Multiplex reverse transcription-PCR
Rapid Identification of Nine Microorganisms Causing Acute Respiratory Tract Infections by Single Tube Multiplex Reverse Transcription PCR: Feasibility Study
... A multiplex reverse transcription-PCR (RT-PCR) assay was developed to allow in one test the detection of nine different microorganisms (enterovirus, influenza A and B viruses, ...
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Multiplex Reverse Transcription PCR for Simultaneous Surveillance of Influenza A and B Viruses
... (FluA/B) multiplex reverse transcription-PCR (RT- PCR) method that amplifies the most critical genomic segments (hemagglutinin [HA], neuraminidase [NA], and matrix [M]) of seasonal ...
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Multiplex reverse transcription PCR for surveillance of influenza A and B viruses in England and Wales in 1995 and 1996
... (multiplex) reverse transcription-PCR (RT-PCR) for detection, typing, and subtyping of the hemagglutinin gene of influenza type A (H3N2 and H1N1) and type B viruses was developed and ...
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Combining Multiplex Reverse Transcription PCR and a Diagnostic Microarray To Detect and Differentiate Enterovirus 71 and Coxsackievirus A16
... with multiplex reverse transcription-PCR to try to develop a method of sensitively and accurately detecting and differentiating EV71 and ...real-time PCR and subsequently confirmed by ...
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Comparison between Saliva and Nasopharyngeal Swab Specimens for Detection of Respiratory Viruses by Multiplex Reverse Transcription PCR
... Patients and specimens. We conducted a prospective study using paired NPS and saliva specimens from adult male patients, within 1 week after respiratory symptom onset, at the Armed Forces Medical School (Daejeon, ...
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Rapid Virological Diagnosis of Central Nervous System Infections by Use of a Multiplex Reverse Transcription PCR DNA Microarray
... The second phase of the evaluation consisted of the retro- spective analysis of 78 CSF samples initially tested negative or positive for either herpesviruses or HEVs by standardized commercially available RT-PCR ...
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Heminested Multiplex Reverse Transcription PCR for Detection and Differentiation of Norwalk Like Virus Genogroups 1 and 2 in Fecal Samples
... heminested multiplex PCR that would produce amplicons of different molecular weights for each of the NLV geno- ...the multiplex PCR also include the 63-nucleotide (nt) re- gion between the ...
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Simultaneously Typing Nine Serotypes of Enteroviruses Associated with Hand, Foot, and Mouth Disease by a GeXP Analyzer Based Multiplex Reverse Transcription PCR Assay
... GeXP multiplex assay consisted of 11 pairs of chimeric primers (including one pair of panenterovirus primers and 10 pairs of human enterovirus serotype-specific primers) and one pair of universal primers ...
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Detection of Human Picornaviruses by Multiplex Reverse Transcription PCR and Liquid Hybridization
... qualitative multiplex reverse transcription (RT)-PCR and liquid hybridization assay for the detection of human enteroviruses, rhinoviruses, parechoviruses, and Aichi virus was ...the ...
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Identification of New Influenza B Virus Variants by Multiplex Reverse Transcription PCR and the Heteroduplex Mobility Assay
... Epidemics caused by influenza viruses have been a major public health challenge. Although not responsible for pandem- ics, influenza B virus infections contribute substantially to the disease burden each year and account ...
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Development of Multiplex PCR Ligase Detection Reaction Assay for Detection of West Nile Virus
... novel multiplex reverse transcription-PCR ligase detection reaction (RT-PCR/LDR) assay for the detection of West Nile virus (WNV) in both clinical and mosquito pool ...the PCR is ...
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Reverse transcription multiplex PCR for differentiation between polio and enteroviruses from clinical and environmental samples
... to reverse transcription multiplex PCR with a primer pair which detects all enteroviruses ...The PCR products can unequivocally be identified by their lengths in agarose gels, whereas ...
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Multiplex real-time reverse transcription polymerase chain reaction for diff erential detection of H5, N1, and N8 genes of highly pathogenic avian infl uenza viruses
... silico PCR software application ...for PCR, and will indicate the likelihood of PCR success with each primer set against given target sequences (Kalendar et ...
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Multiplex Real Time Reverse Transcription PCR Assay for Determination of Hepatitis C Virus Genotypes
... A variety of methods have been used to determine hepatitis C virus (HCV) genotypes. Because therapeutic decisions for chronic HCV-related hepatitis are made on the basis of genotype, it is important that genotype be ...
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Preparation of Armored RNA as a Control for Multiplex Real Time Reverse Transcription PCR Detection of Influenza Virus and Severe Acute Respiratory Syndrome Coronavirus
... Primers and probes. Primers and probes were designed to amplify highly con- served regions of the matrix protein (M) gene of influenza A virus (Am fragment) and the M gene of influenza B virus (Bm fragment). Primers and ...
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Comparison of Viral Isolation and Multiplex Real Time Reverse Transcription PCR for Confirmation of Respiratory Syncytial Virus and Influenza Virus Detection by Antigen Immunoassays
... ences in the quality of specimens between age groups are plausible explanations (41). This would help explain the low Directigen A ⫹ B assay sensitivity reported here, since de- spite the testing of samples from patients ...
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DETECTION OF VIABLE SALMONELLA TYPHI USING THREE PRIMER PAIRS SPESCIFIC TO INVA, IVAB, AND FLIC-D GENES
... antigen (or H1-d antigen), the prt gene (previously known as rfbS), the gene encoding paratose synthase, the tyv gene (previously known as rfbE), the gene encoding CDP- tyvelose epimerase, and the ViaB region, a ...
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Multiplex, DNase free one step reverse transcription PCR for Plasmodium 18S rRNA and spliced gametocyte specific mRNAs
... Plasmodium gametocytes can be detected with mRNA- based molecular methods at sub-microscopic densities. Commonly targeted gametocyte-specific P. falciparum mRNAs include those made from single exon genes pfs25 and pfs230 ...
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Multiplex reverse transcriptase pcr assay for the detection of infective stage wuchereria bancrofti and its vector culex quinquefasciatus
... by Reverse Transcription reaction and amplification by PCR assay for determining the sensitivity of the optimum pool size in the detection of infective ...The Reverse transcription ...
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Multiplex PCR for Typing and Subtyping Influenza and Respiratory Syncytial Viruses
... A multiplex reverse transcription (RT)-PCR method that has been developed is capable of detecting and subtyping influenza A (H1N1 and H3N2) and B viruses as well as respiratory syncytial virus ...
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