10 mM sodium phosphate buffer, 0.15 M NaCl, pH 7.4
Elucidation of membrane stabilizing Potentials of Methanolic Extract of Vigna unguiculata (cowpea) Linn (seed)
... Whole blood was collected from male human under standard condition. EDTA was used to prevent clotting. The blood was washed three times with isotonic solution (154 mMNaCl) in 10 mM sodium ...
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ANTIOXIDANT ASSAY IN VIVO AND VITRO
... from sodium nitroprusside and measured by the Griess reaction13. Sodium nitroprusside in aqueous solution at physiological pH spontaneously generates nitric oxide, which interacts with oxygen to ...
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Electrooxidation of Catechols in the Presence of Sulfite: Presentation of a Facile and Green Method for Aromatic Sulfonation
... of 4-tert-butylcatechol ( 1a ) in the presence of sulfite ( 3 ) as nucleophile in aqueous solution containing ...0.2 M phosphate buffer (pH ...1.0 mM solution of 1a in the ...
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Heterologous expression of Pycnoporus cinnabarinus cellobiose dehydrogenase in Pichia pastoris and involvement in saccharification processes
... loading buffer as described above without heating; they were incubated at ambient temperature for 15 min and the gel was ...at 4°C, rinsed with deionized water and incubated for 2 h at 25°C in 50 ...
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L form like colonies of Staphylococcus aureus induced by an extracellular lytic enzyme from Pseudomonas aeruginosa
... and viable cells at pH 8.5 containing 10 mM sodium phosphate buffer and 0.25% heat-inactivated horse serum A and in the same medium to which 1 M sucrose was added A.. In both experiments[r] ...
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Dissociation of polyoma virus by the chelation of calcium ions found associated with purified virions.
... After adding virus to the dissociation shows the dissociated virions after a 15-min mixture 10 mM EGTA, 3 mM DTT, and 0.15 M NaCl in 0.01 M Tris buffer, pH 8.5, portions were exposure to[r] ...
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Ultrasensitive Biosensor for the Detection of Vibrio cholerae DNA with Polystyrene co acrylic Acid Composite Nanospheres
... 0.1 M of potassium phosphate buffer (pH 5) containing carbodiimide cross-linking reagents, ...0.002 M of EDC and 0.005 M of NHS for 2 h [32] before soaking overnight in ...0.05 ...
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Comparative evaluation of extraction methods for apoplastic proteins from maize leaves
... of 10 μg was separated by SDS-PAGE according to Laemmli ...8 M urea, 2 M thiourea, 0.5% IPG (immobilized pH gradient) buf- fer, 4% w/v CHAPS, 30 mM DTT, 20 mM Tris and ...
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Overcoming Diminished Motivation
... and 4, and ...(10 mM sodium phosphate buffer, pH ...and 10 mM sodium phosphate buffer (pH ...and 15 µM bp CT-DNA were used ...
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Use of a Novel Enzyme Immunoassay Based on Detection of Circulating Antigen in Serum for Diagnosis of Helicobacter pylori Infection
... to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS- PAGE), at 50 g/lane, using vertical slabs of 12 or 16% polyacrylamide ...0.05 M Tris buffer containing 200 mM NaCl ...
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The Role of Nonhemoglobin Proteins and Reduced Glutathione in the Protection of Hemoglobin from Oxidation In Vitro
... The flasks containing whole hemolysate and those containing hemoglobin solution also contained EDTA, 3.42 X 10-4 M, added in 0.15 M sodium chloride; 2 ml of 0.4 M phosphate buffer pH 7.4[r] ...
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Synthesis, Characterization, Electrocatalytic and Catalytic Activity of Thermally Generated Polymer-Stabilized Metal Nanoparticles
... Intense investigation and research in nanoparticle synthesis in the past decade can be traced to the realization that exploitation of nanoparticles for device fabrication requires the formation of morphologically ...
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Electrochemical Detection of Hydrogen Peroxide Based on Graphene Oxide/Prussian Blue Modified Glassy Carbon Electrode
... based on nanomaterial, such as metal nanoparticles, carbon nanotubes; metal oxides have been reported [14-15]. To exercise and confirm the functionalities of graphene, much attention has been on the fabrication of ...
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Tissues and Plasma Drug Concentrations Following Intraperitoneal Administration of Amphotericin B Prepared in Acetate/Acetic Acid Buffer Solution
... sonicated under 90% amplitude for 15 s, all in a glacial water bath. The homogenate was then centrifuged at 3500 g for 30 min. After re-extraction of the pellet, the supernatants were pooled and the final volume ...
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Quantitation of glutathione S-transferases in rice (Oryza sativa L.) roots exposed to cadmium by liquid chromatography–tandem mass spectrometry using isotope-labeled wing peptides as an internal standard
... (Fig. 4), when they were spiked into the mobile phase, indicating that using the internal standard peptides containing natu- ral flanking sequences around the cleavage sites improves quantitative ...
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Designing of Diclofenac Sodium Biodegradable Drug Implant for Speedy Fracture Healing
... A Petri-dish containing Formaldehyde solution (37% v/v) was placed in an empty glass dessicator. A wire mesh contained the implants was kept on the top of the Petri dish and the dessicator was closed immediately. The ...
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Highly Sensitive Electrochemical Determination of Dopamine with an Overoxidized Polypyrrole Nanofiber Pencil Graphite Electrode
... performance liquid chromatography (HPLC)-mass spectrometry [7], fluorometry [8], and electrochemistry [9]. Electrochemistry have received considerable attention in DA sensing, as a result of its rapid detection, ...
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The Induction of Proteolysis in Purulent Sputum by Iodides
... For example, we have demonnate were then mixed with equal volumes of strated effective liquefaction of whole purulent 0.05 M sodium phosphate buffer, pH 7.5, to sputum by the addition of[r] ...
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SODIUM CHLORIDE AND CADMIUM INDUCED OXIDATIVE STRESS AND ANTIOXIDANT RESPONSE IN CALAMUS TENUIS LEAVES
... (0.1 M phosphate buffer, pH 6.8) with mortar and pestle. The homogenate was then centrifuged at 15,000 g for 20 min and the homogenate was used as the crude extract for the assay of superoxide ...
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Study of the Effect of NaCl Addition to the PEM on the Functional Properties of BMJ24 Jeewanu Prepared under Oxygenic Conditions (PUOC) in Water and in Phosphate Buffer of pH 6, 7 and 8 under Anoxygenic Conditions.
... giving 4 hours exposure ...After 4 hours, 8 hours, 12 hours, 16 hours, 20 hours and 24 hours of exposure, the pH and the colorimetric readings of flask 1 and 2 were ...
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