Membrane Permeabilization
Pneumolysin Activates Macrophage Lysosomal Membrane Permeabilization and Executes Apoptosis by Distinct Mechanisms without Membrane Pore Formation
... lysosomal/phagolysosomal membrane permeabilization (LMP), an upstream event programing susceptibility to apoptosis, and to apoptosis execution via a mitochondrial pathway, through dis- tinct ...
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Ionic Strength- and Temperature-Induced KCa Shifts in the Uncoating Reaction of Rotavirus Strains RF and SA11: Correlation with Membrane Permeabilization
... cell membrane permeabilization was found, as shown by the release of carboxyfluorescein (CF) from CF-loaded intestinal brush-border membrane ...that membrane permeabilization must be ...
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Selective Membrane Permeabilization by the Rotavirus VP5* Protein Is Abrogated by Mutations in an Internal Hydrophobic Domain
... coli-based membrane permeabi- lization assays have also been used to monitor the function of proteins which fuse or permeabilize membranes and are well suited for analyzing mutagenized proteins ...potential ...
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Marine alkaloid Monanchocidin a overcomes drug resistance by induction of autophagy and lysosomal membrane permeabilization
... Monanchocidin A (MonA) is a novel alkaloid recently isolated from the marine sponge Monanchora pulchra. The compound reveals cytotoxic activity in genitourinary cancers including cisplatin-sensitive and -resistant germ ...
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Pheochromocytoma (PC 12) as a Model Cell Line for Membrane Permeabilization Studies in the presence of Electromagnetic Fields (EMFs): Recent Advances
... The pheochromocytoma PC 12 cell line is an established model system for neurosecretion and neuronal differentiation, particular to study cellular responses to nerve growth factors (NGF) and how these lead to expression ...
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Bilirubin and Amyloid-β Peptide Induce Cytochrome c Release Through Mitochondrial Membrane Permeabilization
... plasma membrane vesi- cles isolated from rat brain with bilirubin was shown to depress the membrane potential by a mechanism involving alterations in membrane permeability ...with membrane ...
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Pneumolysin Activates Macrophage Lysosomal Membrane Permeabilization and Executes Apoptosis by Distinct Mechanisms without Membrane Pore Formation
... To assess if LLA was strain or species specific, we assessed LLA in cells challenged with clinical strains of serotype 1 S. pneumoniae expressing hemolytic (strain ST227) (37) or nonhemolytic (strain ST306) PLY (38) or ...
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Cellular entry of nanoparticles via serum sensitive clathrin-mediated endocytosis, and plasma membrane permeabilization
... Next, several potential mechanisms for nanoparticle internalization into cells were evaluated. In both serum-free and serum-containing studies, it was demonstrated that these nanoparticles can enter cells through ...
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Lysosomal Membrane Permeabilization is an Early Event in Sigma-2 Receptor Ligand Mediated Cell Death in Pancreatic Cancer
... cell membrane components in Bxpc3 and Aspc1 pancreatic cancer cells and accumulate in ...lysosomal membrane glycoprotein, LAMP1, with shRNA greatly reduced the viability of cells following ...lysosomal ...
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The oncolytic peptide LTX-315 kills cancer cells through Bax/ Bak-regulated mitochondrial membrane permeabilization
... the permeabilization of the outer mitochondrial membrane causing the release of SMAC and cytochrome ...plasma membrane permeabilization studied in erythrocytes (which lack ...
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Membrane Permeabilization by Small Hydrophobic Nonstructural Proteins of Japanese Encephalitis Virus
... ER membrane at least once (reviewed in reference ...the membrane, viroporins usually contain a short stretch of basic amino acids flanked by a membrane- interacting domain, a unique feature seen in ...
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Rotaviruses induce an early membrane permeabilization of MA104 cells and do not require a low intracellular Ca2+ concentration to initiate their replication cycle.
... The permeabilization of the membrane was transient and was not detected 15 min after virus entry (the length of incu- bation with neutralizing MAb 159), suggesting that the poten- tial pore or channel that ...
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Modulating lysosomal function through lysosome membrane permeabilization or autophagy suppression restores sensitivity to cisplatin in refractory non small cell lung cancer cells
... In an attempt to clarify the caspases involved in the drugs combination-induced cell death, we measured the activity of caspase 3, 8 and 9 in treated A549cisR and A549Pt cells. While chloroquine alone did not elicit ...
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Coupling of lysosomal and mitochondrial membrane permeabilization in trypanolysis by APOL1
... mitochondrial membrane permeabilization (LMP and ...mitochondrial membrane fenestration, which can be mimicked by knockdown of a mitochondrial mitofusin-like protein ...
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Commitment to Apoptosis in CD4+ T Lymphocytes Productively Infected with Human Immunodeficiency Virus Type 1 Is Initiated by Lysosomal Membrane Permeabilization, Itself Induced by the Isolated Expression of the Viral Protein Nef
... struct did we observe the expected downregulation of CD4 expression on the cell surface (Fig. 6A) (the results for the 24 h posttransfection time are presented). Nef has been reported to promote Fas ligand transcription ...
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Membrane permeabilization by different regions of the human immunodeficiency virus type 1 transmembrane glycoprotein gp41.
... (49). The presence of the T7 lysozyme also makes the cells more susceptible to lysis when the bacterial membrane is al- tered (34). Addition of rifampin together with IPTG blocks the expression of the E. coli ...
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Important factors for cell-membrane permeabilization by gold nanoparticles activated by nanosecond-laser irradiation
... irradiation, membrane permeability of OVCAR-3 cells was studied under different conditions of AuNP concentra- tion, cell-incubation medium, and cell–AuNP incubation ...time. Membrane permeability and cell ...
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High glucose–induced ROS activates TRPM2 to trigger lysosomal membrane permeabilization and Zn2+-mediated mitochondrial fission
... We showed that plasma membrane TRPM2 channels responded to oxidative stress to generate Ca2+ signals, which induced lysosomal membrane permeabilisation leading to the mobilisation of lys[r] ...
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Inhibitors of poliovirus uncoating efficiently block the early membrane permeabilization induced by virus particles.
... These results indicate that active viral replication is required to fully permeabilize cells late during infection and suggest that the blockade of alphasarcin entry by uncoating inhibit[r] ...
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A Proapoptotic Peptide Derived from Reovirus Outer Capsid Protein μ1 Has Membrane-Destabilizing Activity
... direct membrane-destabilizing activity and can target mitochondrial ...during membrane penetration or produced in the cytosol as a consequence of the action of cytosolic proteases can target and destabilize ...
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