maltose-binding protein
A maltose-binding protein/adeno-associated virus Rep68 fusion protein has DNA-RNA helicase and ATPase activities.
... Rep68 protein produced in Escherichia coli as a fusion protein with maltose-binding protein (MBP-Rep68 D ) has previously been shown to possess DNA-DNA helicase activity, as does the ...
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Strep-tag ii mutant maltose-binding protein for reagentless fluorescence sensing
... Abstract: Maltose-binding protein (MBP) is a periplasmic binding protein found in Gram negative ...in maltose transport and bacterial chemotaxis; it binds to maltose and ...
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Construction of a strep-tag II mutant maltose binding protein for reagentless fluorescence sensing
... Maltose binding protein (MBP) changes its conformational structure upon its ligand ...recombinant protein fusion was then purified by affinity purification using Strep-Tactin ...sense ...
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Overexpression and purification of U24 from human herpesvirus type-6 in E. coli: unconventional use of oxidizing environments with a maltose binding protein-hexahistine dual tag to enhance membrane protein yield
... brane protein overexpression ...membrane protein yield, increasing our mechanistic understanding of prokaryotic systems of membrane protein translation and folding is clearly ...membrane ...
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Antigenicity of Recombinant Maltose Binding Protein-Mycobacterium avium subsp. paratuberculosis Fusion Proteins with and without Factor Xa Cleaving
... J ohne’s disease (JD) in ruminants is a chronic infection of the intestines caused by Mycobacterium avium subsp. paratubercu- losis. Economic losses arise due to culling, reduced production of milk and wool, and ...
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Adeno-associated virus DNA replication in vitro: activation by a maltose binding protein/Rep 68 fusion protein.
... The results of the in vitro assay using an extract from uninfected HeLa cells supplemented with Rep 68MBP are similar to both the results of an in vitro assay using an extract from AAV/A[r] ...
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In vivo polyester immobilized sortase for tagless protein purification
... recombinant protein production and purification techniques are often complicated, involving multiple chromatography steps and specialized equipment and ...target protein led to a simple protein ...
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Late onset X linked sideroblastic anemia Missense mutations in the erythroid delta aminolevulinate synthase (ALAS2) gene in two pyridoxine responsive patients initially diagnosed with acquired refractory anemia and ringed sideroblasts
... Abbreviations used in this paper: ALAS, aminolevulinate synthase; ALAS2, erythroid-specific 6-ALAS gene; Hb, hemoglobin; MBP, maltose-binding protein; MCV, mean corpuscular volume; nt, n[r] ...
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Characterization of a tryptophanyl-tRNA synthetase Urzyme and its intramolecular complementation establishes a model to study the catalytic mechanism and evolution of the contemporary Class I aminoacyl-tRNA synthetases
... With the implementation of pMAL-c2 expression system, I have successfully solubilized the redesigned TrpRS Urzyme. As previously discussed, the pET42a (+) plasmid carrying TrpRS Urzymes tends to be unstable and lost ...
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Large Protein Folding and Dynamics Studied by Advanced Hydrogen Exchange Methods
... large protein folding. Regardless of protein size, research has generally lacked the structural tools with necessary temporal resolution to provide mechanistic insight into the ...large protein ...
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Sequence requirements for stable binding and function of Rep68 on the adeno-associated virus type 2 inverted terminal repeats.
... This report demonstrates the ability of both recombinant fusion maltose-binding protein MBP-Rep68A produced in Escherichia coli and wild-type wt Rep68 to bind to a linear truncated form [r] ...
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Enhancement of solubility in Escherichia coli and purification of an aminotransferase from Sphingopyxis sp. MTA144 for deamination of hydrolyzed fumonisin B1
... Likewise, protein production but not solubility correlated with the IPTG concentration in ...effect. Maltose-binding protein, used as an N-terminal fusion partner, promoted solubility at 30°C ...
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Impact of Human Immunodeficiency Virus Type 1 gp41 Amino Acid Substitutions Selected during Enfuvirtide Treatment on gp41 Binding and Antiviral Potency of Enfuvirtide In Vitro
... a maltose binding protein chimera containing a portion of the gp41 ectodomain and measuring their binding affinity to fluorescein-labeled ...in binding affinity for the mutant gp41 ...
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Investigation of a novel intein based Escherichia coli expression system for human methylmalonyl CoA mutase : a thesis presented to Massey University in partial fulfilment of the requirements for the degree of Master of Science in Biochemistry
... Based on the results of previous expression systems, four novel expression vectors were developed utilising the maltose binding protein and thioredoxin as solubility tags.. lt was hoped [r] ...
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The herpes simplex virus 1 UL15 gene encodes two proteins and is required for cleavage of genomic viral DNA.
... We report that i a polyclonal rabbit antiserum generated against a chimeric protein consisting of the bacterial maltose-binding protein fused in frame to the majority of sequences contai[r] ...
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The Genome of Swinepox Virus
... resistance protein, interleukin-18 binding protein, IFN- ␣ /  binding protein, extracellular enveloped virus host range protein, dUTPase, hydrox- ysteroid dehydrogenase, ...
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Functional analysis of a murine monoclonal antibody against the repetitive region of the fibronectin binding adhesins fibronectin binding protein A and fibronectin binding protein B from Staphylococcus aureus
... ligand binding involves not merely competition with Fn, but also a conformational change in the repeat that results in pre- venting Fn from binding its own ...antibody binding shifts adhesin repeats ...
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Biological roles of CCAAT/Enhancer-binding protein delta during inflammation
... TBI is also known to increase the expression of amyloid precursor protein (APP) [70]. Many studies have reported that TBI-induced Aβ accumulation can further contribute to AD formation [71,72], but the detailed ...
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No effect of protein binding ratio of levofloxacin in hemodialysis patients
... and protein binding ratio of ...between protein concentration and the protein binding ratio of LVFX in vitro and in vivo, the effect of ...
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<p>Molecular dynamics study to improve the substrate adsorption of <em>Saccharomycopsis fibuligera</em> R64 alpha-amylase by designing a new surface binding site</p>
... supporting hydrophobic forces to the stability of SBS. The RMSD of the substrate in MT3 showed that it could bind to the SBS until 55 ns. Time evolution snapshot every 20 ns (Figure 5) revealed that substrate ...
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