minor groove binder
Quantification of Intestinal Bacterial Populations by Real Time PCR with a Universal Primer Set and Minor Groove Binder Probes: a Global Approach to the Enteric Flora
... used. A nonfluorescent quencher is essentially a chromophore that acts as an energy transfer acceptor from the reporter molecule that does not emit a detectable fluorescent signal of its own, giving a less complicated ...
7
Establishment of a minor groove binder probe based quantitative real time PCR to detect Borrelia burgdorferi sensu lato and differentiation of Borrelia spielmanii by osp A specific conventional PCR
... Background: Borrelia burgdorferi sensu lato (sl), the causative agent of Lyme borreliosis, is transmitted by ticks of the genus Ixodes as vector. For identification of Borrelia infections in ticks a TaqMan™ minor ...
10
Development of a highly sensitive real time one step RT PCR combined complementary locked primer technology and conjugated minor groove binder probe
... This study was developed and validated a rapid, sensi- tive, and reliable real-time RT-PCR assay for the routine identification of EVs using the TaqMan minor groove binder (MGB) format combined ...
6
Detection of a Point Mutation Associated with High Level Isoniazid Resistance in Mycobacterium tuberculosis by Using Real Time PCR Technology with 3′ Minor Groove Binder DNA Probes
... -minor groove binder-DNA probe), was developed to detect either the wild-type or the mutant codon directly in Ziehl-Neelsen-positive sputum ...
6
Real Time PCR Based Pathotyping of Newcastle Disease Virus by Use of TaqMan Minor Groove Binder Probes
... nonfluorescent minor groove binder (MGB) quencher amplified and hybridized to a region in the fusion protein (F) gene that corresponds to the cleavage site of the F0 precursor, which is a key ...
10
Sensitive Assay for Quantification of Hepatitis B Virus Mutants by Use of a Minor Groove Binder Probe and Peptide Nucleic Acids
... a minor component of the mixed ...a minor strain is difficult as long as a matching reaction with a single probe is included in the ...using minor groove binder probes and peptide ...
8
Smallpox and pan Orthopox Virus Detection by Real Time 3′ Minor Groove Binder TaqMan Assays on the Roche LightCycler and the Cepheid Smart Cycler Platforms
... We designed, optimized, and extensively tested several sensitive and specific real-time PCR assays for rapid detection of both smallpox and pan-orthopox virus DNAs. The assays are based on TaqMan 3 ⴕ -minor ...
9
A simplified duplex real time PCR incorporating TaqMan minor groove binder (MGB) probes and an exogenous internal positive control for the simultaneous detection of Mycoplasma gallisepticum and Mycoplasma synoviae cultures
... labelled minor groove binder (MGB) probes that target the cytadhesin encoding surface protein (mgc2) gene and the haemagglutinin surface protein (vlhA) gene of MG and MS, ...
6
Detection of Norovirus genogroup I and II by multiplex real time RT PCR using a 3' minor groove binder DNA probe
... Europe [3,4]. Hospitals, residential facilities/nursing homes and schools/nurseries were most frequently affected. To prevent further spreading of the causative agent during a mass outbreak especially in semi closed ...
6
Detection of the Bacillus anthracis gyrA Gene by Using a Minor Groove Binder Probe
... Identification of chromosomal markers for rapid detection of Bacillus anthracis is difficult because significant chromosomal homology exists among B. anthracis, Bacillus cereus, and Bacillus thuringiensis. We evaluated ...
7
An evaluation of minor groove binders as anti-lung cancer therapeutics
... Minor groove binders that have seen use previously as anti-cancer agents are often modified with an DNA-alkylating moiety, such as in ...of minor groove binder. In the case of other ...
11
Distinguishing Between Two Closely Related Strains of Xylella fastidiosa
... Taqman minor groove binder (MGB) probes was developed to quantify and differentiate two strains of Xylella fastidiosa, Pierce’s Disease (PD) strain and Almond Leaf Scorch (ALS) ...
6
Comparative effects of A.T and G.C sequence selective DNA minor groove binding agents
... in minor groove binder-DNA interactions is the 'phasing' of the bond forming moieties of the ligand and the DNA (Lown, ...the minor groove of DNA (Goodsell & Dickerson, ...
227
Design, synthesis and antibacterial activity of minor groove binders: the role of non-cationic tail groups
... of minor groove binder (MGBs) in which, the cationic tail group has been replaced by a neutral, polar variant including cyanoguanidine, nitroalkene, and trifluoroacetamide ...a minor ...
9
← Return to Article Details A PROMISING DNA GROOVE BINDER AND PHOTOCLEAVER BASED ON A DINUCLEAR RUTHENIUM(II) COMPLEX
... the groove binding or intercalating of the complex to DNA, thus we attributed the DNA viscosity behaviors in the presence of the complex to groove binding mode, which is similar to the reported DNA ...
7
Sequence-selective minor groove recognition of a DNA duplex containing synthetic genetic components
... impact minor groove ...other minor groove binders such as Hoechst33258 in complex with A-tract dsDNA ...uences groove width but also reduces allosteric modulation in a DNA ...of ...
9
Four pyrrole derivatives used as building blocks in the synthesis of minor groove binders
... amido)-N-[2-(morpholin-4-yl)ethyl]-1H-pyrrole-2-carboxamide}, are inter- mediates used in the synthesis of modified DNA minor-groove binders. In all four compounds, the nitro groups lie in the plane of the ...
24
Rationalising sequence selection by ligand assemblies in the DNA minor groove : the case for thiazotropsin A
... Selective recognition of short sequence nucleic acid targets or larger transcription factor binding sites by sequence reading, minor groove binding small molecules (MGBs) remains rela- tively difficult, ...
12
Four pyrrole derivatives used as building blocks in the synthesis of minor-groove binders
... amido)-N-[2-(morpholin-4-yl)ethyl]-1H-pyrrole-2-carboxamide}, are inter- mediates used in the synthesis of modified DNA minor-groove binders. In all four compounds, the nitro groups lie in the plane of the ...
25
Repair of DNA interstrand cross-links induced by anticancer agents in mammalian cells
... DSBs are repaired by means to two main mechanisms: homologous recombination (HR) and non-homologous end-joining (NHEJ). Both mechanisms operate in all eukaryotes, but their relative contributions differ between higher ...
242