mRNA Degradation
Characterising the role of murine gammaherpesvirus-68 host shutoff protein in mediating cellular mRNA degradation
... The discovery of host shutoff within the γ-herpesvirus family has led to the question of how the multi-functional DNA exonuclease could be causing mRNA degradation. The first and most obvious answer would ...
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SPT5 affects the rate of mRNA degradation and physically interacts with CCR4 but does not control mRNA deadenylation
... in mRNA metabolism, including that of transcriptional elongation, mRNA transport, and nu- clear exosome function, but the primary function of CCR4 and CAF1 is in the deadenylation and degra- dation of ...
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Selection and Characterization of RNA Interference-Deficient Trypanosomes Impaired in Target mRNA Degradation
... in degradation of target mRNA but accumulate wild-type levels of ...of degradation of tar- get mRNA upon electroporation of wild-type (Y2), RiD-1, and RiD-2 cells with increasing doses of each ...
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CRISPR mediated targeted mRNA degradation in the archaeon Sulfolobus solfataricus
... Our experiments confirm CRISPR-mediated RNA-cleavage as suggested earlier through in vitro results with purified CMR complexes in two different archaea (28,30). We dem- onstrate here in addition that the RNA interference ...
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mRNA Degradation Rates Are Coupled to Metabolic Status in Mycobacterium smegmatis
... increase mRNA degradation rates by increasing intracellular ATP ...Remarkably, mRNA half-lives were significantly decreased in response to INH ...bacterial mRNA being destabilized rather than ...
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Small Interfering RNAs That Deplete the Cellular Translation Factor eIF4H Impede mRNA Degradation by the Virion Host Shutoff Protein of Herpes Simplex Virus
... Vhs-mediated mRNA degradation following depletion of various cellular ...-actin mRNA to between 20 and 30% of those in mock-infected cells, indicative of a normal Vhs ...-actin mRNA levels ...
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mRNA degradation: an underestimated factor in steady state transcript levels of cytochrome c oxidase subunits?
... For many applications, the underlying cause of a change in transcript level is of less interest than the fact that the change has occurred. Conversely, transcript levels are often used to infer a change in gene ...
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Vaccinia virus induces cellular mRNA degradation.
... Purified viral core particles are capable of synthesizing early viral mRNAs Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest The infection of mouse L cells with vaccinia[r] ...
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In vitro mRNA degradation system to study the virion host shutoff function of herpes simplex virus.
... Finally, although the wild-type vhs function induced accelerated in vitro turnover of both viral and cellular mRNAs, endogenous 28S rRNA was equally stable in extracts from mock-infected[r] ...
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The Yeast Cytoplasmic LsmI/Pat1p Complex Protects mRNA 3′ Termini From Partial Degradation
... the mRNA is processed through ski4-1, and ski8⌬ mutations do not prevent trimming in the 5⬘ to 3⬘ mRNA degradation pathway and any 5⬘ the lsm1⌬ ...3⬘ degradation pathway. There was no 5⬘ end → ...
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The Effect of Herpes Simplex Virus Virion Host Shutoff Gene- a New Suicide Gene- on Tumor Cells
... initiates mRNA degradation near the 5′ end of RNA where the translation factors exist, although it cleaves mRNA at other locations through the molecule ...Vhs-dependent mRNA degradation ...
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Pgc suppresses the zygotically acting RNA decay pathway to protect germ plasm RNAs in the Drosophila embryo
... (pgc) mRNA is a component of the germ ...plasm mRNA degradation and pole cell death, suggesting that Pgc represses zygotic miRNA transcription in pole cells to protect germ plasm ...miRNA-mediated ...
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SYNTHESIS OF SPECIFIC, STABILIZED MESSENGER RNA WHEN TRANSLOCATION IS BLOCKED IN ESCHERICHIA COLI
... The notion of “coupl- ing” can be extended to the possible relationship of translation and mRNA degradation; for in uiuo, mRNA degradation has been shown to follow cl[r] ...
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A comparison of key aspects of gene regulation in Streptomyces coelicolor and Escherichia coli using nucleotide-resolution transcription maps produced in parallel by global and differential RNA sequencing
... understanding mRNA degradation, ...rpsT mRNA (panel A) and another downstream of the RNase III sites in adhE that has been mapped previously, but not analysed (Aristarkhov et ...of mRNA in ...
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Identification of ebs1, lsm6 and nup159 as suppressors of spt10 effects at ADH2 in Saccharomyces cerevisiae suggests post transcriptional defects affect mRNA synthesis
... To gain further insight into the mechanisms of how CCR4 activates gene expression, we have reconducted the screen for factors that can suppress spt10-enhanced expression at ADH2. The only known suppressors of spt10 ...
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Multiple Mechanisms for Degradation of Bacteriophage T4 soc mRNA
... of mRNA stability in a stage-dependent manner during ...rapid degradation of mRNAs. To investigate the mechanism of such mRNA degradation, we analyzed the late gene soc ...The ...
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Krzysztoń, Rafał Stanisław (2018): Towards efficient siRNA delivery and gene silencing kinetics on the single cell level. Dissertation, LMU München: Fakultät für Physik
... Yet, the major drawback of quantifying the siRNA silencing efficiency by comparative changes in reporter fluorescence intensities is the fact that the outcome is time dependent. Even when the CayRFP expression reference ...
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The Virion Host Shutoff Endonuclease (UL41) of Herpes Simplex Virus Interacts with the Cellular Cap-Binding Complex eIF4F
... vitro mRNA degradation system ...vivo mRNA degradation ...vivo mRNA degradative activities were determined in previous studies (4, 7, 8) and are summarized here for comparison to ...
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Mathematical modeling of signaling pathway dynamics and stochastic gene expression
... For example , Hoffmann et al. (Hoffmann et al., 2002) used an ODE based model to describe the temporal control of NF-κB, a transcriptional activator that regulates nu- merous genes involved in stress, injury, and immune ...
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Extensive exometabolome analysis reveals extended overflow metabolism in various microorganisms
... the central metabolism, e.g. membrane proteins, occurs to directly supply the necessary amino acids for protein synthesis (Figure 6C). In fact, this kind of starvation re- sponse was recently proposed for eukaryotic ...
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