merozoite surface antigen 1
Naturally acquired cellular immune response against Plasmodium vivax merozoite surface protein 1 paralog antigen
... The gene fragment encoding PvMSP1P-19 was amplified by PCR from genomic DNA of the P. vivax Sal I strain se- quence, and cloned into the Xho I and Not I sites of the pEU- E01-His-TEV-MCS vector (CellFree Sciences, ...
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Spherical Body Protein 4 Is a New Serological Antigen for Global Detection of Babesia bovis Infection in Cattle
... including merozoite surface antigen 2c (BbMSA-2c), C-terminal rhoptry-associated protein 1 (BbRAP-1/CT), truncated thrombospondin-related anonymous protein (BbTRAP-T), spherical body ...
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Antibody responses to, and the structure of Plasmodium falciparum merozoite surface protein-1: A candidate malaria vaccine antigen
... The results described in the previous chapter clearly showed a cause-effect association between antibody-mediated inhibition o f MSP-1 secondary processing, and inhibition of erythrocyte invasion by the malaria ...
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Prediction of Merozoite Surface Protein 1 and Apical Membrane Antigen 1 Vaccine Efficacies against Plasmodium chabaudi Malaria Based on Prechallenge Antibody Responses
... on antigen-coated wells at dilutions that ranged from 1:100 to ...1:3,200,000. Antigen-specific antibodies were detected with horseradish peroxi- dase-conjugated rabbit antibody specific for ...
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Immunogenetic markers associated with a naturally acquired humoral immune response against an N terminal antigen of Plasmodium vivax merozoite surface protein 1 (PvMSP 1)
... diluted 1/100 was added to the wells in duplicate and incubated for 1 h at 37 ...diluted 1/1000 were added to each well for the detection of total IgG or IgM, ...HP6050, 1/2500 for IgG2 clone ...
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Antibody response dynamics to the Plasmodium falciparum conserved vaccine candidate antigen, merozoite surface protein 1 C terminal 19kD (MSP1 19kD), in Peruvians exposed to hypoendemic malaria transmission
... ure 1). For example, in the 2003 malaria season commu- nity survey (March 2003), 34.8% of children and 76.8% of adults were IgG-positive (point seroprevalence). Despite low transmission, the αMSP1-19kD seropreva- ...
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Genetic polymorphism of merozoite surface protein 1 and merozoite surface protein 2 in Plasmodium falciparum field isolates from Myanmar
... population [36]. Multiple clonal infections with different genotypes of P. falciparum were identified among Myan- mar P. falciparum isolates in a high proportion (79.4% for MSP-1 and 87.3% for MSP-2). And a high ...
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Conformational Dependence and Conservation of an Immunodominant Epitope within the Babesia equi Erythrocyte-Stage Surface Protein Equi Merozoite Antigen 1
... Equi merozoite antigen 1 (EMA-1) is an immunodominant Babesia equi erythrocyte-stage surface protein. A competitive enzyme-linked immunosorbent assay (ELISA), based on inhibition of ...
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Expression of Babesia equi Merozoite Antigen 1 in Insect Cells by Recombinant Baculovirus and Evaluation of Its Diagnostic Potential in an Enzyme Linked Immunosorbent Assay
... cell surface, as seen in the B. equi parasite. Although the EMA-1 had a typical membrane protein structure, as described above, some EMA-1 was secreted into the supernatant of recombinant ...
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Molecular identification and antigenic characterization of a merozoite surface antigen and a secreted antigen of Babesia canis (BcMSA1 and BcSA1)
... electrophoresis (SDS-PAGE) and Western blot analysis The recombinant BcMSA1 and BcSA1 proteins were verified by 10 % SDS-PAGE. Furthermore, mouse anti- rBcMSA1 and anti-rBcSA1 sera were used to detect the native BcMSA1 ...
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Variation in the immune responses against Plasmodium falciparum merozoite surface protein 1 and apical membrane antigen 1 in children residing in the different epidemiological strata of malaria in Cameroon
... 32]. The high proportion of IgG responders in this study could be due to the constant exposure to infective bites of mosquitoes or the participants sustained an immune response after active infection [3]. MSP-1 ...
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Identification of a novel merozoite surface antigen of Plasmodium vivax, PvMSA180
... Plasmodium vivax parasites were collected from patients with malaria in Thailand and spotted onto eight-well glass slides. The slides were fixed in ice-cold acetone for 10 min, dried, and blocked in 5% BSA in PBS-T at 37 ...
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Interferon gamma responses to Plasmodium falciparum liver stage antigen 1 and merozoite surface protein 1 increase with age in children in a malaria holoendemic area of western Kenya
... In previous studies in a highland area with highly sea- sonal malaria, it has been documented that IL-10 responses to LSA-1 were similar in children and adults and decreased dramatically in the absence of high ...
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Results from tandem Phase 1 studies evaluating the safety, reactogenicity and immunogenicity of the vaccine candidate antigen Plasmodium falciparum FVO merozoite surface protein 1 (MSP142) administered intramuscularly with adjuvant system AS01
... of antigen quality and protective potential, there is little direct evidence support- ing this concept outside of findings from epidemiologic studies evaluating the relationship of MSP1 antibody titres and ...
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Comparative analysis of the profiles of IgG subclass specific responses to Plasmodium falciparum apical membrane antigen 1 and merozoite surface protein 1 in naturally exposed individuals living in malaria hypoendemic settings, Iran
... mMTris-HCl, 1 M NaCl, and 60 mM imidazole, pH ...(with 1% SDS and 2% β-mercaptoethanol [2ME]) and non-reducing conditions (with SDS and with- out 2ME) by sodium dodecyl sulfate-polyacrylamide gel ...
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IMMUNOGENICITY OF THE MEROZOITE SURFACE PROTEIN-1 (MSP-1) OF HUMAN PLASMODIUM SP.
... Development of malaria vaccines by using MSP-1 encounters numerous challenges. Knowledge about the real functions of this antigen and its interactive mechanisms with human host cells are limited. The ...
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Genetic diversity of the msp 1, msp 2, and glurp genes of Plasmodium falciparum isolates in Northwest Ethiopia
... Background: Determination of the genetic diversity of malaria parasites can inform the intensity of transmission and identify potential deficiencies in malaria control programmes. This study was conducted to characterize ...
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PCR/RFLP Based Analysis of Genetically Distinct Plasmodium vivax Population of Pvmsp 3α and Pvmsp 3β genes in Pakistan
... By using PCR technique, the allelic variants of P. vivax merozoite surface protein-3α were detected by standard protocol of Bruce et al. [11]. Primary PCR of Pvmsp-3α was performed in 20 ml using 3 ml of ...
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Genetic polymorphism of merozoite surface protein 1 and merozoite surface protein 2 in Plasmodium falciparum isolates from Brazzaville, Republic of Congo
... Allelic specific P. falciparum msp-1 and msp-2 geno- typing has shown that malaria parasite population in Brazzaville is highly diverse. However, the number of alleles may have been underestimated due to the limit ...
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In vitro invasion inhibition assay using antibodies against Plasmodium knowlesi Duffy binding protein alpha and apical membrane antigen protein 1 in human erythrocyte adapted P knowlesi A1 H 1 strain
... The DNA fragment of PkDBPα-II was amplified from the P. knowlesi A1-H.1 strain with specific primers PkDBPα_F (actgatccatggTTA TTA ATC AAA CTT TTC TTC); PkDBPα_R (atagtttagcggccgcTCA GAG ATG ATG ATG ATG ATG TTC ...
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