Mycobacterial isolates
Detection and Differentiation of Mycobacterium tuberculosis and Nontuberculous Mycobacterial Isolates by Real Time PCR
... Mycobacteria cause a variety of illnesses that differ in severity and public health implications. The differ- entiation of Mycobacterium tuberculosis from nontuberculous mycobacteria (NTM) is of primary importance for ...
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Clinical, molecular and drug sensitivity pattern of mycobacterial isolates from extra pulmonary tuberculosis cases in Addis Ababa, Ethiopia
... Therefore, in this study we tried to evaluate the genetic diversity, clinical manifestation and drug sensitivity of mycobacterial isolates causing extra pulmonary tuberculosis is Addis A[r] ...
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Characterization of mycobacterial isolates phylogenetically related to, but different from Mycobacterium simiae
... the isolates appeared to be phylogenetically related to ...Six isolates repre- senting the largest of groups defined by means of genetic sequencing turned out to belong to the newly described species ...
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Biochip System for Rapid and Accurate Identification of Mycobacterial Species from Isolates and Sputum
... of mycobacterial species from isolates and clinical samples is important for patho- genic diagnosis and treatment and for disease ...common mycobacterial species by targeting the differences in the ...
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Comparison of Three Methods for Rapid Identification of Mycobacterial Clinical Isolates to the Species Level
... triphosphates (dTTP, dATP, dCTP, and dGTP) (Saibaisheng, Beijing, China), 0.4 M (each) primer, 10 to 100 g of genomic DNA, and 1 unit of Taq polymerase (Saibaisheng, Beijing, China). Special primers (forward primer, ...
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Identification of mycobacteria by high performance liquid chromatography
... Previously, we reported high-performance liquid chromatography HPLC patterns of the mycolic acids of seven species of mycobacteria that make up 90% of mycobacterial isolates in clinical [r] ...
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Antimicrobial susceptibility testing of rapidly growing mycobacteria isolated in Japan
... growing mycobacterial strains, re- gardless of species, showed good sensitivity to tigecyc- ...all isolates was inhibited by tigecycline at very low MICs, that is, ...ing mycobacterial ...
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<p>Epidemiology Of Human Pulmonary Infection With Nontuberculous Mycobacteria In Southeast China: A Prospective Surveillance Study</p>
... all mycobacterial isolates in ...NTM isolates from whole specimens was ...NTM isolates among the latter ...NTM isolates, 23 which may serve as an important explanation for the increased ...
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Application of the Sherlock Mycobacteria Identification System Using High Performance Liquid Chromatography in a Clinical Laboratory
... each mycobacterial isolate, internal standard, and con- trol were substantially less than those for testing isolates and controls using either traditional biochemical tests or nucleic acid ...the ...
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Direct Detection and Identification of Mycobacterium tuberculosis and Mycobacterium bovis in Bovine Samples by a Novel Nested PCR Assay: Correlation with Conventional Techniques
... M. bovis and M. tuberculosis strains from different geographical regions (32). The limit of detection by the N-PCR assay was shown to be five bacilli, equivalent to 50 fg of DNA. Kidane et al. (23) and Shah et al. (37) ...
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Cultivation of Nocardia spp on chemically defined media for selective recovery of isolates from clinical specimens
... We evaluated 44 Nocardia isolates, 18 group IV mycobacterial isolates, and 4 Streptomyces isolates for growth on blood agar BA and on carbon-free agar containing single or double substra[r] ...
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Tuberculosis Outbreak in an Educational Institution in Norway
... these isolates were identical at the genome level, they were likely part of a single transmission chain, and that transmission occurred in ...many isolates or by the presence of heterozygous calls in all or ...
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Characterization of Mycobacterium caprae Isolates from Europe by Mycobacterial Interspersed Repetitive Unit Genotyping
... caprae isolates by mycobacterial interspersed repetitive unit (MIRU) genotyping and by ...The isolates originated from 128 distinct epidemiological settings in 10 countries, spanning a period of 25 ...
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Species Specific DNA Profiling Mycobacterial Genomes Using Polymerase Chain Reaction with Single Universal Primer (UP-PCR)
... tuberculosis isolates from different geographical regions gained coinciding characteris- tic bands even though variation in minor bands could be seen ...tuberculosis isolates, one of them developed ...
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Polymorphism of Variable Number Tandem Repeats at Multiple Loci in Mycobacterium tuberculosis
... the isolates containing the deletion in the allele of VNTR4052 was relatively low and had no correlation with IS6110 patterns, suggesting that deletion in this region ...the isolates containing a partial ...
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Genetic characterization of Mycobacterium tuberculosis in the West Bank, Palestinian Territories
... The study of theTbD1 region showed that the major- ity of isolates (n = 28) belonged to the TbD1 deleted modern strains, whereas only 3 strains (two M. bovis and one EAI strain) had TbD1 intact. However, all EAI ...
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Nineteen Cases of Buruli Ulcer Diagnosed in Japan from 1980 to 2010
... It is noteworthy that M. ulcerans subsp. shinshuense was identified in all of the isolates from Japanese patients diag- nosed with BU. M. ulcerans subsp. shinshuense, not M. ulcerans, could be the primary ...
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Pulmonary Mycobacterium avium intracellulare is the main driver of the rise in non tuberculous mycobacteria incidence in England, Wales and Northern Ireland, 2007–2012
... The continuing rise in the isolation of NTM between 2007 and 2012, driven primarily by pulmonary MAC in older individuals warrants further investigation. It is now imperative that standardised clinical data are collected ...
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Molecular Typing of Mycobacterium tuberculosis by Mycobacterial Interspersed Repetitive Unit Variable Number Tandem Repeat Analysis, a More Accurate Method for Identifying Epidemiological Links between Patients with Tuberculosis
... In contrast to IS6110-PGRS-based RFLP typing, PCR-based MIRU-VNTR typing or spoligotyping can be applied with crude DNA extracts from heat-killed mycobacterial cultures as soon as they become positive, avoiding ...
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Accelerated Detection and Identification of Mycobacteria with MGIT 960 and COBAS AMPLICOR Systems
... The MGIT system was evaluated in the clinical laboratory routine with samples from a population in whom M. tubercu- losis and MOTT are equally common as clinical isolates. It was compared to cultivation by using ...
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