mRNA splicing
Therapy-induced stress response is associated with downregulation of pre-mRNA splicing in cancer cells
... to mRNA splice variants ...alternative splicing events in treated cells ...alternative splicing variants was intron ...common splicing events and 39 of them involved in intron retention ...
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Evolution of NCoR 1 and NCoR 2 corepressor alternative mRNA splicing in placental mammals
... alternative mRNA splicing, generating an extensive series of corepressor protein variants that are expressed at different abun- dances in different tissues, preferentially associate with different ...
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Genetic Interactions With CLF1 Identify Additional Pre-mRNA Splicing Factors and a Link Between Activators of Yeast Vesicular Transport and Splicing
... pre-mRNA splicing as disruption of numerous lon and Shuman ...(cet1) splicing biological pathways ...also splicing impaired (Schwer and mutations in the genes encoding the NTC splicing ...
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Evidence That the pre-mRNA Splicing Factor Clf1p Plays a Role in DNA Replication in Saccharomyces cerevisiae
... Clf1p is an essential, highly conserved protein in S. cerevisiae that has been implicated in pre-mRNA splicing. Clf1p’s ortholog in Drosophila, Crn, is required for normal cell proliferation. Cells depleted ...
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spp42, Identified as a Classical Suppressor of prp4-73, Which Encodes a Kinase Involved in Pre-mRNA Splicing in Fission Yeast, Is a Homologue of the Splicing Factor Prp8p
... temperature. spp42 encodes a large protein (p275), which is the homologue of Prp8p. This protein has been shown in budding yeast and mammalian cells to be a bona fide pre-mRNA splicing factor. Taken ...
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Interpretation, Stratification and Validation of Sequence Variants Affecting mRNA Splicing in Complete Human Genome Sequences
... Human Splicing Pipeline software has been developed to analyze variants on a ...with splicing effects are displayed. Potential natural and cryptic mRNA splicing variants are identified, and ...
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Tip110 binding to U6 small nuclear RNA and its participation in pre-mRNA splicing
... pre-mRNA splicing. We then performed an in vitro splicing assay using a human β -globin minigene pre-mRNA as a ...vitro splicing assay [36, ...the splicing reaction and ...
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Development of an in vitro pre-mRNA splicing assay using plant nuclear extract
... vitro splicing system for plants has been long overdue; difficulties in developing one may be because of characteristics inherent to plant ...pre- mRNA splicing machinery is also ...vitro ...
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Overexpression of Essential Splicing Factor ASF/SF2 Blocks the Temporal Shift in Adenovirus Pre-mRNA Splicing and Reduces Virus Progeny Formation
... alternative splicing (reviewed in refer- ence ...distinct mRNA species accumulating at different time points of infection (reviewed in reference ...of splicing factors plays a critical role in this ...
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Analysis of autoregulation at the level of pre-mRNA splicing of the suppressor-of-white-apricot gene in Drosophila.
... Thus, S U ( W " ) protein provided in trans should repress SLe4 pre-mRNA splicing resulting in reduced fully spliced mRNA levels and concomitantly increased levels of i[r] ...
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A Splicing Enhancer in the E4 Coding Region of Human Papillomavirus Type 16 Is Required for Early mRNA Splicing and Polyadenylation as Well as Inhibition of Premature Late Gene Expression
... early mRNA production to some ...a splicing silencer in L1, which suggests that stronger regulation of L1 mRNA production occurs at the L1 3 ⬘ splice ...L1 mRNA splicing, most likely at ...
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An Rtf2 Domain-Containing Protein Influences Pre-mRNA Splicing and Is Essential for Embryonic Development in Arabidopsis thaliana
... the splicing machinery are not yet fully understood (Heyd and Lynch 2011; Reddy et ...alternative splicing, it is likely that additional proteins contributing to this process remain to be discovered (Chen ...
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Influenza Viruses and mRNA Splicing: Doing More with Less
... transcript splicing, it is also impor- tant to consider indirect regulation via the export of viral mRNAs, as the nucleocytoplasmic export machinery is closely interlinked with pre-mRNA splicing ...
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Hyperinsulinemia is associated with altered insulin receptor mRNA splicing in muscle of the spontaneously obese diabetic rhesus monkey
... development of insulin resistance and non-insulin-dependent diabetes mellitus (NIDDM), the spontaneously obese diabetic rhesus monkey, a unique model that is extraordinarily similar to human NIDDM, was used. ...
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Systematic Analyses of rpm-1 Suppressors Reveal Roles for ESS-2 in mRNA Splicing in Caenorhabditis elegans
... The fission yeast ortholog of ES2, Bis1, was found as an interacting protein to a stress-response nuclear envelope protein Ish1 (induced in stationary phase 1) and plays a role in viability in the stationary phase ...
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The Pre-mRNA Splicing Machinery of Trypanosomes: Complex or Simplified?
... trans splicing in ...trans splicing, the capped 5 ⴕ -terminal region of the small nuclear SL RNA is fused onto the 5 ⴕ end of each ...functional mRNA by providing the cap ...cis splicing ...
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Weight and nutrition affect pre mRNA splicing of a muscle gene associated with performance, energetics and life history
... Fig. 1. Variable N-terminal region of protein isoforms produced by alternative splicing of the Tnt gene in fall armyworm moths and Glanville fritillary butterflies. Alternative exons are colored. Traces on the ...
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Model Systems to Explore CPB2 Pre-mRNA Splicing
... residues from partially-degraded fibrin (41), many studies in the years following its discovery have uncovered evidence that TAFI also plays a role in the modulation of inflammation and the acute phase response. CPB2 ...
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Mutational analysis of pre-mRNA splicing in Saccharomyces cerevisiae using a sensitive new reporter gene, CUP1.
... A highly sensitive assay for 5' splice site usage was designed by engineering an ACTl-CUP1 construct with duplicated 5' splice sites; mutations were introduced into[r] ...
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Vex seq: high throughput identification of the impact of genetic variation on pre mRNA splicing efficiency
... In order to ensure that the variants are associated with the correct barcode, the 1˚ and 2˚ libraries were se- quenced using paired end amplicon sequencing (Fig. 2a). The results from sequencing the 1 ˚ library show that ...
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