Microfluidic tools for cell biological studies
A microfluidic flow-cell for the study of the ultrafast dynamics of biological systems
... Consequently, if the sample flow is insufficient, the quick ac- cumulation of closed RCs will result in the loss of transient signature from P 700 + . As shown in Figures 2(a1) and 2(a2), indeed when the cell-pump ...
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Microfluidic tools for connecting single-cell optical and gene expression phenotype
... a cell counting slide and visualized on the EVOS Cell Imaging System (Thermo Fisher) and monodispersity is calculated using ...a microfluidic flow-focusing device with a cross-sectional channel ...
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Development of microfluidic tools for cancer single cell encapsulation and proliferation in microdroplets
... current cell growth techniques in vitro and the influence of the cellular microenvironment in ...cancer cell growth. The goal of this thesis was to develop a set of microfluidic-based tools ...
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Cell Biological Studies of the Prion Protein
... the cell biology of PrP C and PrP Sc in neurons, astrocytes, lymphocytes, and other differentiated cell types that are relevant to prion biology, as opposed to the transformed cell lines that have ...
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Microfluidic chip calorimeters for biological applications
... Parylene microfluidic structures are unique in that they are thin-film structures with uniform ...for microfluidic biosensor ...as cell culture. To take advantage of Parylene for microfluidic ...
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Investigation of the heat shock response in yeast: quantitative modeling and single-cell microfluidic studies
... 2.4 Materials and methods This section will be used without any change in our manuscript for publication, which is under prepration at the moment (Zheng et al., ). Strains, plasmids and cell growth All ...
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Microfluidic Technology for the Generation of Cell Spheroids and Their Applications
... 3D cell cultures are cost effective and easy-to-use solutions for various ...The microfluidic technology evidently demonstrated its suitability for the translation of 3D cell spheroid technology into ...
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Microfluidic cell culture systems for drug research
... innovative microfluidic-based assays for cell ...conventional cell culture techniques, a microfluidic-based cell culture possesses several inherent advantages and can provide versatile ...
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Integrated Microfluidic Devices for Cell Culture and Assay
... the cell culture level with low shear ...of cell (10 µm) is around ...for microfluidic devices and is unavailable for conventional culturing containers such as flasks or well ...other cell ...
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Microfluidic Approaches for Cancer Cell Separation: Review
... and biological modifica- tions to functionalize the surface of beads with specific ...the cell membrane, A549 cancer cells spiked in RBCs were effectively captured and sorted on the microfluidic ...
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Microfluidic engineering of artificial stem cell niches
... stem cell niche, ECM proteins, growth factors, morphogens or cytokines can all act as biochemical cues, which can be present in a soluble or tethered ...stem cell self-renewal through STAT3 ...many ...
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Workflow tools for biological applications
... form, with rows and columns representing binned values for time and m/z ratio respectively. A single cell contains the intensity observed for a particular binning of time and m/z. At intial data collection, these ...
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Biophysical and Cell Biological Studies Characterizing the Vertebrate Iron Exporter Ferroportin
... prior studies, whereas others are in ...these studies were performed without cycloheximide pretreatment and using a lower hepcidin-25 concentration of ...
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Automated microfluidic cell culture of stem cell derived dopaminergic neurons
... automated cell culture to enable comprehensive phenotyping of large, parallel sets of personalised, in vitro, midbrain-specific, dopaminergic neuronal models of Parkinson’s ...the cell autonomous ...
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Towards microfluidic reactors for in situ synchrotron infrared studies
... silicon microfluidic reactors therefore allows an unprecedented amount of control over reaction parameters whilst decreasing compromises made in spectroscopic cell ...for microfluidic devices in ...
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Advances in three-dimensional rapid prototyping of microfluidic devices for biological applications
... the cell culture insert as it has a rugged base, which supports the membrane and can be easily discarded after ...disposable cell inserts offers a supportive platform for a reusable fluidic ...
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Microfluidic platforms for the investigation of fuel cell catalysts and electrodes
... structural studies can now be performed using microfluidic fuel cells and MicroCT ...initial studies, the effects of compression on the structure and performance of acidic fuel cell cathodes ...
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Design and validation of a microfluidic device for controlled cell stimulation
... Synopsis Microfluidic devices promise to enhance fundamental biological research by offering precise, consistent, and easily adjustable experimental ...a microfluidic device that can deliver chemical ...
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A digital microfluidic platform for primary cell culture and analysis
... immortalized cell lines and primary cells. Immortalized cell lines can be grown in vitro for many genera- tions, spanning many ...long-term studies involving animal models of disease, primary cells ...
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Chemical Tools for the Control of Biological Systems
... The Xenopus laevis model system has been used extensively in the Dougherty lab to study ion channels containing noncanonical amino acids. Introduced via a chemical acylation methodology, these noncanonical amino acids ...
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