Metabolic Engineering in Plants
Engineering metabolic pathways in plants by multigene transformation
... of metabolic engineering in plants, a syn- thetic biology approach would utilize specific promoters, genes, and other regulatory elements to create ideal genetic circuits that facilitate the ...
12
Metabolic engineering of microorganisms for production of aromatic compounds
... in plants, stilbenes (stilbenoids) are polyphenolic secondary metabolites, which come to the fore with the 1,2-diphe- nylethylene ...defense plants against various environ- mental stresses such as wounding, ...
29
Improved production of fatty alcohols in cyanobacteria by metabolic engineering
... Fatty alcohols are important industrial chemicals. They can also be good biofuel additives because of their perfect fuel properties. In nature, fatty alcohols exist as free fatty alcohols or waxes (oxygen esters of ...
9
Engineering microbial chemical factories using metabolic models
... as plants) alike can be exploited to convert low value inputs into high value ...goal, metabolic and industrial engineers rely on different techniques for achieving productivity ...goals. Metabolic ...
11
Metabolic engineering of Saccharomyces cerevisiae for 7-dehydrocholesterol overproduction
... As proved in many cases, screening enzymes from diverse sources is a promising strategy to enhance the titer of desired product [22–24]. In this study, DHCR24 is the only heterologous protein which catalyzed the final ...
14
Metabolic engineering of Escherichia coli for the production of cinnamaldehyde
... Selection of enzymes for cinnamaldehyde biosynthesis Cinnamaldehyde can be synthesized from l-phenylala- nine and its biosynthesis requires three enzymatic reac- tions: (i) deamination of l-phenylalanine into cinnamic ...
12
Metabolic engineering of microbial cell factories for production of nutraceuticals
... in plants) that protect against stress conditions such as ultraviolet radiation, pathogenic infection and physical ...and metabolic syndromes [1, 2, 35, ...strain engineering efforts as describe ...
11
Metabolic engineering of Escherichia colifor the biosynthesis of alpha-pinene
... by tapping trees (gum turpentine) or as a byproduct of paper pulping (crude sulfate turpentine, CST) [2]. However, the extraction of these molecules from plants is tedious and inefficient and requires substantial ...
10
Metabolic engineering of Corynebacterium glutamicum for anthocyanin production
... this study, we found that codon optimization of ANS and 3GT had very limited positive effects on improving C3G production, indicating that the codon usage in C. glu- tamicum might fit well with that in plants for ...
13
26. Current approaches for enhancing secondary plant production in vitro
... medicinal plants. But metabolic engineering is needed to enhance the accumulation of medicinally important compounds from ...many plants including Arabidopsis and medicinal plants has ...
9
An ancient Chinese wisdom for metabolic engineering: Yin-Yang
... Second, metabolic engineers need to exploit native path- ways and avoid extensive pathway ...approach, metabolic engineers can rationally design their engineer- ing ...
9
Metabolic engineering of Saccharomyces cerevisiae for the production of n-butanol
... In an attempt to explain the differences in n-butanol pro- duction by the various strains, we analyzed the pathway intermediates. Previously, this analysis has proven to be successful in interrogating and optimizing ...
8
In silico characterization of microbial electrosynthesis for metabolic engineering of biochemicals
... wild-type metabolic network of ...A metabolic map describing the changes to fluxes in the central metabo- lism is provided in the Additional file ...
14
Metabolic engineering of Escherichia coli for enhanced arginine biosynthesis
... ArgO, encoded by the argO gene, is transcriptionally regu- lated by ArgP [13-15]. The latter is responsive to intracellu- lar arginine levels and activates the transcription of argO accordingly [15-17]. In addition, E. ...
11
Challenges and tackles in metabolic engineering for microbial production of carotenoids
... pathways. Engineering strategies include enhancement of IPP and DMAPP precursors supply by overexpression of rate- limiting enzyme of the MEP or MVA pathway, optimi- zation of heterologous crt pathway, and ...
8
A toolset of constitutive promoters for metabolic engineering of Rhodosporidium toruloides
... Promoters from R. toruloides that were posited to have medium or strong transcriptional activity in both directions were also investigated. Bidirectional promot- ers can reduce design and assembly complexity and also ...
11
Dynamic metabolic control : towards precision engineering of metabolism
... in metabolic engineering have led to the synthesis of a wide variety of valuable chemicals in ...natural metabolic control strategies and recent developments in how they inspire the ...
27
Metabolic engineering of Bacillus subtilis for growth on overflow metabolites
... glucose or anaerobic nitrate respiration [2,3] and lead to the formation of acetate as well as acetoin and 2,3- butanediol. Industrial fermentation processes are usually so designed that overflow metabolism is avoided or ...
10
Chromosome engineering of Escherichia coli for constitutive production of salvianic acid A
... Fig. 1 Chromosomal engineering for constitutive production of salvianic acid A (SAA) in E. coli. a The artificial synthetic pathway of SAA from glu‑ cose. b The targeted integration of three modules into ...
11
Modular systems metabolic engineering enables balancing of relevant pathways for l-histidine production with Corynebacterium glutamicum
... theoretical maximum, are crucial factors for industrial- scale application and therefore have to be optimized in further studies. So far, few studies provided knowledge for the targeted optimization of C. glutamicum as a ...
21