myc-tagged
Stress-Inducible Alternative Translation Initiation of Human Cytomegalovirus Latency Protein pUL138
... individual myc-tagged ORFs amplified from pEF1/myc-His-B expression plas- mids containing each myc-tagged ORF using the EcoRV restriction ...
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Maintenance of Endoplasmic Reticulum (ER) Homeostasis in Herpes Simplex Virus Type 1-Infected Cells through the Association of a Viral Glycoprotein with PERK, a Cellular ER Stress Sensor
... cells, myc-tagged polypeptides of the expected size were recovered following transfection of plasmids expressing the WT, ⌬ KD, or KA PERK derivative ...WT myc-tagged protein is activated ...
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Proteomic Analysis of Phagocytosis in the Enteric Protozoan Parasite Entamoeba histolytica
... We confirmed the phagosome localization of representative phagosome proteins Hgl, Igl, CP1, DPAP, Rab7A, and Rap2 by immunofluorescence assay as previously described (30) (Fig. 1). Trophozoites were incubated with ...
5
Modulation of Mitogen-Activated Protein Kinase-Activated Protein Kinase 3 by Hepatitis C Virus Core Protein
... with Myc-tagged core and Flag-tagged MAPKAPK3/MAPKAPK2 were immunoprecipitated with an anti-Myc antibody and bound protein was immunoblotted with an anti-Flag ...
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Studies of DJ-1, Parkin and Alpha-Synuclein Give Insights into Plausible Mechanisms for Parkinson's Disease Pathogenesis.
... has been identified as one of the putative substrates for parkin (72;257). Several of the pathogenic mutations in parkin, including the T240R mutation, have been shown to impair the ability for parkin to promote the ...
245
Chemical chaperone 4-phenylbutyrate prevents endoplasmic reticulum stress induced by T17M rhodopsin
... Proteins that are terminally misfolded in the ER may be degraded by the proteasome via ERAD. Typically, such proteins are unstable and show a reduced half-life. Be- cause ERAD substrates are typically ubiquitinated [14], ...
8
FBW7 regulates DNA interstrand cross-link repair by modulating FAAP20 degradation
... inhibition, myc- tagged FAAP20 was able to co-immunoprecipitate HA- tagged FBW7 whereas the FAAP20 SA mutant failed to do so, suggesting that FBW7 recognizes the phosphorylated CPD motif and ...
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Proteins that interact with the sodium-dependent glutamate transporters
... B) COS-7 cells were transfected with full-length myc-tagged ajuba alone. The distribution of the protein was detected by immunofluorescence using confocal microscopy.[r] ...
311
Intracellular Localization Map of Human Herpesvirus 8 Proteins
... the Myc tag, a GFP- tagged K13 protein (K13-GFP) was expressed in HeLa cells and detected by direct fluorescence analysis ...a Myc and a GFP tag ...both Myc-tagged ...the Myc tag ...
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Invasion of Host Cells by JC Virus Identifies a Novel Role for Caveolae in Endosomal Sorting of Noncaveolar Ligands
... Confocal microscopy/constructs. Green fluorescent protein (GFP)-Rab constructs GFP-Rab5, GFP-Rab7, GFP-Rab11, GFP-Rab5-S34N, GFP-Rab7- T22N, and GFP-Rab11-S25N were kind gifts of Stephen Furguson (Robart Research ...
12
HIV 1 integrase modulates the interaction of the HIV 1 cellular cofactor LEDGF/p75 with chromatin
... FLAG- tagged LEDGF/p75 was detected with anti-FLAG Mab (1/500, M2, Sigma) while non-tagged LEDGF/p75 was detected with an anti-LEDGF/p75 (1/500, clone 26/ LEDGF, BD Biosciences), Myc-tagged ...
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HIV 1 protease cleaves the serine threonine kinases RIPK1 and RIPK2
... Having established that ectopically expressed or puri- fied HIV-1 PR can cleave RIPK1 and RIPK2 in various cell types, we next asked whether RIPK1 and RIPK2 are cleaved during HIV-1 infection. First, we over-expressed ...
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Detection of the HIV-1 Minus-Strand-Encoded Antisense Protein and Its Association with Autophagy
... mutant in the context of the full-length untagged proviral DNA af- fected HIV-1 replication. ASP-mutated pNL4.3ASP67stop was first compared to parental wild-type pNL4.3 in transfected 293T and HeLaCD4-LTR/  gal cells ...
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Blockade of Human Immunodeficiency Virus Type 1 Expression by Caveolin-1
... Cav-2 also inhibits HIV virion production. Cav-1 is one of three known members of the caveolin gene family. Cav-1 and Cav-2 are widely coexpressed in different cell types and form hetero-oligomers, while Cav-3 is ...
13
Characterisation of the mouse glycosyl phosphatidylinositol-phospholipase D (GPI-PLD) gene
... Figure 4.6 Expression of Myc-tagged GPI-PLD proteins in transient transfected 293T cells.... 155.[r] ...
264
Epstein-Barr Virus Nuclear Antigen 3C Augments Mdm2-Mediated p53 Ubiquitination and Degradation by Deubiquitinating Mdm2
... either Myc-tagged full-length EBNA3C or different EBNA3C truncated mutants as indi- cated ...were tagged with a Myc ...different Myc-tagged EBNA3C constructs expressing either ...
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Nonstructural Protein 5A Impairs DNA Damage Repair: Implications for Hepatitis C Virus-Mediated Hepatocarcinogenesis
... interaction. For this purpose, HEK293T cells were cotransfected with Flag-tagged RAD51AP1, V5-tagged UAF1, and Myc-tagged NS5A. At 48 h posttransfection, cell lysates were immunoprecipitated ...
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SIAH-1 Interacts with the Kaposi's Sarcoma-Associated Herpesvirus-Encoded ORF45 Protein and Promotes Its Ubiquitylation and Proteasomal Degradation
... containing myc-tagged wild-type SIAH-1 and SIAH-1 mutated at the RING finger (C55A/C59H/C72S) were obtained from Simone Engelender (Technion, Israel) ...
11
MTCBP-1 Protein Expression is Enhanced in Fat Overloaded HepG2
... Lipid overloaded and transfected HepG2 grown in coverslips were washed three times in PBS and fixed with 4% paraformaldehyde in PBS. The cells were then permeabilised in 0.3% Triton X-100 in PBS for 10 minutes and ...
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Tumor suppressor Spred2 interaction with LC3 promotes autophagosome maturation and induces autophagy-dependent cell death
... Myc-tagged Spred2 and deletion mutants (ΔEVH1, ΔKBD and ΔSPR) were kindly provided by Prof. John K Heath. Mutations for tryptophan 370/leucine 373 and tyrosine 386/leucine 389 were mutated to Alanine in ...
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