MEMBRANE MICRODOMAINS
Multimerization of Human Immunodeficiency Virus Type 1 Gag Promotes Its Localization to Barges, Raft-Like Membrane Microdomains
... plasma membrane of infected ...raft-like membrane microdomains that we have termed ...barge membrane microdomains plays an important role in the HIV-1 assembly ...
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Heterogeneity of Raft-Type Membrane Microdomains Associated with VP4, the Rotavirus Spike Protein, in Caco-2 and MA 104 Cells
... Previous studies have shown that rotavirus virions, a major cause of infantile diarrhea, assemble within small intestinal enterocytes and are released at the apical pole without significant cell lysis. In contrast, for ...
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Alteration of actin dependent signaling pathways associated with membrane microdomains in hyperlipidemia
... Detergent resistant membrane microdomains isolation DRM microdomains fractions were prepared as previ- ously described [18]. Briefly, 200 mg of lung tissue fragments were solubilized on ice in 1.8 ml ...
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Organization of the Vesicular Stomatitis Virus Glycoprotein into Membrane Microdomains Occurs Independently of Intracellular Viral Components
... into membrane microdomains with diameters of 100 to 150 nm, with smaller amounts organized into microdomains of larger ...plasma membrane that are outside of virus budding sites as well as in ...
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Assembly of Arenavirus Envelope Glycoprotein GPC in Detergent-Soluble Membrane Microdomains
... membrane microdomains. Because the size of typical mem- brane microdomains is at or below the limit of resolution of confocal microscopy ( ⬍ 200 nm), we further analyzed the dis- tribution of GPC on ...
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Human Immunodeficiency Virus Type 1 and Influenza Virus Exit via Different Membrane Microdomains
... plasma membrane, as made evident in this report ...plasma membrane microdomains seemingly is in conflict with recent reports showing that HA can be incorporated into HIV-1- or SIV-based vectors (6, ...
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West Nile Virus Entry Requires Cholesterol-Rich Membrane Microdomains and Is Independent of αvβ3 Integrin
... West Nile virus (WNV) has been the leading cause of viral encephalitis in the United States since 1999. The endocytic processes involved in the internalization of infectious WNV by various cell types are not well ...
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Human Immunodeficiency Virus Type 1 Nef protein modulates the lipid composition of virions and host cell membrane microdomains
... additional membrane targeting motifs, a subpopulation of Nef resides in detergent resist- ant membrane microdomains (DRMs) or lipid rafts [18- ...these membrane microdomains in living ...
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Pseudotypes of Vesicular Stomatitis Virus with CD4 Formed by Clustering of Membrane Microdomains during Budding
... CD4-containing microdomains are similar in ...brane microdomains in BHK cells or whether it was distributed randomly in the plasma ...into membrane microdomains ...
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Plasma Membrane Microdomains Containing Vesicular Stomatitis Virus M Protein Are Separate from Microdomains Containing G Protein and Nucleocapsids
... 100-nm membrane microdomains (Fig. 2) and that M protein microdomains are formed in the absence of other VSV components ...protein microdomains is consistent with previous data on the interac- ...
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Coronavirus and Influenza Virus Proteolytic Priming Takes Place in Tetraspanin-Enriched Membrane Microdomains
... TEMs contain coronavirus receptors and priming proteases. Our findings fit with the hypothesis that infecting viruses encoun- ter cell receptors and priming proteases at TEM locations. Anti- body binding to TEMs, or ...
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Kinetic analysis of antagonist occupied adenosine A3 receptors within membrane microdomains of individual cells provides evidence of receptor dimerization and allosterism
... membrane compartments and microdomains is becom- ing increasingly evident (11–14). This spatial segrega- tion provides a mechanism by which intracellular sig- naling can be orchestrated within a cell in a ...
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Diet-induced hypercholesterolemia promotes androgen- independent prostate cancer metastasis via IQGAP1 and caveolin-1
... cholesterol membrane microdomains (Figure ...rich membrane domains. While IQGAP1 membrane targeting is essential for its regulation of migration, to our knowledge, this is the first report of ...
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Insulin Signaling in Microdomains of the Plasma Membrane
... A potential clue towards identifying the PI 3-kinase- independent arm of insulin action emerged from the idea that signal initiation might be segregated into discrete compartments in the plasma membrane. One ...
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Virus Particle Release from Glycosphingolipid-Enriched Microdomains Is Essential for Dendritic Cell-Mediated Capture and Transfer of HIV-1 and Henipavirus
... plasma membrane, we hypothesized that the virus assembly and budding site determines the ability of HIV-1 to interact with ...the membrane-targeting domain of the HIV-1 matrix (MA) protein that altered the ...
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Gag Induces the Coalescence of Clustered Lipid Rafts and Tetraspanin-Enriched Microdomains at HIV-1 Assembly Sites on the Plasma Membrane
... general membrane phenomenon underlies this coalescence of microdomains—a property or function of the membrane that Gag derivatives can affect regardless of membrane binding ...for ...
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p27Kip1 Localizes to Detergent-insoluble Microdomains Within Lymphocyte Membranes
... the membrane, rather than associ- ated in a random ...to membrane microdomains enriched in cholesterol and gly- cosphingoglycolipids ...the membrane in lymphocytes is through transport from ...
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The cytosolic N-terminus of CD317/tetherin is a membrane microdomain exclusion motif.
... to membrane microdomains with similar efficiency to the DGPI- CD40 protein (Andrew et ...contrasting membrane microdomain affinities of the CD317 DGPI chimeric constructs we have generated and those ...
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Segregation of CD4 and CXCR4 into Distinct Lipid Microdomains in T Lymphocytes Suggests a Mechanism for Membrane Destabilization by Human Immunodeficiency Virus
... plasma membrane that is disrupted by extraction of cholesterol with filipin or methyl--cyclodextrin but is resistant to extraction with 1% Triton X-100 at 0°C ...
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Dissecting Rotavirus Particle-Raft Interaction with Small Interfering RNAs: Insights into Rotavirus Transit through the Secretory Pathway
... rotavirus membrane enveloped viral intermediates from in- fected MA104 cells showed that these contain VP4, VP7, and NSP4 and that even after detergent extraction, VP4 remained attached to the ...
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