Migration Assay
Ring cell migration assay identifies distinct effects of extracellular matrix proteins on cancer cell migration
... cell migration can distinguish dif- ferences in cell motility among different cell lines, two sets of cancer cells, ...this assay and compared to HeLa ...cell migration assay as de- scribed ...
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Inhibition of cancer cell migration with CuS@mSiO<sub>2</sub>-PEG nanoparticles by repressing MMP-2/MMP-9 expression
... cell migration in vitro. A transwell-based migration assay was performed to quantitatively evaluate the migration after stimulation with CuS@SiO 2 ...transwell-based migration ...
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Legumain Promotes Gastric Cancer Progression Through Tumor-associated Macrophages In vitro and In vivo
... CCK-8 assay and transwell migration assay indicated that upregulation of LGMN expression in TAMs stimulated cell proliferation, migration and invasion in vitro, while downregulation of LGMN ...
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Methylation-mediated repression of microRNA-129-2 suppresses cell aggressiveness by inhibiting high mobility group box 1 in human hepatocellular carcinoma
... healing assay and Transwell migration assay revealed that exogenous expression of miR-129-2 dramatically inhibited cell migration in comparison with that of control cells (P < ...invasion ...
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LncRNA BCAR4, targeting to miR-665/STAT3 signaling, maintains cancer stem cells stemness and promotes tumorigenicity in colorectal cancer
... knock down of BCAR4 impaired CSCs stemness (Fig. 3b). Then migration assay indicated inhibition of BCAR4 impaired the migration capacity of ALDH+ cells rather than ALDH+ CRC cells. Consistently, ...
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Anticancer and Anti-metastatic Effects of Supercritical Extracts of Hops (Humulus lupulus L.) and Mango ginger (Curcuma amada Roxb.) in Human Glioblastoma
... and ethanol extract of hops (HL) in the U-87MG human glioblastoma cell line. Both CA and HL individually demonstrate strong cytotoxicity against glioblastom a cells. CompuSyn analysis of cytotoxicity data confirms that ...
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Original Article Sox3 silencing inhibits metastasis and growth of esophageal squamous cell carcinoma cell via down-regulating GSK-3β
... ous biological factors on the malignant poten- tial of ESCC [20]. Thus, the identification of a marker that predicts the metastasis, and hence prognosis, is highly desirable. In the present study, we identified the role ...
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The inhibition role of miR-22 in hepatocellular carcinoma cell migration and invasion via targeting CD147
... per assay. The migration assay is the same with invasion assay excepting no matrigel was used and the permeating time for cells was 12 ...
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Aphthous ulcer drug inhibits prostate tumor metastasis by targeting IKKɛ/TBK1/NF-κB signaling
... reporter assay and immunoblotting. Wound-healing assay, sphere formation, transwell migration assay, and in vivo intracardiac and orthotopic tumor xenograft experiments were used to evaluate ...
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Bioassay-Guided Fractionation of Wound Healing Active Compounds From Piper nigrum L. Berries Extract in Malaysia
... The study aimed to further evaluate the wound healing property of P. nigrum L. using bioassay-guided fractionation method. The ethanolic extract of Piper nigrum L. was fractionated in two stages using column ...
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Original Article Effect of interleukin-6 on invasion and migration of hepatic carcinoma cells
... and migration is associated with the change in cell adhesion, degradation of extracellular matrix and the morphological changes, and all these processes are complet- ed via constantly changing cytoskeletons ...and ...
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Original Article Amygdalin-mediated inhibition of non-small cell lung cancer cell invasion in vitro
... conditions. In contrast, the tumor cells often spontaneously acquire the moving ability and break away from normal physiological regula- tion. Integrin is a protein containing multi-sub- units including α and β-subunits, ...
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RND3 promotes Snail 1 protein degradation and inhibits glioblastoma cell migration and invasion
... However, emerging evidence has suggested that other regulatory signaling pathways are also involved in RND3-mediated regulations. In esophageal squamous cells, forced expression of RND3 suppresses cell migration ...
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<p>miR-22 Suppresses Tumor Invasion and Metastasis in Colorectal Cancer by Targeting NLRP3</p>
... NLRP3 has been reported to be involved in the metastasis of many tumor types. The expression of NLRP3 in HCT116 cells was detected by quantitative RT-PCR (qRT-PCR) and Western blotting (Figure 3A and B). The expression ...
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Exogenous carbon monoxide inhibits neutrophil infiltration in LPS-induced sepsis by interfering with FPR1 via p38 MAPK but not GRK2
... neutrophil migration is not known. Under agarose migration assays were performed, and the potent chemotactic ligand to FPR1 (fMLP) was used as a chemoattractant to further determine whether LPS promoted ...
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Exosomes from human umbilical cord blood accelerate cutaneous wound healing through miR-21-3p-mediated promotion of angiogenesis and fibroblast function
... and migration are essential for wound contraction, collagen synthesis and tissue remodeling ...and migration, suggesting that the activation of fibroblasts is a mechanism through which UCB-Exos stimulate ...
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Original Article MiR-152 regulates metastases of non-small cell lung cancer cells by targeting neuropilin-1
... mor suppressor that targets TGFα in prostate cancer [20]. However, the role of miR-152 in the NSCLC cell metastasis remains unknown. Neuropilins, including neuropilin-1 and neuropi- lin-2, are multifunctional non ...
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Long noncoding RNA MIR31HG inhibits hepatocellular carcinoma proliferation and metastasis by sponging microRNA-575 to modulate ST7L expression
... qRT-PCR assay also determined the similar result in HCC cells, suggesting that MIR31HG might play a role in posttranscriptional ...reporter assay verified our supposition that miR-575 binds to MIR31HG in a ...
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RNAi silencing of c-Myc inhibits cell migration, invasion, and proliferation in HepG2 human hepatocellular carcinoma cell line: c-Myc silencing in hepatocellular carcinoma cell
... The in vitro invasion assay was designed to test whether transfection of siRNA c-Myc altered the in- vasive potential of tumor cells in Matrigel-coated transwell chamber. Considering as 100% in the un- treated ...
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By downregulating TIAM1 expression, microRNA-329 suppresses gastric cancer invasion and growth
... Gastric cancer (GC) is one of the most common malignant tumors worldwide. Emerging evidence has shown that abnormal microRNAs (miRNAs) expression is involved in tumorigenesis. MiR-329 was previously reported to act as a ...
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