MCF7 cells
Increased invasion and tumorigenicity capacity of CD44+/CD24- breast cancer MCF7 cells in vitro and in nude mice
... The CD44 antigen, encoded by the CD44 gene on chromosome 11, is a cell-surface glycoprotein involved in cell–cell interactions, cell adhesion, and migration [25]. CD44 has been reported as a cell surface marker for some ...
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Chloroformic fraction of Tanacetum vulgare L. induces cell cycle arrest and apoptosis in MCF7 cells
... Antitumor properties of chloroformic and aqueous methanolic fractions from crude aqueous ethanolic extract of Tanacetum vulgare L. aerial parts were examined against human cervix adenocarcinoma (HeLa), ovarian carcinoma ...
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RSK3/4 mediate resistance to PI3K pathway inhibitors in breast cancer
... RSK4 cells upon treatment with AZD6244 or MEK162, verifying that MEK inhibition downregulates the function of overexpressed RSK (Supplemental Figure ...renders cells resistant to the proapoptotic effects of ...
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Breast cancer specific mutations in CK1ε inhibit Wnt/β catenin and activate the Wnt/Rac1/JNK and NFAT pathways to decrease cell adhesion and promote cell migration
... of MCF7 epithelial breast cancer cells. The MCF7 cell line retains several characteristics of differentiated mammary epithelium, including the ability to process estradiol via cytoplasmic estrogen ...
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Synthesis of Anthracene Functionalised Fluorescent Amino Acids and Its Applications of Biosensing and Antibacterial Activity
... of MCF7 cell lines. MCF7 cells were stained with blue fluorescence and the anthracene amino acids hold promise as fluorescent imaging tools for biological ...the cells, we still need to ...
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17beta hydroxysteroid dehydrogenase type 1 modulates breast cancer protein profile and impacts cell migration
... Using the UniProt database [19], we determined the subcellular locations and functions (or biological pro- cesses) of each of the 59 proteins identified by MS analysis. The original spot for each protein, the spot ...
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Lysosomal Enzyme Induced Cell Death in MCF7 and Mammary Gland Cells
... in MCF7 and rat mammary gland cells, a histochemical acid phosphatase assay (Sigma, ...[17]. MCF7 cells (5 × 10 6 cells/mL) were grown on glass cover slides (Fisher Scientific, ...
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Mitochondrial mass, a new metabolic biomarker for stem-like cancer cells: Understanding WNT/FGF-driven anabolic signaling
... parental MCF7 cells, using FACS analysis and cell ...negative cells (little or no positive staining; mito-negative group); ii) bottom 5% (mito-low group); and top 5% (mito-high ...live cells ...
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High expression of MnSOD promotes survival of circulating breast cancer cells and increases their resistance to doxorubicin
... metastatic cells from SS-induced mitochondrial damage and ...staining MCF7 and MDA-MB-231 cells with MitoTracker Red-CMXRos after ...circulation. MCF7 cells were pre-treated with MnTBAP ...
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Estrogen receptor beta sensitizes breast cancer cells to the anti estrogenic actions of endoxifen
... of MCF7-ERb cell lines In order to determine the effects of ERb expression on the actions of endoxifen in breast cancer, we first devel- oped MCF7 cell lines stably expressing this ...receptor. MCF7 ...
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Nrf2 is the key to chemotherapy resistance in MCF7 breast cancer cells under hypoxia
... Hypoxia leads to reactive oxygen species (ROS) imbalance, which is proposed to associate with drug resistance and oncogenesis. Inhibition of enzymes of antioxidant balancing system in tumor cells was shown to ...
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Comparative cytotoxicity of artemisinin and cisplatin and their interactions with chlorogenic acids in MCF7 breast cancer cells
... In parts of Africa and Asia, self-medication with a hot water in- fusion of Artemisia annua (Artemisia tea) is a common practice for a number of ailments including malaria and cancer. In our earlier work, such an extract ...
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Loss of plakoglobin promotes decreased cell cell contact, increased invasion, and breast cancer cell dissemination in vivo
... and MCF7 2A-1 cells; however, NM23-H1 was unaltered in MCF7 3C-3 cells, which were significantly more migratory and inva- sive than MCF-7 2A-1 ...of cells from the primary tumor, ...
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BP1 transcriptionally activates bcl 2and inhibits TNFα induced cell death in MCF7 breast cancer cells
... cancer cells are associated with increased cell ...of MCF7 cells stably overexpressing BP1 were determined using MTT and Annexin V assays, and through examination of caspase ...in MCF7 breast ...
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Dichloroacetate potentiates tamoxifen-induced cell death in breast cancer cells via downregulation of the epidermal growth factor receptor
... the cells treated with siRNA against each isoform of ...the cells to ...co-treated cells was assessed using Annexin V/PI ...cancer cells was caused by the loss of mitochondrial membrane ...
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DNA replication stress mediates APOBEC3 family mutagenesis in breast cancer
... MCF10A cells, with a low basal level of replication stress, were treated with hydroxyurea in the presence of inhibitors of these sig- nalling pathways for 24 ...BT474 cells with U0126, rapamycin and ...
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Trefoil factor 3 promotes metastatic seeding and predicts poor survival outcome of patients with mammary carcinoma
... in MCF7 and T47D cells was modulated by forced expression or siRNA-mediated depletion of ...MC cells was determined in vitro and in ...MC cells stimulated colony scattering, cell adhesion to a ...
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Expression of Six1 in luminal breast cancers predicts poor prognosis and promotes increases in tumor initiating cells by activation of extracellular signal regulated kinase and transforming growth factor beta signaling pathways
... stem cells/tumor initiating cells ...in MCF7- Ctrl and MCF7-Six1 ...the MCF7- Six1 cells is dependent on TGF- b signaling by treating the cells with SB431542, which is ...
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PKCä Mediates Platelet Induced Breast Cancer Cell Invasion
... of MCF7-  ...in MCF7 cells (Liu et ...tumor cells (Kiley et ...tumor cells (McCracken et ...Caco-2 cells, suggesting that MMP-9 regulation by PKC ␦ could be observed in cancer ...
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Breast cancer stromal fibroblasts promote the generation of CD44+CD24- cells through SDF-1/CXCR4 interaction
... ments, cells were maintained at 37°C in a humidified 5% CO 2 /95% air ...When MCF7 cells were grown in suspension for six days, "primary mammospheres" were obtained, then collected by gravity ...
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