Multiplex Immunoassay
Multiplex immunoassay characterization and species comparison of inflammation in acute and non-acute ischemic infarcts in human and mouse brain tissue
... A small sample size is not the only limitation of this study. Autolysis is a significant challenge for multiplex immunoassay investigations of human brain due to the difficulty of obtaining post-mortem ...
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Development and Standardization of a High-Throughput Multiplex Immunoassay for the Simultaneous Quantification of Specific Antibodies to Five Respiratory Syncytial Virus Proteins
... serological multiplex immunoassay (MIA) that determines and distinguishes antibodies against the five RSV glycoproteins postfusion F, prefusion F, Ga, Gb, and N ...
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Determination of Cytokine Protein Levels in Cervical Mucus Samples from Young Women by a Multiplex Immunoassay Method and Assessment of Correlates
... a multiplex immunoassay, recently showed significantly increased levels of IL-4, IL-10, and IL-13 in cervical secretions in gravid (first-tri- mester) smokers compared with gravid nonsmokers ...
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Development of an Extended-Specificity Multiplex Immunoassay for Detection of Streptococcus pneumoniae Serotype-Specific Antigen in Urine by Use of Human Monoclonal Antibodies
... enzyme immunoassay (EIA), multiplex immunoassay (MIA), single and multiplex PCRs, and capsular sequence typing (CST) (15–21), has contributed to serogroup/serotype surveillance of invasive and ...
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Sensitive and Specific Detection of Staphylococcal Epidermolysins A and B in Broth Cultures by Flow Cytometry Assisted Multiplex Immunoassay
... Two of the most common bacterial skin infections of young infants and children are bullous impetigo due to Staphylococcus aureus and its more acute form, staphylococcal scalded skin syndrome. Epidermolysin A (ETA), ETB ...
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Development and Evaluation of a Broad Bead Based Multiplex Immunoassay To Measure IgG Seroreactivity against Human Polyomaviruses
... In the HPyV multiplex immunoassay, serum samples (1:100) were incubated for 1 h in blocking buffer (1 mg/ml casein, 0.5% polyvinyl alcohol, 0.8% polyvinylpyrrolidone, 2.5% Super ChemiBlock [Chemicon ...
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2D-electrophoresis and multiplex immunoassay proteomic analysis of different body fluids and cellular components reveal known and novel markers for extended fasting
... extended fasting, revealed substantial between-subject variability which may mask the true biological effect of fasting on the proteome. This suggests that any nutrige- nomic experiment require a sufficient number of ...
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Fast, Antigen-Saving Multiplex Immunoassay To Determine Levels and Avidity of Mouse Serum Antibodies to Pertussis, Diphtheria, and Tetanus Antigens
... To enhance preclinical evaluation of serological immune responses to the individual diphtheria, tetanus, and pertussis (DTP) components of DTP combination vaccines, a fast hexavalent bead-based method was devel- oped. ...
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A multiplex immunoassay of serum biomarkers for the detection of uveal melanoma
... same multiplex immunoassay such as ...a multiplex immuno- ...the multiplex immunoassays is more analyte and sam- ple matrix dependent compared to monoplex immuno- assays ...
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A multiplex immunoassay for the non invasive detection of bladder cancer
... First, urinary concentrations of each of the ten bio- markers were normalized using urinary creatinine concentration. Next, we compared the diagnostic abil- ity associated with the combination of the individual ...
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Evaluation of a Validated Luminex-Based Multiplex Immunoassay for Measuring Immunoglobulin G Antibodies in Serum to Pneumococcal Capsular Polysaccharides
... ABSTRACT This article describes the results of a study designed to bridge the World Health Organization (WHO) pneumococcal enzyme-linked immunosorbent assay (ELISA) platform to the validated Luminex-based 13-plex direct ...
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Multiplex Immunoassay for Lyme Disease Using VlsE1-IgG and pepC10-IgM Antibodies: Improving Test Performance through Bioinformatics
... the multiplex assay and Western blotting for the 47 group 1 sera from patients with stage II/III disease (Table ...the multiplex assay was marginally more sensitive than Western blotting for ...
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Multiplex Immunoassay for Serological Diagnosis of Mycobacterium bovis Infection in Cattle
... chemiluminescence-based multiplex platform developed by Quansys Biosciences for use in nonveterinary assays has proven to be an ideal platform for the development of a multiplex assay for bTB (14, ...print ...
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Development of a Fluorescent-Bead-Based Multiplex Immunoassay To Determine Immunoglobulin G Subclass Responses to Neisseria meningitidis Serogroup A and C Polysaccharides
... fluorescent-particle-based multiplex flow cytometric immunoassay (MIA) for the detection of serum immunoglobulin G (IgG) and two IgG subclasses, IgG1 and IgG2, specific for Neisseria meningitidis serogroup ...
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Quantitative iTRAQ-Based Proteomic Identification of Candidate Biomarkers for Diabetic Nephropathy in Plasma of Type 1 Diabetic Patients
... the multiplex immuno-analysis appear quite ...the multiplex immunoas- say was conducted on untreated ...the multiplex immunoassay experiment, one patient had very high levels of apo A1, A2, C3 ...
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Feasibility of a Multiplex Flow Cytometric Bead Immunoassay for Detection of Anti-Epoetin Alfa Antibodies
... The clear need for the biotechnology industry to apply ro- bust, harmonized assays in this setting motivated our labora- tory to design a multiplex immunoassay platform. This ap- proach is gaining ...
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Neuroimmune expression in hip osteoarthritis: a systematic review
... Studies were grouped based on the technique used for neuroimmune expression measurements, namely: Beadbased multiplex immunoassay, Enzyme-Linked Immunosorbent Assay ELISA, quantitative r[r] ...
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Probiotic lactobacillus and estrogen effects on vaginal epithelial gene expression responses to Candida albicans
... A MultiBead W multiplex immunoassay (Enzo Life Sciences, Ann Arbor, MI) was used to quantify cytokines excreted into the basolateral growth media by VK2 cells. Using the manufacturer’s instructions, culture ...
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Identification of an age-dependent biomarker signature in children and adolescents with autism spectrum disorders
... Using multiplex immunoassay analysis of 208 mol- ecules we identified significantly different age-dependent trajectories in the levels of 12 proteins in ASD individ- uals compared to unaffected sibling ...
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Simultaneous Detection of Antibodies to Six Nonhuman-Primate Viruses by Multiplex Microbead Immunoassay
... Luminex-100 cytometer operation and data analysis. The Luminex-100 instru- ment was manufactured by Luminex Corp. and purchased from Upstate USA (Lake Placid, NY). The instrument was used at default settings, set by the ...
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