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Metaphase ii

Delaying the oocyte maturation trigger by one day leads to a higher metaphase II oocyte yield in IVF/ICSI: a randomised controlled trial

Delaying the oocyte maturation trigger by one day leads to a higher metaphase II oocyte yield in IVF/ICSI: a randomised controlled trial

... The results of the trial were processed anonymously by a single observer (FV). The number of metaphase II oocytes (MII) was our primary outcome variable. Sec- ondary variables that demonstrate the oocyte ...

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The 2 cell block occurring during development of outbred mouse embryos is rescued by cytoplasmic factors present in inbred metaphase II oocytes

The 2 cell block occurring during development of outbred mouse embryos is rescued by cytoplasmic factors present in inbred metaphase II oocytes

... the metaphase II (MII) oocyte is genetically programmed to express, during the very early stages of development, some factor(s) required to determine developmental competence beyond the 2- cell ...

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No effect of exogenous melatonin on development of cryopreserved metaphase II oocytes in mouse

No effect of exogenous melatonin on development of cryopreserved metaphase II oocytes in mouse

... Melatonin (N-acetyl-5-methoxytryptamine), a deriva- tive of tryptophan mainly produced in the pineal gland of vertebrates [20, 21], is a potent free radical scavenger and antioxidant [22, 23]. Melatonin and its ...

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Calmodulin dependent protein kinase gamma 3 (CamKIIγ3) mediates the cell cycle resumption of metaphase II eggs in mouse

Calmodulin dependent protein kinase gamma 3 (CamKIIγ3) mediates the cell cycle resumption of metaphase II eggs in mouse

... Fig. 3. CamKII MO inhibits exit from metaphase II arrest. (A)  Percentage meiotic resumption from metII arrest, as measured by second polar body (PB2) extrusion and pronucleus (PN) formation in in vitro ...

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Efficiency of metaphase II oocytes following minimal/mild ovarian stimulation in vitro fertilization

Efficiency of metaphase II oocytes following minimal/mild ovarian stimulation in vitro fertilization

... Minimal and mild ovarian stimulation IVF usually refers to the use of low-dose gonadotropins with or without a sequential administration of clomiphene citrate [4–7]. Minimal/mild ovarian stimulation usually yields a ...

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Spontaneous antral follicle formation and metaphase II oocyte from a non-stimulated prepubertal ovarian tissue xenotransplant

Spontaneous antral follicle formation and metaphase II oocyte from a non-stimulated prepubertal ovarian tissue xenotransplant

... success in survival and development of frozen-thawed preantral follicles from prepubescent patients after xeno- transplantation in SCID mice and exogenous hormone stimulation. The result of finding a Met II oocyte ...

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How eggs arrest at metaphase II: MPF stabilisation plus APC/C inhibition equals Cytostatic Factor

How eggs arrest at metaphase II: MPF stabilisation plus APC/C inhibition equals Cytostatic Factor

... Many studies over several years have implicated the c-Mos/MEK/MAPK pathway in the metaphase arrest of the two most widely studied vertebrates, frog and mouse. Murine downstream components of this cascade are not ...

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Full term mouse development by abolishing Zn2+ dependent metaphase II arrest without Ca2+ release

Full term mouse development by abolishing Zn2+ dependent metaphase II arrest without Ca2+ release

... meiotic metaphase (mII) by the cytostatic factor Emi2, which sustains mII until fertilization by preventing the anaphase promoting complex (APC) E3 ubiquitin ligase from associating productively with its ...

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Reproductive_System.ppt

Reproductive_System.ppt

... secondary oocyte and the first polar body (when the follicle ruptures, ovulation is... Meiosis II : The secondary oocyte is frozen at metaphase II and will only continue the division [r] ...

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Clinical benefit of metaphase I oocytes

Clinical benefit of metaphase I oocytes

... Immature oocytes from superovulated cycles can undergo the final stages of meiotic maturation spontaneously in vitro. MI oocytes have already undergone the process of germinal vesicle breakdown (GVBD) and may progress to ...

