merozoite surface protein 2
Genetic polymorphism of merozoite surface protein 2 and prevalence of K76T pfcrt mutation in Plasmodium falciparum field isolates from Congolese children with asymptomatic infections
... Genotyping of merozoite surface protein-2 (msp2) is the commonly used method to study the genetic diversity of P. falciparum. This gene presents polymorphisms in the number of repeats and ...
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Genetic polymorphism of merozoite surface protein 1 and merozoite surface protein 2 in Plasmodium falciparum isolates from Brazzaville, Republic of Congo
... However, in contrast with the first report in Brazzaville [5], P. falciparum infection in this symptomatic population shows a significant correlation between parasite density and the number of parasite genotype. This ...
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Genetic polymorphism of merozoite surface protein 1 and merozoite surface protein 2 in Plasmodium falciparum field isolates from Myanmar
... leading to a change in the open reading frame [14]. The PNA motif at the 3' end of E1 was duplicated in allele 3. Several nucleotide substitutions, all leading to non-syn- onymous amino acid changes in E1, R1, R2, and E3 ...
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Changing pattern of the genetic diversities of Plasmodium falciparum merozoite surface protein 1 and merozoite surface protein 2 in Myanmar isolates
... block 2 region of all alleles belonging to K1 type consistently started with SAQ, and terminated with SGT or SGP (Additional file 2: Table ...and 2 tri-morphic amino acid changes were found at 15 ...
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American Journal of Microbiological Research
... for Merozoite Surface Protein 2 (MSP2) alone, 34 and 31 had distinct MSP2 alleles detected in the uncomplicated and asymptomatic malaria groups respectively in Lafia, Nassarawa State, in ...
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Genetic diversity among Plasmodium falciparum field isolates in Pakistan measured with PCR genotyping of the merozoite surface protein 1 and 2
... the surface antigens mero- zoite surface protein 1 (msp-1), merozoite surface protein 2 (msp-2) and the glutamate-rich protein (glurp) ...
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Extensive diversity in the allelic frequency of Plasmodium falciparum merozoite surface proteins and glutamate rich protein in rural and urban settings of southwestern Nigeria
... and 2 are erythrocyte membrane-bound proteins abundantly found on the blood stages of ...the protein particularly in msp- 1-block 2 regions which has three allelic groups ...regions. Merozoite ...
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Genetic diversity in the block 2 region of the merozoite surface protein 1 of Plasmodium falciparum in central India
... The 555 bp polymorphic region of block 2, merozoite surface protein 1 gene was amplified. A total of 169 P. falciparum infected blood samples from 2005 and 165 samples from 2009 were used for ...
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Polymorphism of the merozoite surface protein 1 block 2 region in Plasmodium falciparum isolates from Mauritania
... During the study, if patient presented to health facilities with symptoms consistent with mild malaria, including fever, chills, headache, and a positive slide, they were of- fered standard ACT as first-line treatment. ...
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Polymorphic patterns of the merozoite surface protein 3β in Korean isolates of Plasmodium vivax
... A total of 95 blood samples used in this study were col- lected from Korean patients infected with P. vivax in Korea between 2007 and 2012 (2007–2010, n = 15 for each year; 2011, n = 18; 2012, n = 17). All the patients ...
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Genetic diversity of Plasmodium vivax isolates from Azerbaijan
... Merozoite surface protein 1 (MSP-1) marker analyses A portion of the MSP-1 gene (the region encompassing the interspecies conserved blocks ICB5 and ICB6) was amplified using a nested PCR with, ...
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Multiplicity and molecular epidemiology of Plasmodium vivax and Plasmodium falciparum infections in East Africa
... Background: Parasite genetic diversity and multiplicity of infection (MOI) affect clinical outcomes, response to drug treatment and naturally‑acquired or vaccine‑induced immunity. Traditional methods often underestimate ...
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Immunogenicity of a chimeric Plasmodium falciparum merozoite surface protein vaccine in Aotus monkeys
... All monkeys were in good health, free of tuberculosis and weighed between 0.8 and 1.2 kg at the start of the study. Animals were weighed at weekly intervals. Blood and serum were collected at biweekly intervals begin- ...
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Genetic diversity of the merozoite surface protein 3 gene in Plasmodium falciparum populations in Thailand
... Fig. 2, the polymorphisms between the nucleo- tide positions 252–528, excluding monomorphic nucleo- tide positions 351–375, showed significant departure from neutrality in all three ...
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Identification and characterization of Pv50, a novel Plasmodium vivax merozoite surface protein
... The enriched schizont-stage parasites were purified by Percoll gradient centrifugation, spotted onto a multi-well slide, fixed in ice-cold acetone for 3 min, air-dried, and stored at -80 °C. Before use, the slides were ...
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Plasmodium falciparum merozoite surface protein 1: Antigenicity, immunogenicity and structure.
... takes 2 to 10 days depending on the Plasmodium species (5-6 days for ...of 2 days ...the merozoite surface and the erythrocyte ...The merozoite then reorients so that its apical end, ...
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Characterization of Pv92, a novel merozoite surface protein of Plasmodium vivax
... The amine-coated slides were prepared as described previ- ously [4]. A total of 32 or 20 serum samples from vivax pa- tients or healthy persons, respectively, were used to evaluate the antigenicity of Pv92. One-hundred ...
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Genetic analysis of the merozoite surface protein 1 block 2 allelic types in Plasmodium falciparum clinical isolates from Lao PDR
... 10 2 0 30 40 50 60 ....|. ...|... .|....| ....|.. ..|.... |....|. ...|... .|....| ....| Thai , T836 isolate AACTAG TACACCT GGTTCAG GTGGTTC AGTTACT TCAGGTG GTTCAGT TACTTCA GGTGG Laos , MAD20 type 1 (n =95)* ...... ...
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Merozoite surface protein-7. A novel protein on the surface of Plasmodium falciparum merozoites
... the merozoite surface, passing through pellicular membranes, to release their contents by exocytosis into the parasitophorous ...erythrocyte surface antigen (RESA) which either crosses the PVM to ...
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PCR/RFLP Based Analysis of Genetically Distinct Plasmodium vivax Population of Pvmsp 3α and Pvmsp 3β genes in Pakistan
... By using PCR technique, the allelic variants of P. vivax merozoite surface protein-3α were detected by standard protocol of Bruce et al. [11]. Primary PCR of Pvmsp-3α was performed in 20 ml using 3 ...
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