Melting-curve analysis
Identification and species-typing of wood rotting fungi using melting curve analysis
... The results showed that the combination of T(m)s of two types of fragment was sufficient to identify species and that the melting curve analysis is a simple but effective, low cost tool for ...
10
Detection and Differentiation of In Vitro Spiked Bacteria by Real Time PCR and Melting Curve Analysis
... We introduce a consensus real-time PCR protocol for the detection of bacterial DNA from laboratory- prepared specimens such as water, urine, and plasma. This prototype detection system enables an exact Gram stain ...
6
McSpoligotyping, a One Step Melting Curve Analysis Based Protocol for Spoligotyping of Mycobacterium tuberculosis
... DISCUSSION In this study, we described for the first time a single-step spoligotyping protocol based on melting curve analysis. As no post-PCR manipulations were required, the protocol was simple and ...
8
Detection and Genotyping of Oocysts of Cryptosporidium parvum by Real Time PCR and Melting Curve Analysis
... I melting curve analysis was used to confirm the specificity of the method when DNA extracted from fecal samples spiked with oocysts was ...of melting curve ...of melting ...
8
High Resolution Melting Curve Analysis for Rapid Detection of Rifampin Resistance in Mycobacterium tuberculosis: a Meta Analysis
... High-resolution melting curve analysis has been used for the detection of rifampin resistance in Mycobacterium tuberculosis and has shown ...meta- analysis of published studies to evaluate the ...
6
Detection of methylation in promoter sequences by melting curve analysis-based semiquantitative real time PCR
... in melting tem- perature (Tm) between methylated and unmethylated DNA after bisulfite ...a melting curve of the PCR product is ...the melting temperature of the DNA ...During melting ...
14
High Resolution Melting Curve Analysis for Rapid Detection of Pyrazinamide Resistance in Mycobacterium tuberculosis Clinical Isolates
... Resolution Melting Curve Analysis, Drug Resistance, pnc A Gene How to cite this paper: Osman, ...Resolution Melting Curve Analysis for Rapid Detection of Pyrazina- mide ...
18
Simultaneous Detection, Genotyping, and Quantification of Human Papillomaviruses by Multicolor Real Time PCR and Melting Curve Analysis
... and melting curve analysis under asymmetric PCR conditions ...while melting curve analysis reveals the sequence infor- mation, use of the combination of them would allow the ...
7
Detection of Anti Hepatitis B Virus Drug Resistance Mutations Based on Multicolor Melting Curve Analysis
... Nevertheless, it is far from practical due to the limited coverage of mutation types (16). We previously reported a multiplex real-time PCR detection strategy based on multicolor melting curve ...
8
Analysis of multiple single nucleotide polymorphisms closely positioned in the ovine PRNPgene using linear fluorescent probes and melting curve analysis
... imbalanced melting peaks were reliably detected. The bottle-neck for analysis within our laboratory was the number of PCR plates that could be prepared per ...day. Melting curve ...
10
Multiplex Real Time PCR Assay and Melting Curve Analysis for Identifying Mycobacterium tuberculosis Complex and Nontuberculous Mycobacteria
... I n South Korea, tuberculosis remains a serious public health problem, although its incidence rate has been greatly reduced (33). Recently, the isolation of nontuberculous mycobacteria (NTM) from clinical specimens has ...
5
Rapid Detection of Isoniazid Resistance in Mycobacterium tuberculosis Isolates by Use of Real Time PCR Based Melting Curve Analysis
... In conclusion, despite the fact that various MDR assays have been developed and evaluated over a period of years, the MeltPro TB/INH assay is among the first commercially available tests ded- icated to detection of INH ...
9
Multiplex ligation reaction based on probe melting curve analysis: a pragmatic approach for the identification of 30 common Salmonella serovars
... Discussion Salmonella infection remains a significant public health challenge worldwide, and given the paramount importance of serotyping as a crucial tool for epidemiological investiga- tions and surveillance of ...
10
Detection of monoclonal immunoglobulin heavy chain gene rearrangement (FR3) in Thai malignant lymphoma by High Resolution Melting curve analysis
... Resolution Melting curve analysis, which is faster, simpler and less expensive, was developed as the latest method for product analysis in molecular diagnos- tics ...lution Melting ...
9
Rapid detection of immunoglobulin heavy chain gene rearrangement by PCR and melting curve analysis using combined FR2 and FR3 primers
... by melting curve analysis in LightCycler system, which is based on a fundamental DNA ...DNA melting curve analysis in a closed system reduces cross-contamination ...
8
Simultaneous Quantification and Genotyping of Hepatitis B Virus for Genotypes A to G by Real Time PCR and Two Step Melting Curve Analysis
... Principle of real-time PCR genotyping by melting curve analysis. The geno- typing method was based on MCA with LightCycler hybridization probes as shown in Fig. 1A. The primers and hybridization ...
7
A novel protocol to isolate, detect and differentiate taeniid eggs in leafy greens and berries using real time PCR with melting curve analysis
... multilocularis, E. granulosus (s.l.) and Taenia spp. Methods: We compared the efficacy of different wash solutions to remove Taenia spp. eggs from spiked produce, assessed two DNA extraction kits for their performance on ...
12
Detection of a Knockdown Resistance Mutation Associated with Permethrin Resistance in the Body Louse Pediculus humanus corporis by Use of Melting Curve Analysis Genotyping
... Melting curve analysis genotyping. The real-time PCR tech- nique was first improved by testing the known genotype clones. Each of the three mutations was targeted individually. The results obtained ...
5
Comparison of Pyrosequencing, Sanger Sequencing, and Melting Curve Analysis for Detection of Low Frequency Macrolide Resistant Mycoplasma pneumoniae Quasispecies in Respiratory Specimens
... the melting curve analysis, the WT genotype could be readily differentiated from the mutant ...high-resolution melting curve analysis to de- termine mutations associated with ...
7
Molecular xenomonitoring of Dirofilaria immitis and Dirofilaria repens in mosquitoes from north eastern Italy by real time PCR coupled with melting curve analysis
... 29. Lee SE, Kim HC, Chong ST, Klein TA, Lee WJ: Molecular survey of Dirofilaria immitis and Dirofilaria repens by direct PCR for wild caught mosquitoes in the Republic of Korea. Vet Parasitol 2007, 148:149 – 155. 30. ...
8