Multiplex TaqMan real-time PCR
Multiplex real-time PCR assay for detection and differentiation of Bordetella pertussis and Bordetella parapertussis.
... Real-time PCR. Multiplex TaqMan real-time PCR was performed in the format of two duplex reactions using ABI 7500 system (Applied Biosystems) in a volume of 25 µl in ...
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Rapid Multiplex PCR and Real Time TaqMan PCR Assays for Detection of Salmonella enterica and the Highly Virulent Serovars Choleraesuis and Paratyphi C
... Salmonella-specific PCR band; the 461-bp and 709-bp bands for serovar Choleraesuis; and the 461-bp, 709-bp, and 986-bp PCR bands specific for serovar Paratyphi C were pro- duced ...
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Multiplex, Quantitative, Real Time PCR Assay for Cytomegalovirus and Human DNA
... resources. TaqMan Universal PCR master mix (2⫻), containing AmpliTaq Gold DNA polymerase, deoxynucleoside triphos- phates with dUTP, AmpErase UNG, Passive Reference 1, and optimized buffers, was purchased ...
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Multiplex Real Time PCR for Detection of Anaplasma phagocytophilum and Borrelia burgdorferi
... the TaqMan assay was tested with DNA extracted from the follow- ing organisms: Rickettsia rickettsii Sheila Smith strain, Anaplasma marginale Flor- ida strain, Rickettsia prowazekii Brienl strain, Escherichia coli ...
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Use of Multiplex Real Time PCR To Diagnose Scrub Typhus
... of PCR and serologic tests as diagnostic ...a multiplex real-time PCR assay using hydrolysis (TaqMan) probes targeting ...this PCR assay, we se- lected patients with ...
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Comparison of a Multiplex Real Time PCR Assay with a Multiplex Luminex Assay for Influenza Virus Detection
... virus real-time RT-PCR. The influ- enza virus RT-PCR TaqMan assay detects six targets: FluA M, FluB M, H3, H1, 2009 H1, and ...laboratory-developed real-time RT-PCR ...
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Development and evaluation of a one step multiplex real time TaqMan® RT qPCR assay for the detection and genotyping of equine G3 and G14 rotaviruses in fecal samples
... a PCR activation step at 95 °C for 5 min; 40 cycles of denaturation (95 °C for 15 s), an- nealing (47 °C for 15 s) and extension (68 °C for 2 min); and a final extension at 68 °C for 5 ...min. PCR products ...
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Multiplex Real Time PCR shortTUB Assay for Detection of the Mycobacterium tuberculosis Complex in Smear Negative Clinical Samples with Low Mycobacterial Loads
... was then conducted with DNA extracted from the clinical samples. The total reaction volume (20 l) included 10 l of LightCycler 480 DNA Probes Master, 5 l of DNA sample, 0.75 l of each primer (concentration 100 ...
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Evaluation of a Multiplex Real Time Reverse Transcriptase PCR Assay for Detection and Differentiation of Influenza Viruses A and B during the 2001 2002 Influenza Season in Israel
... The multiplex real-time TaqMan assay for influenza virus B was not as good (sensitivity, ...The multiplex TaqMan assay missed four influenza virus B-positive samples that were ...
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Novel Method for Genotyping Hepatitis B Virus on the Basis of TaqMan Real Time PCR
... on TaqMan real-time PCR, which identifies all HBV genotypes without post-PCR ...four multiplex real-time PCRs, each targeting two or three genotype-specific ...
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A TaqMan real time PCR assay for the detection and quantitation of Plasmodium knowlesi
... published real-time PCR assay by Rougemount et al that screens for all human species of Plasmodium and identifies the species using specific probes ...The real-time assay by Rouge- ...
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Rapid Detection of bla KPC Carbapenemase Genes by Internally Controlled Real Time PCR Assay Using Bactec Blood Culture Bottles
... Here we report on the development and validation of a TaqMan multiplex qPCR assay for the rapid detection of bla KPC genes from Bactec bottles. The assay, which includes human gene RNase P as an internal ...
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Comparative study of bacteriological culture and real-time fluorescence quantitative PCR (RT-PCR) and multiplex PCR-based reverse line blot (mPCR/RLB) hybridization assay in the diagnosis of bacterial neonatal meningitis
... Molecular methods, including RT-PCR and mPCR/ RLB, do not depend on the presence of viable or grow- ing bacteria, and thus are suitable to the detection of pathogens that cannot be cultured readily by routine ...
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Recovery of Cryptosporidium from spiked water and stool samples measured by PCR and real time PCR
... nested real time ...a TaqMan probe, CPrP3-1, were designed by Minarovicova et ...Each PCR mixture (total volume 10 µl) contained 8pM of each outer primer and the probe (Genomed, Poland), ...× ...
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Comparison of Anyplex II RV16 with the xTAG Respiratory Viral Panel and Seeplex RV15 for Detection of Respiratory Viruses
... plex PCR and sequencing. The primers for monoplex PCR in the single or nested PCR format were identical with the primers of the RV15 or RV16 ...The PCR products were purified with a power gel ...
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Evaluation of Real Time PCR versus PCR with Liquid Phase Hybridization for Detection of Enterovirus RNA in Cerebrospinal Fluid
... two-step real-time PCR methods for the quantification of enterovirus in CSF, one for the ABI 7700 TaqMan platform and the other for the Roche LightCycler ...two-step real-time ...
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A Comparison of the Clinical and Molecular Diagnosis of Herpes Simplex Keratitis
... of PCR negative results for HSV1 in samples clinically di- agnosed as typical ...by real time PCR to achieve a concentration similar to that found in HSV1 PCR positive clinical samples ...
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A TaqMan real time PCR assay for Rhizoctonia cerealis and its use in wheat and soil
... Amount of Rhizoctonia cerealis DNA detected pg/g soil in 92 UK field soils under different crops at two time points spring and autumn Previous crop.. Spring Allium Oilseed Rape Pasture P[r] ...
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Revisiting Pneumococcal Carriage by Use of Broth Enrichment and PCR Techniques for Enhanced Detection of Carriage and Serotypes
... The use of broth enrichment appeared to play a significant role in enhancing the identification of pneumococcus-positive specimens and detecting pneumococcal serotypes by both the culture- and PCR-based ...
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Detection and Genotyping of Varicella Zoster Virus by TaqMan Allelic Discrimination Real Time PCR
... The TaqMan assay and two PCR-RFLP methods were used to genotype 125 Canadian strains, 8 Australian strains, the P-Oka strain, and the V-Oka ...the TaqMan method corresponded with both RFLP methods ...
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