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Myogenic cells

Determination, diversification and multipotency of mammalian myogenic cells

Determination, diversification and multipotency of mammalian myogenic cells

... dividing myogenic cells (to cope with the growing size of the embryo) and at the same time to generate differentiated, post-mitotic skeletal muscle fibers (to allow early movements of the ...of cells ...

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The p75NTR mediated effect of nerve growth factor in L6C5 myogenic cells

The p75NTR mediated effect of nerve growth factor in L6C5 myogenic cells

... All experiments were carried out on L6C5 rat myogenic cells (ICLC, AL00001). As already described [16], L6C5 myoblast cultures were maintained and subcultured in growing medium (GM) (DMEM with 4.5 g/l ...

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Determination, diversification and multipotency of mammalian myogenic cells

Determination, diversification and multipotency of mammalian myogenic cells

... dividing myogenic cells (to cope with the growing size of the embryo) and at the same time to generate differentiated, post-mitotic skeletal muscle fibers (to allow early movements of the ...of cells ...

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RhoA and ERK signalling regulate the expression of the transcription factor Nfix in myogenic cells

RhoA and ERK signalling regulate the expression of the transcription factor Nfix in myogenic cells

... deriving myogenic cells from pluripotent stem cells (Chal et ...robust myogenic differentiation are ...differentiated myogenic cells, through manipulation of RhoA/ROCK signalling ...

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Satellite Cell Plasticity in the Chicken Embryo

Satellite Cell Plasticity in the Chicken Embryo

... Satellite cells are defined by their morphological criteria as occupying grooves between the basal lamina and sarcolemma of the muscle ...Satellite cells define a unique lineage of myogenic ...

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Repairing skeletal muscle: regenerative potential of skeletal muscle stem cells

Repairing skeletal muscle: regenerative potential of skeletal muscle stem cells

... satellite cells, myogenic progenitors localized between the basal lamina and the muscle fiber ...have myogenic potency. Here, we discuss the main properties of satellite cells and other ...

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The emerging role of viral vectors as vehicles for DMD gene editing

The emerging role of viral vectors as vehicles for DMD gene editing

... progenitor cells and non-muscle cells with myogenic ...human myogenic cells harboring the various DMD mutations, which are not represented in the currently available animal ...patient ...

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Myogenic stem cells for the therapy of primary myopathies: wishful thinking or therapeutic perspective?

Myogenic stem cells for the therapy of primary myopathies: wishful thinking or therapeutic perspective?

... stem cells play a real role in adult tissue homeostasis, or are they just a remnant of embry- onic and fetal life that is rapidly lost after birth? Defini- tive hematopoietic stem cells derive from an ...

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Establishment of clonal myogenic cell lines from severely affected dystrophic muscles - CDK4 maintains the myogenic population

Establishment of clonal myogenic cell lines from severely affected dystrophic muscles - CDK4 maintains the myogenic population

... muscle-derived myogenic cells, we used the same strategy as previously described for normal skeletal-muscle cells; that is, ectopic expression of human telomerase reverse tran- scriptase (hTERT) to ...

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Matrix scaffolding for stem cell guidance toward skeletal muscle tissue engineering

Matrix scaffolding for stem cell guidance toward skeletal muscle tissue engineering

... of cells and DNA content from native ECM, car- ried out by employing physicochemical agents, enzymes, detergents, or even a combination of these ...of cells, showing comparable biomechanical features with ...

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Cell autonomous Notch activity maintains the temporal specification potential of skeletal muscle stem cells

Cell autonomous Notch activity maintains the temporal specification potential of skeletal muscle stem cells

... stem cells produces temporally distinct progeny until development is ...differentiated cells are generated during ...committed cells are required for maintaining muscle stem ...of myogenic ...

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Review: Skeletal muscle development in vertebrate animals

Review: Skeletal muscle development in vertebrate animals

... Abstract: This review covers the pre- and post-natal development of skeletal muscle of vertebrate animals with cellular and molecular levels. The formation of skeletal muscle initiates from paraxial mesoderm during ...

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Connective tissue cells, but not muscle cells, are involved in establishing the proximo distal outcome of limb regeneration in the axolotl

Connective tissue cells, but not muscle cells, are involved in establishing the proximo distal outcome of limb regeneration in the axolotl

... mesoderm-derived cells can form recognisable limb segments in the absence of muscle formation and it has been proposed that they are a dominant cell type directing limb patterning (Christ et ...including ...

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A homeo paired domain binding motif directs Myf5 expression in progenitor cells of limb muscle

A homeo paired domain binding motif directs Myf5 expression in progenitor cells of limb muscle

... progenitor cells in limbs by direct interaction with Pax3 (Bajard et ...its myogenic derivatives (Borycki et ...Moreover, cells overexpressing Pax3 were shown to activate Myf5 expression, although ...

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MYOD mediates skeletal myogenic differentiation of human amniotic fluid stem cells and regeneration of muscle injury

MYOD mediates skeletal myogenic differentiation of human amniotic fluid stem cells and regeneration of muscle injury

... hAFS cells were detached physically from culture dishes using a cell scrapper and sonicated in RIPA buffer (50 mM Tris–HCl pH 8.0, 150 mM NaCl, 1% NP-40, 0.5% sodium deoxycholate, 0.1% sodium dodecyl sulfate ...

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Ret signalling integrates a craniofacial muscle module during development

Ret signalling integrates a craniofacial muscle module during development

... (Degenhardt and Sassoon, 2001). The basic helix-loop-helix genes capsulin (Caps; Tcf21 – Mouse Genome Informatics) and musculin are expressed in all pharyngeal arch muscles, but double mutant mice lack masticatory ...

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The myogenic regulatory factors: critical determinants of muscle identity in development, growth and regeneration

The myogenic regulatory factors: critical determinants of muscle identity in development, growth and regeneration

... for myogenic differentiation ...repressed myogenic genes which have promoter elements inaccesible to transcription factor binding due to their chromatin ...preparing cells for subsequent inductive ...

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Unravelling the role of mechanical cues on the chondrogenic differentiation of stem cells

Unravelling the role of mechanical cues on the chondrogenic differentiation of stem cells

... primed cells for a robust response to the transforming growth factor (TGF)-β, which plays a major role in the regulation of ...when cells were grown on ...these cells were also supplemented with ...

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The effect of the muscle environment on the regenerative capacity of human skeletal muscle stem cells

The effect of the muscle environment on the regenerative capacity of human skeletal muscle stem cells

... stem cells need to be extensively tested in la- boratory animal models to elucidate their suitability for clinical application, and it is important that an appropri- ate animal model is ...stem cells of hu- ...

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Generation of myogenic progenitor cell-derived smooth muscle cells for sphincter regeneration

Generation of myogenic progenitor cell-derived smooth muscle cells for sphincter regeneration

... Ham’s F12 and covered with each 5 ml adipogenic, chondrogenic, or osteogenic differentiation medium. Adipogenic, chondrogenic, and osteogenic differentiation medium consisted of StemXVivo™ Osteogenic/Adipo- genic Base ...

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