Monolithic phases for bioanalysis using HPLC
Sample Preparation In Bioanalysis: A Review
... to HPLC or LCMS/MS Protein precipitation plates are compatible with small volume of solvent Protein precipitation plate contains hydrophobic PTFE membrane as a prefilter removes the unwanted precipitated proteins ...
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LC-MS in Drug Bioanalysis
... perform bioanalysis, which include immunoassays, gas chromatography- fl ame ionization detection (GC-FID), gas chromatography-mass spectrometry (GC-MS), high performance liquid chroma- tography-ultraviolet ...
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Development of novel methodologies for using ICP MS in bioanalysis and drug metabolism
... The HPLC-ICP-MS and HPLC-MSMS-derived intravenous plasma profiles as illustrated in Figure ...the HPLC-MSMS method, which followed the curve out to 3 hr when ...the HPLC-ICP-MS data were ...
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Metabolomics and bioanalysis of terpenoid derived secondary metabolites Muntendam, Remco
... cultivation using genetic analysis, HPLC-UV and 1 H-NMR based metabolome ...late phases of the growing processes, subsequently presenting important markers to follow up during the biosynthesis in ...
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Fluorescence Resonance Energy Transfer (FRET) Based Sensors for Bioanalysis
... encapsulation meets the above requirements [113-115]. Recently, liposomes have also been used to deliver proteins, antibiotics, anesthetics, oligonucleotides, antifungal and anticancer into cells and tissues ...
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Rapid determination of theaflavins by HPLC with a new monolithic column
... new HPLC method using a new monolithic column for the si- multaneous determination of four main theaflavin monomers in ...existing HPLC methods reported in the literature, and is less ...
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Ultralow-Temperature HPLC Using Low-Molecular-Weight Hydrocarbons as Mobile Phases
... ultralow-temperature HPLC, analyte retention is often enhanced, inhibiting ...a monolithic ODS ...mobile phases, and elution of mono- and di-chloromethane and n-octane, which were not eluted in our ...
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Assessment of Microstructure and Mechanical Behavior of Materials and Phases Observed in Low-Enriched Uranium Monolithic Fuel Plates After Fabrication
... analyzed using x-ray diffraction (XRD) with voltage and current of 45 keV and 40 mA, ...analyzed using HighScore Xpert Plus ...deconvoluted using the built-in peak identification and fitting ...
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Facile HPLC Separation of Peptides: Resolution of Chiral/Achiral Single Amino Acid Analogues using Teicoplanin-based Chiral Stationary Phases
... involves HPLC methods that are either complex or not ESI-MS ...the HPLC method development for resolution of small chiral peptides and single amino acid chiral/achiral analogues in peptide sequences ...
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Rapid micellar HPLC analysis of loratadine and its major metabolite desloratadine in nano-concentration range using monolithic column and fluorometric detection: application to pharmaceuticals and biological fluids
... constructed using spiked biological fluids as follows: 1 mL aliquots of human urine, plasma or breast milk samples were transferred into a series of 10-mL volumetric flasks, spiked with increas- ing concentrations ...
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HPLC Solvents and mobile phases
... for HPLC in order to satisfy the ever increasing demand requirements in terms of equipment and detection methods, particularly for impurities which, by interaction, can affect the result’s ...
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Studies of hexahelicene bonded phases for the HPLC resolution of enantiomers
... (TFAE) covalently bonded to silica.. 7 Interaction taking place on an -base Pirkle phase. This phase, which contains a 7r-base moiety, was designed to resolve solutes containin[r] ...
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HANDBOOK OF LC-MS BIOANALYSIS
... LC-MS Bioanalysis of Total Parent Drugs 471 Laixin Wang, Weiwei Yuan, Scott Reuschel, and Min Meng 38 LC-MS Bioanalysis of Reactive Compounds 479 Hermes Licea-Perez, Christopher ...
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HANDBOOK OF LC-MS BIOANALYSIS
... Cross-validations are needed under two scenarios: (1) when two or more unique methods are used in the same study and (2) two or more methods are used in a filing. In both cases, the intent is to demonstrate to the ...
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Monolithic SAW convolvers using chirp transducers
... Modified chirp transducers can be used to compensate for amplitude and phase errors produced elsewhere in the convolver.. A new design compensates for dispersion due to the waveguide,[r] ...
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BIOANALYSIS: AN EXTENSIVE APPLICATION IN PHARMACEUTICAL INDUSTRIES
... of bioanalysis in the pharmaceutical industry is to provide a quantitative measure of the active drug and/or its metabolite(s) for the purpose of pharmacokinetics, toxicokinetics, bioequivalence and ...
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OVERVIEW ON RECENT EXTRACTION TECHNIQUES IN BIOANALYSIS
... 3. Ion Exchange SPE Ion exchange SPE can be used for compounds that are charged when in a solution. Anionic compounds can be isolated on LC- SAX or LC-NH2 bonded silica cartridges. Cationic compounds are isolated by ...
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ASMS Regulated Bioanalysis Interest Group (RBIG) Workshop. Antibody-Drug Conjugates (ADC) A Complex Problem in Regulated Bioanalysis.
... Question period: What are the differences between an ADC and a monoclonal antibody (mAb)? For the ADC vs the mAb, you have to consider the small molecule conjugated payload attached to the mAb. For the unconjugated ...
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Label-free bioanalysis of Leishmania infantum using refractive index tomography with partially coherent illumination
... The study of Leishmania infection has attracted great attention from the scientific community because, according to the World Health Organization, 20000 people die annually from this disease [19] . To date, there is ...
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Supplier Selection Using Two Phases Model
... two phases, the first phase starts by the weighted mathematical model from an exciting example, and the second phase makes the check and refines the result from the first phase by checking the ...