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Using Fresh and Frozen Testicular Sperm Samples in Couples Undergoing ICSI-Micro TESE Treatment

Using Fresh and Frozen Testicular Sperm Samples in Couples Undergoing ICSI-Micro TESE Treatment

... Patients: A total of 82 consecutive IVF cycles were performed at the Fertijin IVF Center in Is- tanbul, Turkey from January 2010 to March 2012. This center has been approved by the Ministry of Health of Turkey and; ...

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Resolving RAD51C function in late stages of homologous recombination

Resolving RAD51C function in late stages of homologous recombination

... to metaphase II exhibited chromosomes with broken centromeres, but none showed any sister chroma- tid cohesion defect, which is consistent with the sexually dimorphic behavior of dicentric ...

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Preliminary Results of DHEA in Poor Responders in IVF

Preliminary Results of DHEA in Poor Responders in IVF

... retrieved, metaphase II oocytes, blastocysts and frozen embryos, clinical pregnancy rate and mis- carriage ...retrieved, metaphase II oocytes, blasto- cysts, frozen embryos and miscarriage ...

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Autosomal Trisomy and Triploidy Are Corrected During Female Meiosis in Caenorhabditis elegans

Autosomal Trisomy and Triploidy Are Corrected During Female Meiosis in Caenorhabditis elegans

... in metaphase II spindles of triploX worms or trisomy IV worms (Figure 6) indicates that univalent chromosomes in triploX or trisomy IV worms also remain intact during anaphase ...

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ROLE OF FIBRILLIN 3 ON FERTILIZATION CAPACITY OF INFERTILE WOMEN IN ICSI CYCLE

ROLE OF FIBRILLIN 3 ON FERTILIZATION CAPACITY OF INFERTILE WOMEN IN ICSI CYCLE

... Background: The growth and maturation of the oocyte and follicle are influenced by many paracrine and growth factors like fibrillin-3 hormone. However, there is lack of understanding its function and how interact in IVF ...

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Mapping EBNA-1 Domains Involved in Binding to Metaphase Chromosomes

Mapping EBNA-1 Domains Involved in Binding to Metaphase Chromosomes

... Strikingly, EBV-infected B-cell lines maintain relatively con- stant numbers of episomal copies for long periods (reviewed in reference 24), which reflects both the replication of the viral genomes and their efficient ...

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Spontaneous and Bleomycin Induced γH2AX Signals in CHO9 Metaphase Chromosomes

Spontaneous and Bleomycin Induced γH2AX Signals in CHO9 Metaphase Chromosomes

... BLM-treated metaphase spreads in order to characterize and compare γ H2AX signal distribution (per metaphases and chromosomes) in the absence (basal γ H2AX signals) and presence of DNA damage (BLM- induced γ H2AX ...

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5- Cell Cycle - PPT slides

5- Cell Cycle - PPT slides

... Create telophase & cytokinesis structure by making changes. to your metaphase structure.[r] ...

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Mouse Emi2 as a distinctive regulatory hub in second meiotic metaphase

Mouse Emi2 as a distinctive regulatory hub in second meiotic metaphase

... following parthenogenetic activation (see Fig. S8A,B in the supplementary material). CaM-dependent enzymes calcineurin (CaN, also called PP2B) and calmodulin kinase II (CaMKII) mediate Xenopus mII exit and it has ...

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DIFFERENTIAL X-RAY SENSITIVITY OF TRADESCANTIA CHROMOSOMES DURING THE MITOTIC CYCLE

DIFFERENTIAL X-RAY SENSITIVITY OF TRADESCANTIA CHROMOSOMES DURING THE MITOTIC CYCLE

... Range of Sensitivity.-On the basis of the above argument it is considered that the maximum chromosome breakage shown on the fifth day is a t the metaphase-anaphase [r] ...

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