MCF-10A cells
Comparison of cytotoxicity and genotoxicity of 4 hydroxytamoxifen in combination with Tualang honey in MCF 7 and MCF 10A cells
... non-cancerous MCF- 10A cells and the effect increased with exposure time causing ...the MCF-7 cells, TH significantly amelio- rated the cytotoxic effect of the antioestrogen in ...
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Increasingly transformed MCF-10A cells have a progressively tumor-like phenotype in three-dimensional basement membrane culture
... MCF-10A cells were manipulated by the forced expression of a mutated H-ras-1, which is the human analog of the Harvey sarcoma virus ...resulting MCF- 10AT cells persisted as xenografts ...
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Arsenic, cadmium and neuron specific enolase (ENO2, γ-enolase) expression in breast cancer
... of MCF-10A cells with Cd +2 , the cells were injected under the renal capsule [24], a technique not available at this laboratory ’ s animal ...parent MCF-10A cells with ...
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Type I insulin like growth factor receptor over expression induces proliferation and anti apoptotic signaling in a three dimensional culture model of breast epithelial cells
... IGFIR-10A cells display notable similarities and differences with each of these ...these cells. Third, IGFIR structures retain some features of epithelial cells, such as secretion of certain ...
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Membrane associated collagen XIII promotes cancer metastasis and enhances anoikis resistance
... MDA-MB-231 cells (Fig. 3b). In contrast, MCF-10A cells with collagen XIII overexpression were more invasive than control cells ...in MCF-10A ...231 cells promotes ...
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Traditional Chinese medicinal formula Si-Wu-Tang prevents oxidative damage by activating Nrf2-mediated detoxifying/antioxidant genes
... in MCF-7 cells [30]. Consistent results have been seen in MCF-10A cells (data not ...in MCF-7 cells, the results obtained from MCF-7 cells showed higher fold ...
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An Active Form of Vav1 Induces Migration of Mammary Epithelial Cells by Stimulating Secretion of an Epidermal Growth Factor Receptor Ligand
... of MCF-10A cells was described in [70]. MCF-10A cells expressing the ecotropic receptor were made by retro- viral transfection of low passage MCF-10A cells ...
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Utilization of adipocyte-derived lipids and enhanced intracellular trafficking of fatty acids contribute to breast cancer progression
... epithelium MCF-10A cells (provided by the Key Labora- tory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences and Yunnan Province, Kunming Institute of Zoology, Kunming) ...
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The combinatorial activation of the PI3K and Ras/MAPK pathways is sufficient for aggressive tumor formation, while individual pathway activation supports cell persistence
... PTEN-/-KRAS(G12V) cells in ...that cells bearing the combination of PTEN loss and KRAS mutations also acquire a resistance to apoptosis, which could explain the dramatic increase in in vivo growth with the ...
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Loss of the N-terminal methyltransferase NRMT1 increases sensitivity to DNA damage and promotes mammary oncogenesis
... cancer cells more susceptible to DNA-damaging chemotherapies, many are also tumor suppressors because their loss results in an increase in genomic instability and oncogenic ...epithelial cells and a panel ...
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Dissecting the transcriptional networks underlying breast cancer: NR4A1 reduces the migration of normal and breast cancer cell lines
... MCF-10A cells (5 × 10 4 cells) were plated in the top chambers of 8 μm pore transwells (BD Biosciences) in the full culture medium minus EGF and were allowed to migrate towards medium ...
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Long non-coding RNA SNHG22 facilitates the malignant phenotypes in triple-negative breast cancer via sponging miR-324-3p and upregulating SUDS3
... (Fig. 2a). Further, RIP assay demonstrated that SNHG22 was largely concentrated in Ago2 group rather than in IgG group, indicating the existence of SNHG22 in Ago2- comprised RNA-induced silencing complexes (RISCs) (Fig. ...
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Amphiphilic Gemini Pyridinium mediated incorporation of Zn(II)meso tetrakis(4 carboxyphenyl)porphyrin into water soluble gold nanoparticles for photodynamic therapy
... In MCF-10A cells, the majority of nanoparticles were distributed around the nuclei forming aggregates of variable ...SKBR-3 cells were able to internalize 1·GNP but in a lesser quantity, and ...
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Morphine promotes cancer stem cell properties, contributing to chemoresistance in breast cancer
... treated MCF-7, BT549 and MCF-10A cells with morphine (0, 1, 10 μM), and performed a sphere formation assay [24, ...both MCF-7 cells (Figure 1A) and BT549 cells (Figure ...
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Mutations in the EGFR kinase domain mediate STAT3 activation via IL 6 production in human lung adenocarcinomas
... H3255 cells (Figure ...11-18 cells and applied to MCF-10A cells (which do not express pSTAT3) in the absence or presence of various blocking antibodies against gp130, IL-6, ...
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A novel MCF-10A line allowing conditional oncogene expression in 3D culture
... of MCF-10A cells in 3D culture (Figure ...of MCF- 10A cells and therefore, if expressed a priori, prevents acinar morphogenesis ...Therefore, MCF-10Atet cells ...
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miR 638 mediated regulation of BRCA1affects DNA repair and sensitivity to UV and cisplatin in triple negative breast cancer
... TNBC cells to DNA-damaging agents by means of their ability to reduce BRCA1 expres- sion and curb the activation of BRCA1 in TNBC ...cancer cells transfected with miR-638 mimic or inhibitor (Figure 4A) with ...
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Suberoylanilide Hydroxamic Acid as a Potential Therapeutic Agent for Human Breast Cancer Treatment
... cancer cells was not due to mutations in the gelsolin genes, but rather to the epigenetic changes in chromatin structure ...cancer cells, compared with normal, mortal human mammary epithe- lial ...
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Sclerotium rolfsii Lectin Induces Stronger Inhibition of Proliferation in Human Breast Cancer Cells than Normal Human Mammary Epithelial Cells by Induction of Cell Apoptosis
... of MCF-7 cells after treatment with SRL. MCF-7 cells were grown on cover slips and incubated with 20 mg/ml SRL for 36 h followed by staining with FITC-SRL, Anti-active caspase-3 primary ...
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Reversal by RARα agonist Am580 of c Myc induced imbalance in RARα/RARγ expression during MMTV Myc tumorigenesis
... Primary cells from MMTV-Myc tumors were isolated and ...pSG5-transfected cells to serve as an internal ...shRARg-transfected cells compared to the vector control cells (C and ...
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