and sacrificed after 4 hours. ...
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Analysis of Lipids in Kidney Tissue Using High Resolution MALDI Mass Spectrometry Imaging
... Mass Spectrometry Imaging (MSI) has become an indispensable tool for studying the spatial distribution of molecules within a diverse range of complex biological ...modern mass spectrometers ...
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Multimodal FTIR Microscopy-guided Acquisition and Interpretation of MALDI Mass Spectrometry Imaging Data
... restrictive mass feature identification Integration of the molecular information provided by MALDI-MSI and tissue anatomical structures that can be resolved by microscopy is mandatory for its clinical ...
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High-resolution MALDI mass spectrometry imaging of gallotannins and monoterpene glucosides in the root of Paeonia lactiflora
... cellular imaging, subtle differences in vessels and xylem fibres were visualized with high-quality composite ion images (marked with white line in ...Next, mass spectral data of 6 regions of interest, ...
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Investigation of Cancerous Tissues by MALDI Mass Spectrometry Imaging - Imaging of proteolytic activity in frozen tissue and standardised on-tissue digestion
... using MALDI MSI adds another group of biological targets to the wide range of MALDI MS accessible target ...Here MALDI-FT-ICR MS data gives superior results over MALDI-TOF MS data, with ...
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3D MALDI Mass Spectrometry Imaging of a Single Cell: Spatial Mapping of Lipids in the Embryonic Development of Zebrafish
... 2D MALDI-MSI data from a tissue section is often used to estimate lipid molar compositions, ignoring vol- umetric contribution 29 ...2D MALDI-MSI dataset, using the matrix ion signal at each voxel ...
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Investigation of the Chemical Interface in the Soybean–Aphid and Rice–Bacteria Interactions Using MALDI-Mass Spectrometry Imaging
... Most MSI applications for plant materials are made through direct interrogation on plant surfaces or thin sections obtained by cryosectioning. 4,17 Unfortunately, neither method is applicable for visualization of the ...
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Toward Higher Sensitivity in Quantitative MALDI Imaging Mass Spectrometry of CNS Drugs Using a Nonpolar Matrix
... in MALDI mass spectrometry imaging (MSI), the negative impact of which on precision and accuracy in quantitative MALDI-MSI can be reduced to some extent by applying isotope internal ...
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Imaging and mapping of mouse bone using MALDI-imaging mass spectrometry
... desorption/ionization-imaging mass spectrometry (MALDI-IMS) is an advanced method used globally to analyze the distribution of biomolecules on tissue cryosections without any ...whether ...
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On-Tissue Protein Identification and Imaging by MALDI-Ion Mobility Mass Spectrometry
... by mass spectrometry [43– 46] ...the mass spectrum and allows the identifi- cation of trends and chemical families, although, IM does not enhance sensitivity since it does not modify the ionization ...
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A Two-Step Soft Segmentation Procedure for MALDI Imaging Mass Spectrometry Data
... The LDA model has had success in text processing, but its applications are not limited to information retrieval problems. In this work, we have applied LDA to the clustering of the spectra. Note that in ...
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Examining the role of ganglioside homeostasis in neurodegeneration and aging using MALDI imaging mass spectrometry
... and MALDI IMS images from the ET-1 alone group (Fig ...and MALDI IMS do not necessarily measure the same thing, especially with respect to detection of ...while MALDI IMS measures the signal ...
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Histology-Compatible MALDI Mass Spectrometry Based Imaging of Neuronal Lipids for Subsequent Immunofluorescent Staining.
... intensity of all matrix derived signals in both positive and negative polarities (Figure 1). Effect of a) laser pulse energy (10 laser shots) and b) number of laser shots (at threshold 2[r] ...
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Region-specific bioconversion of dynorphin neuropeptide detected by in situ histochemistry and MALDI imaging mass spectrometry
... (A) MALDI imaging MS of endogenous dynorphins in experimental Parkinson’s disease ...with MALDI imaging MS, where the enzyme inhibitor is first added to one side of a normal control brain ...
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Mass Spectrometry Imaging in Nanomedicine
... from MALDI with that from MRI and immunohistochemistry (hematoxylin and eosin staining) using PAXgene fixation provided 3D information about the kidney post-infection, an approach that could be applied to other ...
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Ganglioside Detection from Formalin Fixed Human Brain Tissue Utilizing MALDI Imaging Mass Spectrometry
... studies. Imaging of formalin fixed brain tissue in particular will allow for further studies of clinical brain samples diagnosed with a neurodegenerative disease that have been kept in storage for extended periods ...
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MALDI imaging mass spectrometry of N-linked glycans on formalin-fixed paraffin-embedded murine kidney
... of mass spectrom- etry (MS)-based methods available for interrogating clin- ical tissue samples for variations in glycosylation ...(MALDI) imaging, provides a unique solution to these issues [ 9 ]. ...
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