MicroRNA array, mRNA sequencing and integrative analysis
Identification of tumor suppressor miRNAs by integrative miRNA and mRNA sequencing of matched tumor–normal samples in lung adenocarcinoma
... an integrative analysis of miRNA and mRNA sequencing data for matched tumor–normal samples from 109 Korean female patients with non-small-cell lung adenocarcinoma ...miRNA sequencing ...
13
Integrated mRNA and microRNA transcriptome sequencing characterizes sequence variants and mRNA–microRNA regulatory network in nasopharyngeal carcinoma model systems
... Fig. 6. Meta-analysis of NGS data with clinical data from public database. (A) Meta-analysis on GSE12452 , GSE13597 and NGS data; (B)meta-analysis on GSE34573 and NGS data; Dotted line: threshold for ...
13
Integrative network-based analysis of mRNA and microRNA expression in 1,25-dihydroxyvitamin D3-treated cancer cells
... There are many different network algorithms that can be used to investigate the vitamin D-extended network. In our analysis, we selected two different approaches to demon- strate the power of network ...
12
Integrative analysis of the microRNA-mRNA response to radiochemotherapy in primary head and neck squamous cell carcinoma cells
... systems analysis of HNSCC cells in response to additional inhibitors and perturbations be- comes ...miRNA analysis revealed many miRNAs regulating immune re- sponse and inflammatory molecules deregulated in ...
16
Original Article Down-regulation of microRNA-20a in gemcitabine-resistant pancreatic cancer in in vitro microRNA integrative analysis
... MiRNAs microarrays Total RNAs were extracted using Trizol reagent (Takara Biotechnology, Dalian, China). Micro- array analysis was performed according to the manufacturer’s protocol. 5 μg of total RNA from ...
9
Characterization of a MicroRNA Harboring Intron for Pre-mRNA Splicing and MicroRNA Processing
... All splice site mutants along with wild type minigene construct and appropriate controls were transfected in CHO cells using polybrene and upon RNA isolation analyzed for spliced phenotypes. Wild type (WT) spliced ...
89
Super-Enhancer-Mediated RNA Processing Revealed by Integrative MicroRNA Network Analysis
... ChIP analysis of DGCR8 and Drosha revealed that deletion of SE constituents attenuated recruitment of these two proteins to pri-miRNA loci in three cell types in accordance with the reduced efficiency of pri-miRNA ...
35
Integrated analysis of microRNA regulatory network in nasopharyngeal carcinoma with deep sequencing
... Results: Eight differentially expressed miRNAs were identified between NPC and chronic nasopharyngitis patients by deep sequencing. Further qRT-PCR assays confirmed 3 down-regulated miRNAs (miR-34c-5p, miR-375 and ...
11
miRTarVis: an interactive visual analysis tool for microRNA mRNA expression profile data
... exploratory analysis of miRNA-mRNA expression profile ...visual analysis tools for miRNA- mRNA expression profile ...and mRNA expression datasets are multidimensional, searching for ...
16
Integrative transcriptome analysis identifies deregulated microRNA-transcription factor networks in lung adenocarcinoma
... miRNA sequencing of lung adenocarcinoma from Brazilian patients, extending to validation in an independent sample set as well as across multiple high-throughput miRNA and gene expression ...data analysis, ...
15
Prediction of functional microRNA targets by integrative modeling of microRNA binding and target expression data
... down mRNA transcripts that were associated with the miRISC in mouse ...throughput sequencing was then performed to identify these mRNA transcript tags, ...
10
Deep Sequencing the MicroRNA Transcriptome in Colorectal Cancer
... based array technologies and quantitative RT-PCR (qRT-PCR) ...Deep sequencing has emerged as an attractive approach for global miRNA analysis, advantages including pooling of samples for ...
12
MicroRNA and mRNA Processing Variations in Normal and Hypertrophic Hearts
... Our sequencing, qPCR, and Western blot data of specific mRNA 3’UTR factors add to this wealth of knowledge, although the evidence for this is as yet ...This analysis would establish whether there is ...
186
MicroRNA/mRNA profiling and regulatory network of intracranial aneurysm
... Integrated analysis of misregulated miRNAs and mRNAs miRNAs modulate gene expression through both mRNA degradation and translational repression mechanisms, and miRNA-mRNA regulatory networks are ...
10
Integrative bioinformatics analysis characterizing the role of EDC3 in mRNA decay and its association to intellectual disability
... transcription, mRNA processing, translation, and deg- radation ...tic mRNA decay pathways, the 5′ to 3′ and the 3′ to 5’ mRNA decay, both start with the deadenylation of the 3′ end poly(A) tail [3, ...
11
Integrative Pathway Analysis Pipeline For Mirna And Mrna Data
... pathway analysis using just miRNA expression, such as mirTar [64, 65] and DIANA-miRPath ...both mRNA and miRNA for pathway analysis [19, ...implements mRNA-miRNA integration is the miRNA and ...
109
Identification of body fluids by mRNA analysis with minion nanopore sequencing
... DNA analysis to identify the individual who contributed the body ...DNA analysis results allows investigators to link a particular individual to a crime scene and presents information that gives them a ...
73
Processing of Agilent microRNA array data
... Results and Discussion We compared four methods for obtaining a processed microRNA signal that can be used for statistical microar- ray data analysis. The four processed signals analyzed were a) the total ...
6
Analysis of microRNA profile of Anopheles sinensis by deep sequencing and bioinformatic approaches
... Results: We identified 49 conserved miRNAs and 12 novel miRNAs by next-generation high-throughput sequencing technology. In contrast, 43 miRNAs were predicted by the bioinformatic approach, of which two were ...
12
BioVLAB MMIA: A Reconfigurable Cloud Computing Environment for microRNA and mRNA Integrated Analysis
... E. Input and output for the analysis using BioVLAB-MMIA BioVLAB-MMIA provides users a way to upload miRNA expression data via Amazon S3 Interface. The result re- ports are also publicly available on Amazon S3 ...
6