molecular cloning
MOLECULAR CLONING OF OVINE cDNA LEPTIN GENE
... Molecular cloning refers to the procedure of isolating a defined DNA sequence and obtaining multiple copies of it in ...the cloning of PCR ...
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Molecular cloning of a functional Fads2 promoter from Zebrafish
... the cloning and functional characterization of a ...to molecular cloning, bioinformatics analysis revealed conserved modules of cis-elements within the fads2 promoter as compared to the ...
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Molecular cloning and partial sequencing of hepatitis A viral cDNA.
... Molecular cloning of foot and mouth disease virus genome and Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest the cloned sequences were HAV in origin, not from other seq[r] ...
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Molecular cloning of an enhancer binding protein.pdf
... From a series of screens of protein replica filters generated from platings of the ~,EB phage, the following conclusions were drawn: the specific detection of ZEB plaques requ[r] ...
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Characterization of the guinea pig cytomegalovirus genome by molecular cloning and physical mapping.
... Gel-purified fragments were recleaved with the same restriction endonuclease used initially, placed again in a large well on a second agarose gel, reisolated, and used to prepare in vitr[r] ...
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Molecular cloning and characterization of recombinant parasite antigens for immunodiagnosis of onchocerciasis
... OC 3.6 produced a stable fusion protein that was recognized by antibodies in 54 of 55 sera from onchocerciasis patients tested.. We have also found that this protein was recognized by se[r] ...
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Molecular cloning, expression and metabolic activity assay of CYP3A46 gene
... Identification of recombinant expressing cells by RT-PCR All operations carried out by the methods 1.4.2 herein Western-blot detection of expressing products in HepG2-CYP3A46 culturing a[r] ...
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Molecular cloning and characterization of symbiotic' genes from Rhizobium trifolii
... 20 nitrogenase and leghaemoglobin, no gene products required specifically for the nodulation process between Rhizo b ium and legumes have been identified and no "symbiotic" genes have be[r] ...
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Molecular Cloning, Expression And Purification Studies With An ORF Of Mycobacterium Tuberculosis
... Abstract: The study was initiated to develop a recombinant strain, for expression and production of large scale protein and to develop its purification protocol. The MRA_ORF-X was amplified from the genomic DNA of M. ...
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Molecular cloning and characterization of a nuclear androgen receptor activated by 11-ketotestosterone
... Two liver epithelial cell lines were used to test receptor activity. HepG2 cells (ATCC, Manassas, VA) were main- tained in DMEM supplemented with 10% FCS and non- essential amino acids. ZFL cells (ATCC) were maintained ...
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Molecular cloning and duplication of the nematode sex-determining gene tra-1.
... A congenic strain, containing about 5 cM of Bergerac-derived DNA around tra-I, but otherwise isogenic with N2, was constructed by repeated back-crossing (see Mate- r[r] ...
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Molecular cloning and expression of a murine homolog of the human poliovirus receptor gene.
... A murine cDNA probe containing sequences encoding extracellular domains 2 and 3, the transmembrane region, and the NH2terminal half of the cytoplasmic tail hybridized with 2- and 3-kb mR[r] ...
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Molecular cloning, characterization, and expression of the Tipula iridescent virus capsid gene.
... Primer extension analysis of the TIV capsid transcript indicated a putative transcription start site within a region about 19 nt upstream of the first ATG of the capsid ORF.. A second pr[r] ...
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Molecular cloning and epitope analysis of the peanut allergen Ara h 3
... These peptides were probed with a pool of serum IgE from patients who had previously recognized the wild-type peptide, to determine whether amino acid changes affected peanut-specific Ig[r] ...
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Molecular cloning of covalently closed circular DNA of bovine leukemia virus.
... Comprehensive restriction endonuclease mapping of the unintegrated bovine leukemia virus and the cloned DNA molecules showed that eight of the nine clones carried viral information witho[r] ...
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Molecular cloning and characterization of the RNA packaging-defective retrovirus SE21Q1b.
... To determine the specificity of RNA packaging of virus derived from the SE21Q1b molecular clone and the SE2lQlb/ASLV hybrid provirus constructs, viral particles were harvested from SE21Q[r] ...
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Molecular cloning and function of ecdysis triggering hormones in the silkworm Bombyx mori
... Isolation and identification of Inka cell peptide hormones Injection of epitracheal-gland extracts from pharate larval, pupal or adult stages into pharate 5th instar larvae induced stron[r] ...
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Molecular cloning of a family of retroviral sequences found in chimpanzee but not human DNA.
... Preliminary extested this hypothesis by cloning from chimpan- periments using probes to specific regions of the CPC1 genome identified two major EcoRI fragments in zee cellular DNA seque[r] ...
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Molecular cloning of a highly leukemogenic, ecotropic retrovirus from an AKR mouse.
... To confirm that the XAKR-623 clone encoded non-leukemogenic Akv, virus derived by transfection of cloned Akv DNA onto NIH 3T3 cells was also injected into newborn AKR/J and C3Hf/Bi mice [r] ...
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Molecular cloning and characterization of avian sarcoma virus circular DNA molecules.
... Four different cloned viral DNA molecules were isolated: SRA-1 contains two copies of the 330-base pair terminal redundancy normally found at each end of the linear DNA molecules, but ha[r] ...
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