monolayer culture
Sorting out of limb bud cells in monolayer culture
... Int J Ve\ lliol 38 351 356 (199~) 351 Sorting out of limb bud cells in monolayer culture NAOYUKI WADA* and HIROYUKIIDE Biological Institute, Tohoku University, Sendai, Japan ABSTRACT To examine differ[.] ...
6
Induction of neural crest cells from mouse embryonic stem cells in a serum free monolayer culture
... ABSTRACT The neural crest (NC) is a group of cells located in the neural folds at the boundary between the neural and epidermal ectoderm. NC cells differentiate into a vast range of cells, including neural cells, smooth ...
9
Chondrogenic re-differentiation potential of chondrocytes after monolayer culture: Comparison between osteoarthritis and young adult patients
... during monolayer culture and the expression of CD105 was higher in OAC than non-OAC in passage ...during monolayer culture, and OAC had higher chondrogenic differentiation than ...
8
DEPTOR has growth suppression activity against pancreatic cancer cells
... cell culture settings [1, 2], it has not been previously tested whether and how DEPTOR plays a role in pancreatic ...in monolayer culture and in soft agar, and decreased clonogenic survival by ...
9
Monolayer to MTS : using SEM, HIM, TEM and SERS to compare morphology, nanosensor uptake and redox potential in MCF7 cells
... Cellular redox potential is important for the control and regulation of a vast number of processes occurring in cells. When the fine redox potential balance within cells is disturbed it can have serious consequences such ...
10
Neurocytoma a Rare Intraventricular Tumor
... using monolayer culture for first few passages and then a portion of each specimen was cultured in collage gel (1) Immunostaining for synaptopysin or glial fibrillary acidic protein performed on a primary ...
8
Antibiotics inhibit sphere-forming ability in suspension culture
... suspension culture was severely impacted by P/S addition that triggered about fivefold decrease in sphere formation ...in monolayer culture ...in monolayer culture- led us to think that ...
9
Genetic Analysis of the Human Papillomavirus Type 31 Differentiation-Dependent Late Promoter
... Fragment A contains not only p742 but also p99, the major viral early promoter (46). It is likely that p99 also contributes to reporter activity in the context of these constructs. To test this possibility, we created ...
13
Human disc cells in monolayer vs 3D culture: cell shape, division and matrix formation
... Cell Culture Methods: Primary cultures were grown as previously described ...primary culture, cells were subsequently passaged in either monolayer culture (seeded at 5,000 cells/well of a ...
6
A unique ectonucleotide pyrophosphohydrolase associated with porcine chondrocyte derived vesicles
... Anti-SNTP in a dilution of 1:7,000 was used in NWestern blots against 1% SDS extracts from each of the following : a porcine chondrocytes in monolayer culture harvested and washed Itwice[r] ...
7
Transforming growth factor beta1 induces differentiation in human papillomavirus-positive keratinocytes.
... in monolayer culture whether our immortalized human keratinocytes were sensitive to the inhibitory effects of TGF- b 1 on cellular prolif- ...in monolayer cultures could be correlated with induction ...
12
The Development of Non Viral Gene Activated Matrices for Bone Regeneration Using Polyethyleneimine (PEI) and Collagen Based Scaffolds
... The healing potential of scaffolds for tissue engineering can be enhanced by combining them with genes to produce gene-activated matrices (GAMs) for tissue regeneration. We examined the potential of using ...
21
Fibroblasts derived from human embryonic stem cells direct development and repair of 3D human skin equivalents
... Results: While both EDK and H9-MSC cell lines exhibited similar morphology and mesenchymal cell marker expression, they demonstrated distinct functional properties when incorporated into the stromal compartment of HSEs. ...
12
Tendon proper- and peritenon-derived progenitor cells have unique tenogenic properties
... in culture, total RNA was extracted from tendon proper- and peritenon-derived progenitor- seeded constructs by mechanically homogenizing tissues with a Tissue-Tearor (Biospec Products, ...in monolayer ...
15
Original Article Insulin-like growth factor-I maintains the phenotype of rat endplate chondrocytes in vitro via distinct signaling pathways
... and culture in vitro as a monolay- er, they also lose their phenotype and various senescence and de-differentiation-mediating genes are expressed ...in monolayer culture, the expression of COL- 2A ...
10
Human infrapatellar fat pad derived stem cells express the pericyte marker 3G5 and show enhanced chondrogenesis after expansion in fibroblast growth factor 2
... to culture conditions and not the reminis- cence and the demonstration of a cell origin, and further work is needed before any firm conclusions are ...initial culture on plastic and with pas- sage, ...
11
Improved screening procedure for biogenic amine production by lactic acid bacteria and Enterobacteria
... Enterobacteria). Monolayer culture and double layer colour development methods for the detection of amino acid decarboxylase-positive lactic acid bacteria and Enterobacteria were ...
8
Properties and usefulness of aggregates of synovial mesenchymal stem cells as a source for cartilage regeneration
... a monolayer culture, aggregates of human synovial MSCs cultured for 1, 2 and 3 days, and the pellets cultured for 7, 14 and 21 days using QIAzol (Qiagen, Hiden, Germany) and the RNeasy mini kit ...
13
Passage of Magnetic Tat Conjugated Fe3O4@SiO2 Nanoparticles Across In Vitro Blood Brain Barrier
... The effect of NPs across BBB was further evaluated by monitoring the changes of the TEER before and after the existence of NPs under the external magnetic field. As shown in Fig. 9, the TEER values exhibited a similar ...
12
Microglial cells in astroglial cultures: a cautionary note
... confluent monolayer, GFAP immunostaining results in an image in which virtu- ally the entire cultured area is covered by astrocytes, giving the false impression that there is not much room for other cells (Fig ...
11