Markers and genotyping
Efficient Imputation of Missing Markers in Low-Coverage Genotyping-by-Sequencing Data from Multiparental Crosses
... of markers, which is one of the main features of ...for markers and genotyping several replicates of founders are simple ways to improve the overall data ...
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Sequence Generation and Genotyping of 15 Autosomal STR Markers Using Next Generation Sequencing
... ultimate genotyping method to overcome the limitations of capillary electrophoresis (CE)- based short tandem repeat (STR) analysis, such as the limited number of STR loci that can be measured simultaneously using ...
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Reliable identification of women with CIN3+ using hrHPV genotyping and methylation markers in a cytology-screened referral population
... Statistical analysis Results of cytology, hrHPV testing and methylation testing were studied for their performance in the detection of CIN3+ lesions. For analysis cytological outcome was classified as ASC-US/LSIL (ASC-US ...
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Development of amplicon deep sequencing markers and data analysis pipeline for genotyping multi-clonal malaria infections
... The power of high sequencing coverage was shown for example in a study assessing the subclonal diversity in car- cinomas [27]. Minority variants with a frequency of 1:10′ 000 were detected with a sequence depth of ...
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Genome-wide development and use of microsatellite markers for large-scale genotyping applications in foxtail millet [Setaria italica (L.)]
... 159 markers were validated successfully in foxtail millet ...microsatellite markers developed here showed their utility in high ampli- fication and polymorphic potential, cross-genera transferability and in ...
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Characterization of Ancestral Mycobacterium tuberculosis by Multiple Genetic Markers and Proposal of Genotyping Strategy
... To our knowledge, this is the first characterization of this genogroup by multiple genetic markers based on a fairly large sample size. In this genogroup, all spoligotypes were characterized by the absence of ...
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Highly Discriminatory Variable Number Tandem Repeat Markers for Genotyping of Trichophyton interdigitale Strains
... VNTR markers presented in this work showed high levels of discrimination and specificity for interstrain differ- entiation of ...three markers for 60 strains (25), to ...microsatellite markers were ...
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Development of user-friendly markers for Black Root Rot (BRR) resistance in Burley tobacco through Genotyping by sequencing (GBS)
... These undesirable traits could be caused by deletion of genes near RBRR1 as a consequence of linkage drag, or by pleiotropic effects of RBRR1 Closely linked markers should be very helpful to identify the breakage ...
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Indel and single nucleotide variations of zeins generate unique 2D-zein patterns and molecular markers useful in maize (Zea mays) genotyping
... A: Total ethanol extracts from mature seeds of maize varieties and the W64a inbred were subjected to isoelectrofocusing in the first dimension and then to SDS-PAGE in the second dimension. Loading was about 130 μg for ...
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Applicability of anatid and galliform microsatellite markers to the genetic diversity studies of domestic geese (Anser anser domesticus) through the genotyping of the endangered zatorska breed
... Unfortunately, those authors have not worked out many species within the Anatidae family, but it has been shown that the DNA - DNA TmH among this family is certainly lower than 9.8. Any data for the genus Anser are not ...
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A post labeling method for multiplexed and multicolored genotyping analysis of SSR, indel and SNP markers in single tube with bar coded split tag (BStag)
... Discussion The designed BStag was short enough to be attached as a tag for a sequence-specific primer of interest, and was appropriate for both single and multiplexed post-PCR labeling analysis. Post-PCR labeling was ...
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Multilocus Genotyping Reveals New Molecular Markers for Differentiating Distinct Genetic Lineages among “Candidatus Phytoplasma Solani” Strains Associated with Grapevine Bois Noir
... Molecular markers of genetic diversity among grapevine-affecting phytoplasmas set a solid foundation to improve knowledge of BN ...molecular markers useful for fine differentiation of CaPsol genetic ...
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A High-Density Linkage Map of the Ancestral Diploid Strawberry, Fragaria iinumae, Constructed with Single Nucleotide Polymorphism Markers from the IStraw90 Array and Genotyping by Sequencing
... markers, the Fii map will constitute a key resource for the anchoring of an F. iinumae genome assembly. Com- parisons with the F. iinumae genome will help to reveal remaining errors in the F. vesca reference ...
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Genotyping Error Detection Through Tightly Linked Markers
... of genotyping errors is an important issue in mapping complex disease ...multiple markers in a candidate region in genetic studies, the potential benefit of jointly analyzing multiple markers to ...
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Genotyping in the MHC locus: potential for defining predictive markers in sarcoidosis
... the genotyping findings. Since genotyping data for these patient groups were not obtained and since the patient cohorts differ, however, no direct comparison can be made to resolve this apparent ...
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Genotyping of Toxoplasma gondii Isolates with 15 Microsatellite Markers in a Single Multiplex PCR Assay
... Faculte ´ de Me ´decine, Universite ´ de Limoges, Limoges, 87025, France 2 Received 7 June 2010/Returned for modification 28 July 2010/Accepted 17 September 2010 We developed an easy-to-use method for genotyping ...
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Linkage mapping in prairie cordgrass (Spartina pectinata Link) using genotyping-by-sequencing markers
... quality markers that exhibited statistical ...order markers on the ...GBS markers and homology between linkage groups was ...utilizes markers that are polymorphic in both parents to determine ...
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Genotyping of Plasmodium falciparum using antigenic polymorphic markers and to study anti malarial drug resistance markers in malaria endemic areas of Bangladesh
... Discussion The findings on genetic diversity based on the poly- morphic genes msp-1 and glurp of P. falciparum isolates from a malaria-endemic region of Bangladesh, comple- menting earlier work in a different location ...
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Molecular Genotyping of Candida parapsilosis Group I Clinical Isolates by Analysis of Polymorphic Microsatellite Markers
... parapsilosis group I isolates. PCR primer sets that flank the microsatellite repeats for seven loci were designed. Following amplification by PCR, the size of each amplification product was determined automatically by ...
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Pungency Genotyping Of Commercially Cultivated Chilli Pepper Capsicum Species Using Map1 And Csy 1 Molecular Markers
... chinense variety Kotpar, using pungency genotyping molecular markers viz., Pun 1 allele specific primer MAP1 and Capsaicin synthase gene Csy1 primer. Pungency genotyping in chilli Fig.2. A: DNA ...
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