MBP-TCLC, myelin basic protein-specific T cell line cells
Decreased dependence of myelin basic protein reactive T cells on CD28 mediated costimulation in multiple sclerosis patients A marker of activated/memory T cells
... inhibit MBP-specific proliferation in the healthy con- trols, and conversely have no effect on proliferation of MBP- reactive T cells in MS patients if these cells play a role in ...
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Immunodominance of a low affinity major histocompatibility complex binding myelin basic protein epitope (residues 111 129) in HLA DR4 (B1*0401) subjects is associated with a restricted T cell receptor repertoire
... to MBP is present, both at the level of fine epitope recognition (8–12) and T cell receptor us- age (43–45), although an association of the immunodominant central epitope of MBP(87–106, 81–99) ...
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Myelin basic protein specific T lymphocyte repertoire in multiple sclerosis Complexity of the response and dominance of nested epitopes due to recruitment of multiple T cell clones
... of MBP-specific T cell responses, a minority of MS patients were found to exhibit a more restricted response with respect to their TCL epitope ...
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Krishnamoorthy, Gurumoorthy (2007): Devic mouse: a spontaneous double-transgenic mouse model of human opticospinal multiple sclerosis and autoimmune T- B cell cooperation. Dissertation, LMU München: Fakultät für Biologie
... by T cells at intermediate affinity leads to positive ...These T cells are exported to the periphery. Self-reactive T cells, which recognize the antigenic epitopes shared by self ...
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Autoimmunity-related demyelination in infection by Japanese encephalitis virus
... neuronal cells and causes an inflammatory response after invasion of the parenchyma of the ...that myelin-specific antibodies appeared in sera, particularly in those mice with evident ...Meanwhile, ...
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Homologies between T cell receptor junctional sequences unique to multiple sclerosis and T cells mediating experimental allergic encephalomyelitis
... isolate T cells reactive to myelin basic protein (MBP) in patients with multiple sclerosis ...These T cell clones are activated in vivo, and are not found in ...
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Induction of circulating myelin basic protein and proteolipid protein specific transforming growth factor beta1 secreting Th3 T cells by oral administration of myelin in multiple sclerosis patients
... regulatory T cells that mediate ac- tive suppression by secreting the cytokine TGF- b ...presumed T cell–mediated Th1 type au- toimmune ...oral myelin treatment, containing both ...
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Kyratsous, Nikolaos (2016): Calcium signals in encephalitogenic T cells on their way into central nervous system tissues: an in vivo imaging study. Dissertation, LMU München: Medizinische Fakultät
... the cells and lead to T cell ...detect T cell activation induced by antigen recognition in vivo. During T cell maturation in the spleen, both myelin basic ...
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Reactivity to myelin antigens in multiple sclerosis Peripheral blood lymphocytes respond predominantly to myelin oligodendrocyte glycoprotein
... Although T cell responses to the quantitatively major myelin proteins, myelin basic protein (MBP) and proteolipid protein (PLP), are likely to be of importance in ...
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Experimental allergic encephalomyelitis in cynomolgus monkeys Quantitation of T cell responses in peripheral blood
... Proliferative T lymphocyte responses to BH, to myelin basic protein (MBP), but not to proteolipid protein, were detected in peripheral blood mononuclear cells (PBMC) of ...
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Autoimmune-induced preferential depletion of myelin-associated glycoprotein (MAG) is genetically regulated in relapsing EAE (B6 × SJL) F1 mice
... different, specific underlying immune regulatory mecha- nisms have been proposed in MS ...godendrocyte specific damage is depletion of myelin asso- ciated glycoprotein (MAG), which is a type I ...
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Monitoring chemically-induced myelin damages in the central nervous system via surface-recorded cerebellar auditory evoked potentials and radioimmunoassay of myelin basic protein in the cerebrospinal fluid
... the myelin sheath alone, or combined damage to both myelin and its related myelinating cells, and the latter occurs secondary to degeneration of the axon (namely Wallerian degeneration, a process ...
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Immunodominant fragments of myelin basic protein initiate T cell-dependent pain
... Neuropathology, immunohistochemistry, and microscopy Plastic-embedded transverse nerve sections (0.75 μm each) were used for neuropathologic evaluation. Sciatic nerves were isolated and placed in 2.5% glutaraldehyde in ...
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Immune reactivity in the nervous system: modulation of T lymphocyte activation by glial cells
... Astrocytes present myelin basic protein to encephalitogenic T cell lines.. prostaglandin E and an interleukin-1 like-factor by astrocytes and C6 glioma cells.[r] ...
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Original Article Combination of Salidroside and hyperbaric oxygen improves leukoaraiosis through P13K/Akt signaling
... Frozen sections were sequentially treated with 3% H 2 O 2 -methanol at 4°C for 10 min, 0.1% Triton X-100/0.1% sodium citrate solution at 4°C for 2 min, TUNEL reaction mixture at 37°C for 60 min , converter-POD at 37°C ...
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Design of Linear and Cyclic Mutant Analogues of Dirucotide Peptide (MBP82–98) against Multiple Sclerosis: Conformational and Binding Studies to MHC Class II
... B cell lines HTC-Lan (DRB1*1501, DRB5*0101) or BSM (DRB1*0401) were used for isolating MHC class II ...BSM cell pellets were lyzed by Nonidet P-40, and HLA-DR2/DR4 were isolated from homogenates by affinity ...
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Neuromyelitis optica study model based on chronic infusion of autoantibodies in rat cerebrospinal fluid
... and cell culture and ...Inc; MBP: MoAb MCA70, AbD- Serotec©, Bio-Rad Laboratories, Inc; Neurofilament Heavy (NF-H): rabbit AHP2259GA, AbDSerotec© Bio-Rad La- boratories, Inc; Neurofilament Medium (NF-M): ...
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Antibody Responses to Acinetobacterspp. and Pseudomonas aeruginosa in Multiple Sclerosis: Prospects for Diagnosis Using the Myelin-Acinetobacter-Neurofilament Antibody Index
... ELISA. ELISAs were carried out as previously described (9). Briefly, aliquots of 200 l of bacterial suspension or bovine MBP or neurofilaments (25 g/ml; Sigma), diluted in a 0.05 M carbonate buffer (pH 9.6), ...
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Microglia exacerbate white matter injury via complement C3/C3aR pathway after hypoperfusion
... Iba-1-positive cells and blocking C3aR pathway suppressed detrimental microglial activation and alleviated white matter injury and cognitive decline after ...endothelial cells, the therapeutic effects of ...
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Strategies for the identification of loci responsible for the pathogenesis of multiple sclerosis
... 34. Smith, M. W., Patterson, N., Lautenberger, J. A., Truelove, A. L., McDonald, G. J., Waliszewska, A., Kessing, B. D., Malasky, M. J., Scafe, C., Le, E., De Jager, P. L., Mignault, A. A., Yi, Z., De The, G., Essex, M., ...
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