merozoite surface antigen 2
Comparative analysis of the profiles of IgG subclass specific responses to Plasmodium falciparum apical membrane antigen 1 and merozoite surface protein 1 in naturally exposed individuals living in malaria hypoendemic settings, Iran
... This study was carried out in Chabahar, Sistan and Baluchistan Province in south-eastern Iran. In this area, most of the patients are adults and may experience several infections by P. falciparum and P. vivax . In this ...
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Molecular identification and antigenic characterization of a merozoite surface antigen and a secreted antigen of Babesia canis (BcMSA1 and BcSA1)
... The B. canis-infected dog sera were used to immune- screen the cDNA expression library. The cDNA library was plated at a density of 1.5 × 10 4 plaque-forming units (PFUs) per 100 cm 2 square plate on a total of 20 ...
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Plasmodium falciparum merozoite surface antigen specific cytophilic IgG and control of malaria infection in a Beninese birth cohort
... Plasmodium falciparum malaria remains a significant cause of infant mortality and morbidity in many parts of the world especially in sub-Saharan Africa. In 2017, there were an estimated 219 million cases of malaria and ...
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Naturally acquired cellular immune response against Plasmodium vivax merozoite surface protein 1 paralog antigen
... Heparinized whole blood was diluted in incomplete RPMI 1640 medium at a dilution of 1:1. PBMCs were isolated by overlaying with Ficoll-Hypaque (Stemcell Technologies, Vancouver, CA, USA) and centrifuged at 2,000 rpm, ...
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Genetic polymorphism of merozoite surface protein 1 and merozoite surface protein 2 in Plasmodium falciparum field isolates from Myanmar
... population [36]. Multiple clonal infections with different genotypes of P. falciparum were identified among Myan- mar P. falciparum isolates in a high proportion (79.4% for MSP-1 and 87.3% for MSP-2). And a high ...
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IMMUNOGENICITY OF THE MEROZOITE SURFACE PROTEIN-1 (MSP-1) OF HUMAN PLASMODIUM SP.
... target antigen could be used in vaccine development, immunization study with animal models is a crucial step to validate the immunogenicity and immunoprotectivity of the target ...the merozoite invasion, ...
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Results from tandem Phase 1 studies evaluating the safety, reactogenicity and immunogenicity of the vaccine candidate antigen Plasmodium falciparum FVO merozoite surface protein 1 (MSP142) administered intramuscularly with adjuvant system AS01
... 10 μg group began in April 2008 with vaccinations for the 50 μg group starting three weeks later after safety review. One subject in the low-dose group was withdrawn after the first vaccination due to a prolonged ...
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Antibody responses to, and the structure of Plasmodium falciparum merozoite surface protein-1: A candidate malaria vaccine antigen
... in merozoite preparations was by a modification of an assay described previously (Blackman et ...and 2 mM MgCb, supplemented with protease inhibitors antipain, leupeptin, aprotinin and TLCK (reaction ...for ...
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Antibody response dynamics to the Plasmodium falciparum conserved vaccine candidate antigen, merozoite surface protein 1 C terminal 19kD (MSP1 19kD), in Peruvians exposed to hypoendemic malaria transmission
... Although our IgG subclass analysis has not yet been com- pleted, it is interesting to consider that if IgG subclass switching predicts longer duration antibody production, then the results of Tongren et al. suggest that ...
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Conformational Dependence and Conservation of an Immunodominant Epitope within the Babesia equi Erythrocyte-Stage Surface Protein Equi Merozoite Antigen 1
... Equi merozoite antigen 1 (EMA-1) is an immunodominant Babesia equi erythrocyte-stage surface protein. A competitive enzyme-linked immunosorbent assay (ELISA), based on inhibition of monoclonal ...
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Expression of Equi Merozoite Antigen 2 during Development of Babesia equi in the Midgut and Salivary Gland of the Vector Tick Boophilus microplus
... common surface proteins throughout multiple distinct stages of the par- asite or regulation of novel surface molecules by the distinct life cycle stages within the mammalian host and the ...
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Variation in the immune responses against Plasmodium falciparum merozoite surface protein 1 and apical membrane antigen 1 in children residing in the different epidemiological strata of malaria in Cameroon
... Malaria constitutes a major health problem in Cam- eroon where it accounts for 48% of all hospital admis- sion, 30% of morbidity and 67% of childhood mortality per year [1, 2]. Malaria in Cameroon is caused ...
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Identification of a novel merozoite surface antigen of Plasmodium vivax, PvMSA180
... Plasmodium vivax parasites were collected from patients with malaria in Thailand and spotted onto eight-well glass slides. The slides were fixed in ice-cold acetone for 10 min, dried, and blocked in 5% BSA in PBS-T at 37 ...
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Antibodies to Plasmodium falciparum merozoite surface protein 1p19 malaria vaccine candidate induce antibody dependent respiratory burst in human neutrophils
... its 2 EGF-domain structure, and can be brought about by an orthologous domain with differing ...specific antigen, are still ...of antigen–antibody com- plexes in solution and subsequent removal by ...
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Spherical Body Protein 4 Is a New Serological Antigen for Global Detection of Babesia bovis Infection in Cattle
... test in recent decades, it is time-consuming and subjective. Additionally, it cannot be automated, and results are largely influenced by the degree of training of the technician. Re- cently, the competitive ELISA ...
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Natural selection and genetic diversity of domain I of Plasmodium falciparum apical membrane antigen 1 on Bioko Island
... To assess the association between host immune pres- sure and natural selection of PfAMA-1, genetic polymor- phisms in predicted B-cell epitopes and IUR regions were analysed. Based on a recent report, detailed ...
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Diversity and evolutionary genetics of the three major Plasmodium vivax merozoite genes participating in reticulocyte invasion in southern Mexico
... Plasmodium vivax is the main species causing malaria in the Middle East, Asia, the West Pacific, and Central and South America, where billions of people are at risk [1, 2]. In the Americas, the number of malaria ...
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Genetic polymorphism of merozoite surface protein 2 and prevalence of K76T pfcrt mutation in Plasmodium falciparum field isolates from Congolese children with asymptomatic infections
... Alleles were determined by size and family type. Allele typing showed the highly polymorphic nature of P. fal- ciparum isolates. Both 3D7 and FC27 families were identified with 8 and 10 different alleles respectively ...
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Extensive diversity in the allelic frequency of Plasmodium falciparum merozoite surface proteins and glutamate rich protein in rural and urban settings of southwestern Nigeria
... This study evaluated the genetic diversity of P. falciparum polymorphic markers (msp-1, msp-2 and glurp genes) in children from urban and rural communities of Oyo State, southwest Nigeria. Understanding the ...
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Antibody levels against GLURP R2, MSP1 block 2 hybrid and AS202 11 and the risk of malaria in children living in hyperendemic (Burkina Faso) and hypo endemic (Ghana) areas
... risk of symptomatic malaria infection in Burkinabe [20] and Ghanaian [21] children. Alpha (α) helical coiled motifs in malaria antigens, such as MSP3 and MSP6, are important oligomerization sub-units and targets of ...
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