NADPH and NADH
Nonequivalence of second sphere "noncatalytic" residues in pentaerythritol tetranitrate reductase in relation to local dynamics linked to H-transfer in reactions with NADH and NADPH coenzymes
... with NADH and is noticeably less e ffi cient for performing H-transfer from ...both NADPH and NADH coenzymes, along with their corresponding deuterated forms, in the range of 5 − 40 ° ...
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Comparison of NADH and NADPH oxidase activities in granules isolated from human polymorphonuclear leukocytes with a fluorometric assay
... Both NADPH and NADH oxidase activities were insensitive to ...cyanide. NADPH oxidation had a pH optimum of 5.5, while that for NADH appeared to be ...of NADPH was routinely four to five ...
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Redox engineering by ectopic expression of glutamate dehydrogenase genes links NADPH availability and NADH oxidation with cold growth in Saccharomyces cerevisiae
... reduced NADPH availability by knock‑out or overexpression of GRE3, the NADPH‑dependent aldose reductase gene, eliminated or exacerbated the cold‑growth defect observed in YEpGDH1 ...increasing NADH ...
11
Angiotensin II mediated hypertension in the rat increases vascular superoxide production via membrane NADH/NADPH oxidase activation Contribution to alterations of vasomotor tone
... favors NADPH, while that of the vascular cell favors NADH (this study and references 13, 19, 21, ...neutrophil/macrophage NADPH oxidase, may use some of the same components as the neutrophil oxi- ...
9
Enhanced lincomycin A production by calcium gluconate feeding in fermentation of Streptomyces lincolnensis
... metabolites, NADPH and NADH concentrations, and NADPH/NADH ratio were determined to investigate the mechanism of enhanced linco- mycin A production by calcium gluconate ...of ...
9
Engineering a cyanobacterium as the catalyst for the photosynthetic conversion of CO2 to 1,2-propanediol
... yqhD, NADPH-dependent methylglyoxal reduction activities were also measured (See Additional file 1: Figure S1 and text in supporting material), although assay method with higher specificity needs to be ...(C) ...
9
Anaerobic poly-3-d-hydroxybutyrate production from xylose in recombinant Saccharomyces cerevisiae using a NADH-dependent acetoacetyl-CoA reductase
... an NADPH-dependent AAR gene, whereas AAR with putative promiscuous cofactor utilization are only reported in few cases: for the halotolerant bacterium Allochromatium vinosum, formerly known as Chroma- tium vinosum ...
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Enhanced direct fermentation of cassava to butanol by Clostridium species strain BOH3 in cofactor-mediated medium
... of NADH and NADPH were lacking at the node of alcohol-depend- ent ...Interestingly, NADH and NADPH de novo synthesis in the presence of l-tryptophan complemented the availability and levels of ...
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Paraquat increases cyanide insensitive respiration in murine lung epithelial cells by activating an NAD(P)H:paraquat oxidoreductase: identification of the enzyme as thioredoxin reductase
... and NADH- or NADPH-dependent, readily catalyzes the generation of the characteristic blue-violet paraquat radical (see Reaction 1) and is distinct from cytochrome P450 ...utilizing NADH as a source ...
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Purification and Identification of an FMN-dependent NAD(P)H Azoreductase from Enterococcus faecalis
... only NADH as an electron ...involves NADPH as well as an electron ...other NADPH-specific azoreductases or altered activity caused by the heterologously expressed ...
8
Boosting the biosynthesis of betulinic acid and related triterpenoids in Yarrowia lipolytica via multimodular metabolic engineering
... the NADPH or NADH supply by overexpres- sion of malic enzyme or glyceraldehyde-3-phosphate (G3P) dehydrogenase, ...for NADH generation, was overexpressed, triter- penoid production decreased ...the ...
18
Carbonyl reductase identification and development of whole-cell biotransformation for highly efficient synthesis of (R)-[3,5-bis(trifluoromethyl)phenyl] ethanol
... With the increased availability of public genome information, many putative carbonyl reductases can be obtained from GenBank (http://www.ncbi.nlm.nih.gov/ genbank/). In this study, a NADPH-dependent carbonyl ...
11
Activation of biliverdin IXα reductase by inorganic phosphate and related anions
... the incubation mixture contained 0.1 M sodium phosphate buffer (pH 7.2), 700 µM NADH and 10 µM biliverdin-IXα, and the reaction was started by the addition of enzyme. To determine the stability of BVR-A over the ...
7
POS5 Gene of Saccharomyces cerevisiae Encodes a Mitochondrial NADH Kinase Required for Stability of Mitochondrial DNA
... The vast majority of oxygen consumed by eukaryotic cells is utilized for oxidative phosphorylation within the mitochondria. Although cells balance the stream of reducing equivalents and oxygen to avoid the injurious ...
12
Manganese oxide biomineralization is a social trait protecting
... NADH/NADPH for hydrogen peroxide reduction 18, 19, MnOX at its different oxidation states.. II, III, IV can, once formed, directly catalyze degradation of hydrogen peroxide without NADH.[r] ...
36
Hydralazine prevents nitroglycerin tolerance by inhibiting activation of a membrane bound NADH oxidase A new action for an old drug
... The mechanism whereby nitroglycerin treatment increases activity of NADH/NADPH-dependent oxidases remains un- clear. However, it may involve activation by neurohumoral stimuli, such as angiotensin II. ...
7
Efficient one-step production of (S)-1-phenyl-1,2-ethanediol from (R)-enantiomer plus NAD+–NADPH in-situ regeneration using engineered Escherichia coli
... , NADH, NADP + , and NADPH, and their ratios, in the recombinant cells in the exponential growth ..., NADPH, and NADP + ) had different concentrations in the different ...the NADH and NAD + ...
9
Methanol fermentation increases the production of NAD(P)H-dependent chemicals in synthetic methylotrophic Escherichia coli
... that NADH was a potent kinetic inhibitor of this ...of NADH could inhibit MDH activity by ~ 50% ...extra NADH to generate NADPH for lysine production, and largely decreased the intracel- lular ...
11
Cellular and molecular mechanisms of endothelial cell dysfunction
... use NADH and NADPH as substrates for electron transfer to molecular oxygen ...neutrophil NADPH oxidase (53, 54) in that they possess flavin- and heme-binding regions that are likely important in the ...
6
Model-driven intracellular redox status modulation for increasing isobutanol production in Escherichia coli
... (1.944), the reaction GAPDH was identified as the most important target. Similarly, the GAPDH was also pre- dicted by the reference [11] to be the best cofactor-swap target in E. coli for enhancing the theoretical yields ...
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