MS/MS
BIOANALYTICAL METHOD DEVELOPMENT AND VALIDATION BY LC MS/MS: A REVIEW
... Bioanalytical methods employed for the quantitative determination of drugs and their metabolites in biological matrix (plasma, urine, saliva, serum etc.) play a significant role in evaluation and interpretation of ...
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Determination of lesinurad in rat plasma by a UHPLC–MS/MS assay
... Plasma samples were analyzed by the LC–MS/MS method. The system was composed of an Agilent 1290 LC system (Agilent Technologies, Santa Clara, CA, USA) with a 1.8 μm Rapid Resolution HT C18 column (3.0 × 100 ...
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Phytoestrogens in bovine plasma and milk – LC MS/MS analysis
... Chromatographic and detection conditions High performance liquid chromatography coupled to ion trap mass spectrometer was employed for the separation and detection of daidzein, genistein, and equol in bovine plasma and ...
11
NEWLY DEVELOPED AND VALIDATED METHOD FOR AZITHROMYCIN BY LC/MS-MS
... To the vials containing pipetted samples 3mL of organic mixture was added and vortexed. The samples were extracted for 10 min at 2000 rpm in vibromax and centrifuged for 10 min at 4000 rpm at 4°C. 2.4 mL of upper organic ...
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Stability indicating lc ms/ms method for determination of teicoplanin in human plasma
... Several analytical procedures areavailable in the literature for the estimation of Teicoplanin in biological fluids: fluorescence n immunoassays (FPIA), homogeneous turbidimetric immunoassays, HPLC and LC-MS. An ...
12
Exorphin Peptides In Urine With Hplc-Ms/Ms Detection
... Exorphins have been found in urine from individuals diagnosed with autism spectrum disorders by HPLC techniques. However, several studies, using sophisticated analytical techniques, have reported negative findings. This ...
7
Analysis of nine compounds from Alpinia oxyphyllafruit at different harvest time using UFLC-MS/MS and an extraction method optimized by orthogonal design
... The proposed UFLC-MS/MS method was subsequently applied to determine nine chemical markers including nootkatone (1), yakuchinone A (2), yakuchinone B (3), oxyphyllacinol (4), tectochrysin (5), izalpinin ...
9
A COMPARATIVE STUDY IN THE CONFIRMATORY METHOD DEVELOPMENT AND VALIDATION OF FLUBENDAZOLE IN FISH AND SHRIMP MUSCLES BETWEEN HPLC MS/MS (Q MICRO) AND UPLC MS/MS (TQD)
... 17. Viktor Cvilink, Lenka Skalova, Barbora Szotakova, Jiri Lamka, Risto Kostiainen, Raimo A Ketola, LC-MS/MS identification of Albendazole and Flubendazole metabolites formed in ex vivo by heamonchus ...
14
MS/MS studies for the selective detection of isomeric biogenic VOCs using a Townsend Discharge Triple Quadrupole Tandem MS and a PTR-Linear Ion Trap MS
... It is still a challenge to develop selective and sensitive schemes for quantitative and fast determination of isomeric BVOCs. Here we utilize a triple quadrupole tandem mass spectrometer (QqQ-MS) and the PTR-LIT, ...
10
Pharmacological potential of Bidens pilosa L and determination of bioactive compounds using UHPLC QqQLIT MS/MS and GC/MS
... Bioactive volatile compounds present in the B. pilosa meth- anolic leaves extract was analysed and identified using GC/ MS as described by Sen et al. [34] and Rufatto et al. [35] with some minor modifications. ...
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Isolation, purification and partial characterization of a glycoside from the Jamaican ball moss plant (Tillandsia recurvata L )
... Initial isolation and characterization screens have been carried out on the Jamaican ball moss (Tillandsia re- curvata L.) plant producing a novel complex glycosi- dic compound. This complex glycoside may be respon- ...
9
Biological and chemical analysis in the detection of methamidophos in soil: ecotoxicological test with liquid chromatography/mass spectrometry
... LC- MS/MS (0.19 µg L -1 ). Despite the exposed, the LOD of the LC-MS/MS was 59 ng L -1 , indicating that its LOQ can be reduced by adjusting in the parameters of the analytical .../ MS ...
7
The Schistosoma mansoni lipidome: Leads for immunomodulation
... collected MS/MS spectra according to each lipid class ...(tandem) MS spectra in the ESI þ mode, ensuring matching molecular masses with the FiehnO lipid data- base in MS-DIAL ...tandem ...
12
Ash leaf metabolomes reveal differences between trees tolerant and susceptible to ash dieback disease
... and MS/MS spectra were used to interrogate existing literature, and databases including, but not limited to, KNApSAcK ...used: MS/MS isolation window was set to first isotope only with default ...
14
Implementation and evaluation of amyloidosis subtyping by laser-capture microdissection and tandem mass spectrometry
... While the success rate for LCM-MS/MS amyloid iden- tification was high, interpretation is not always straight- forward and we note several caveats with this technique. Firstly, more than one amyloid forming ...
6
Mass spectrometric study of naproxen dimer anions generated from racemate and pure enantiomers
... The behaviour of naproxen dimer anions ([2Napr-H] - ) was studied by electrospray ionization mass spectrometry. Three different analyses were performed, namely direct inlet MS and HPLC/MS (performed for ...
8
Gaining insight into electron capture dissociation mass spectrometry of peptides and proteins
... Protein sequencing using mass spectrometry often incorporates enzymatic digestion prior to mass analysis to improve sequence coverage and identification. Commonly, the enzyme trypsin is used to cleave the protein as it ...
144
Detection and mechanistic comparison of two anti-trypanosomal diamidines in a rat renal model
... Figure 31 Lower limit of quantification standard (2.5 ng/mL) for furamidine in rat plasma analyzed by LC/MS/MS.. Figure 32 Untreated rat kidney analyzed by LC/MS/MS.[r] ...
173
Random and independent sampling of endogenous tryptic peptides from normal human EDTA plasma by liquid chromatography micro electrospray ionization and tandem mass spectrometry
... rich MS/MS spectra at a high signal-to-noise is the key to both reliable and sensi- tive identification and quantification of blood peptides and proteins with the X!TANDEM algorithm [23, ...the ...
15
Meier, Florian (2018): Data acquisition methods for next-generation mass spectrometry-based proteomics. Dissertation, LMU München: Fakultät für Chemie und Pharmazie
... N MS/MS scans 50,296 ...the MS/MS scans are selected in real-time (hence ‘data-dependent’) based on the survey ...high-quality MS/MS ...the MS level as well as with ...
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