Micrococcal nuclease
Contribution of H3K4 demethylase KDM5B to nucleosome organization in embryonic stem cells revealed by micrococcal nuclease sequencing
... of active genes in ES cells, where KDM5B focuses H3K4me3 near promoters to prevent it from spreading to gene bodies [18], an effort that may support tran- scriptional consistency by resetting H3K4 methylation after the ...
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Intracellular forms of simian virus 40 nucleoprotein complexes. IV. Micrococcal nuclease digestion.
... Virion assembly intermediates with different degrees of virion maturation showed intermediate resistance, and three products were found: nucleosomal DNA fragments, representing the fract[r] ...
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Intracellular DNA of the parvovirus minute virus of mice is organized in a minichromosome structure.
... Electrophoresis of DNA fragments pro- complexes can be leached from infected cell duced by micrococcal nuclease digestion of the DPC nuclei in the presence of hypotonic buffer 11, presen[r] ...
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Structural organization and polypeptide composition of the avian adenovirus core.
... a EcoRI restriction patterns of intact CELO virus DNA lane m and of CELO virus DNA extracted from viral cores that were digested with 1.5 U of micrococcal nuclease per ml for 0.5 min lan[r] ...
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Poly(rC) Binding Proteins and the 5′ Cloverleaf of Uncapped Poliovirus mRNA Function during De Novo Assembly of Polysomes
... or micrococcal nuclease- treated HeLa S10 ...coccal nuclease-treated cell extracts because competition with host mRNAs in untreated extracts could theoretically affect the ability of PV mRNA to ...
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Comparison of nuclease digestion of polyoma virus nucleoprotein complex and mouse chromatin.
... Lengths of DNA fragments obtained from digestion ofpolyoma and mouse chromatin with micrococcal nuclease" DNA fragment Length of DNA fragment base pairs Polyoma virions Mouse nuclei 0.3b[r] ...
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Nucleosome-like structural subunits of intranuclear parental adenovirus type 2 DNA.
... Recovery of 32P-labeled adenovirus 2 DNA and viral transcriptional activity in nuclei prepared for micrococcal nuclease digestion iii, as compared to crude nuclear preparations i, iia Vi[r] ...
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A segment of the 5' nontranslated region of encephalomyocarditis virus RNA directs internal entry of ribosomes during in vitro translation.
... The effect of the poliovirus 5'NTR on in vitro translation was compared with that of the 5'NTR of encephalomyocarditis virus by the use of synthetic mRNAs, micrococcal nuclease-treated H[r] ...
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TNFα signalling primes chromatin for NF κB binding and induces rapid and widespread nucleosome repositioning
... Results: Using micrococcal nuclease treatment and high-throughput sequencing, we map genome-wide changes in nucleosome positioning in primary human endothelial cells stimulated with tumo[r] ...
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Epigenetic Status of an Adenovirus Type 12 Transgenome upon Long-Term Cultivation in Hamster Cells
... The epigenetic status of integrated adenovirus type 12 (Ad12) DNA in hamster cells cultivated for about 4 decades has been investigated. Cell line TR12, a fibroblastic revertant of the Ad12-transformed epitheloid hamster ...
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Cleavage of phi X174 single-stranded DNA by gene A protein and formation of a tight protein-DNA complex.
... Less than 0.5% and 0.1% of the total 32p label remained resistant to DNase I and micrococcal nuclease digestion, respectively, measured as trichloroacetic acid-precipitable The introduct[r] ...
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Evidence that vesicular stomatitis virus produces double-stranded RNA that inhibits protein synthesis in a reticulocyte lysate.
... Effect of melting and micrococcal nuclease on the residual fractional capacity of polyA+ RNA from VSV-infected HeLa cells and polyI:polyC to inhibit protein synthesis by reticulocyte lys[r] ...
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Nucleosomal structure of Epstein-Barr virus DNA in transformed cell lines.
... Autoradiograms of DNA obtained from micrococcal nuclease digests of lymphoblastoid cell nuclei by using 32P-labeled Raji DNA as hybridization probe to detect all of the DNA present, both[r] ...
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Genome editing of oncogenes with ZFNs and TALENs: caveats in nuclease design
... Gene editing using ZFNs and TALENs has been effi- ciently used in many systems. The ability to utilize a cell’s endogenous repair system has made it possible to make alternations in the gene in a site-specific man- ner. ...
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Nuclease resistant synthetic ribozymes: developing a new class of therapeutics
... Nuclease-resistant synthetic ribozymes are now being developed for a number of therapeutically important tar- gets. For example, the antiangiogenic ribozyme ANGIOZYME, which targets the VEGF receptor Flt-1, has ...
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Genome Engineering of Drosophila with the CRISPR RNA-Guided Cas9 Nuclease
... CRISPR-associated nuclease (Cas9) to direct sequence-specific cleavage of exoge- nous ...common nuclease to specific DNA sequences by a short, readily generated ...chimeric nuclease for each ge- nome ...
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Evaluation of 5′ Nuclease Assay for Detection of Actinobacillus pleuropneumoniae
... Bacterial strains. A total of 203 Danish field isolates of A. pleuropneumoniae, 73 strains isolated from pigs with pleuropneumonia and 120 strains isolated from tonsils, representing serotypes 2 (n ⫽ 18), 5 (n ⫽ 16), 6 ...
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Gene 32 protein of bacteriophage T4 moderates the activities of the T4 gene 46/47-controlled nuclease and of the Escherichia coli RecBC nuclease in vivo.
... Trichloroacetic acid precipitation of 32P-labeled parental 0 and of 3H-labeled progeny DNA 0 of 32 single mutants and of gene 32/gene 46 double mutants in Rec+ [JC1557 in a and c and in [r] ...
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Exploration of Nitrogen Recycling in Algal Cultivation & Novel Methods of Plastid Genome Modification.
... a nuclease remain intact, they will continue to be ...a nuclease and synthetic NHEJ system will tend to create a heteroplasmic variety of mutations which are more stable than wild type ...a nuclease ...
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Tudor Nuclease Genes and Programmed DNA Rearrangements in Tetrahymena thermophila
... Tudor nuclease gene homologs, TTN1 and TTN2, in the ciliate Tetrahymena thermophila, which has two distinct small-RNA ...Tudor nuclease mutants revealed exceptionally high rates of deletion of the neo ...
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