mRNA export
Following Temperature Stress, Export of Heat Shock mRNA Occurs Efficiently in Cells with Mutations in Genes Normally Important for mRNA Export
... for export of heat shock ...and mRNA export ...lyzed export of SSA4 mRNA in the single-mutant strains each ...SSA4 mRNA and production of Ssa4p-GFP in yra1 ⌬ RRM (lacking aa 76 ...
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TREX exposes the RNA-binding domain of Nxf1 to enable mRNA export
... by mRNA, and it is not clear whether Nxf1 associates via protein-protein interactions with assembled TREX in ...rapid mRNA export block 8 though RNAi of most metazoan TREX components results in a ...
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Characterization of the Betaherpesviral pUL69 Protein Family Reveals Binding of the Cellular mRNA Export Factor UAP56 as a Prerequisite for Stimulation of Nuclear mRNA Export and for Efficient Viral Replication
... unspliced mRNA via recruitment of the cellular DExD/H-box RNA helicases UAP56 and URH49 (29), it was tempting to investigate whether this was also true for pC69 and ...CAT mRNA reporter assays clearly ...
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The Interaction of the Cellular Export Adaptor Protein Aly/REF with ICP27 Contributes to the Efficiency of Herpes Simplex Virus 1 mRNA Export
... viral mRNA export during HSV-1 ...viral mRNA export due to residual levels of Aly/REF and possible redundancy in mRNA adaptor function, as well as compensatory cellular regulation ...
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Transfer of the UAP56 Interaction Motif of Human Cytomegalovirus pUL69 to Its Murine Cytomegalovirus Homolog Converts the Protein into a Functional mRNA Export Factor That Can Substitute for pUL69 during Viral Infection
... To test this hypothesis, HEK293T cells were cotransfected with FLAG-UAP56 and myc-tagged versions of pUL69, pC69, pRh69, and pM69 (Fig. 1A). Two days later, cells were lysed and coimmu- noprecipitation (CoIP) was ...
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Perinuclear P granules are the principal sites of mRNA export in adult C elegans germ cells
... that mRNA export factors are highly enriched at the NPCs associated with P ...nascent mRNA traffics directly to P ...of mRNA in quiescent germ cells, presumably preventing translation of that ...
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An Interaction between KSHV ORF57 and UIF Provides mRNA-Adaptor Redundancy in Herpesvirus Intronless mRNA Export
... to export viral intronless mRNAs ...to export adaptor ...to export viral intronless mRNAs, as it is unable to bind to either Aly or UIF (Figure ...these export adaptors which lead Figure ...to ...
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Deficiency in the mRNA export mediator Gle1 impairs Schwann cell development in the zebrafish embryo
... 2 Abstract - GLE1 mutations cause lethal congenital contracture syndrome 1 (LCCS1), a severe autosomal recessive fetal motor neuron disease, and more recently have been associated with amyotrophic lateral sclerosis ...
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mRNA export correlates with activation of transcription in human subgroup C adenovirus-infected cells.
... late mRNA species from their cellular counterparts is not yet ...viral mRNA characteristic of the late phase of ...cellular mRNA species fail to enter the cytoplasm efficiently during the late phase ...
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Epstein-Barr Virus mRNA Export Factor EB2 Is Essential for Production of Infectious Virus
... viral genes involved in DNA replication could be detected by RT-PCR (Fig. 4B, lanes 2, 5, 8, 11, 14, and 17). This result was expected because under the conditions described above, oriLyt- dependent replication took ...
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mRNA export in the apicomplexan parasite Toxoplasma gondii: emerging divergent components of a crucial pathway
... Studies performed on trypanosomes also provide evi- dence of significant structural and functional differences in export factors with other eukaryotic lineages [13, 14]. Des- pite the high conservation of Sub2 in ...
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Epstein-Barr Virus mRNA Export Factor EB2 Is Essential for Intranuclear Capsid Assembly and Production of gp350
... an mRNA export ...virus mRNA export factor EB2 from the EBV genome and then introduced the mutated genome into 293 ...and export of early ...nuclear export of most, but not all, ...
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Regulation of mRNA Export by Nutritional Status in Fission Yeast
... the mRNA export defective rae1-167 mutation in Schizosaccharomyces ...and mRNA export defects of nup184 mutants when grown in nutrient-rich ...RNA export defects when nup184 mutant ...
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pUL69 of Human Cytomegalovirus Recruits the Cellular Protein Arginine Methyltransferase 6 via a Domain That Is Crucial for mRNA Export and Efficient Viral Replication
... viral mRNA export factor, facilitating the cytoplasmic accu- mulation of unspliced RNA via interaction with the cellular mRNA export factor ...and/or mRNA export ...efficient ...
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THOC5, a member of the mRNA export complex: a novel link between mRNA export machinery and signal transduction pathways in cell proliferation and differentiation
... of mRNA export machinery during differentiation has not been adequately ...and mRNA export are still ...total mRNA export in the steady state, however it influences more than 90% ...
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Dual Functions for the Schizosaccharomyces pombe Inositol Kinase Ipk1 in Nuclear mRNA Export and Polarized Cell Growth
... The mRNA export, temperature-sensitive growth, and morphological de- fects exhibited by ipk1 ⌬ cells are rescued by overexpressing the ipk1 ⫹ catalytic C-terminal domain but not the N-terminal do- ...
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THOC5/FMIP, an mRNA export TREX complex protein, is essential for hematopoietic primitive cell survival in vivo
... mature mRNA is then exported from the nucleus to the cytoplasm for ...Nuclear export of mRNA composes one part of a larger network of molecular events that begin with transcription of the mRNA ...
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Elucidating the Mechanism of mRNA Export Regulation by the Nuclear Pore Complex
... The DEAD-box family helicase DDX19 (Dbp5 in fungi) is an ATPase that binds mRNA and is responsible for the terminal catalytic step in mRNA export by the NPC. DEAD-box proteins are characterized by ...
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Mapping Interactions between mRNA Export Factors in Living Cells
... We have spatially mapped the site where TREX subunits associate with each other and the mRNA export receptor Nxf1. We found that the major sites for interaction between two TREX subunits and Nxf1 do not lie ...
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MEK ERK signaling controls Hdm2 oncoprotein expression by regulating hdm2 mRNA export to the cytoplasm
... p53 mRNA (61), this is unlikely to be the mechanism of p53 inactivation in our experiments, as U0126 does not prevent the accumulation of p53 protein in response to ...
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